Innovations in CAZyme gene diversity and its modification for biorefinery applications

Chettri, Dixita; Verma, Ashwani Kumar; Verma, Anil Kumar

doi:10.1016/j.btre.2020.e00525

Cited by 41 publications

(30 citation statements)

References 230 publications

(223 reference statements)

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“…Although there are many papers on CAZymes in the biorefinery context (Chettri et al, 2020 ; Li et al, 2020 ; Meng et al, 2020 ), in this editorial, we focus on the works published in this research topic. We would like to thank all the authors that have contributed to this special feature series of articles on CAZymes in Biorefinery.…”

mentioning

confidence: 99%

Editorial: CAZymes in Biorefinery: From Genes to Application

Contesini

Frandsen

Damásio

2021

Front. Bioeng. Biotechnol.

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confidence: 99%

Editorial: CAZymes in Biorefinery: From Genes to Application

Contesini

Frandsen

Damásio

2021

Front. Bioeng. Biotechnol.

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“…Microbes and their carbohydrate-active enzymes (CAZymes) are central for depolymerization of the complex lignocellulosic polysaccharides in the global carbon cycle as well as in industrial bioconversion processes [ 13 ]–[ 15 ]. Complete or semi-complete enzymatic breakdown of biomass requires multiple exo-, endo- and auxiliary CAZymes to hydrolyze the diversity of polysaccharide backbones and side chains [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

CAZyme prediction in ascomycetous yeast genomes guides discovery of novel xylanolytic species with diverse capacities for hemicellulose hydrolysis

Ravn

Engqvist

Larsbrink

et al. 2021

Biotechnol Biofuels

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Background Ascomycetous yeasts from the kingdom fungi inhabit every biome in nature. While filamentous fungi have been studied extensively regarding their enzymatic degradation of the complex polymers comprising lignocellulose, yeasts have been largely overlooked. As yeasts are key organisms used in industry, understanding their enzymatic strategies for biomass conversion is an important factor in developing new and more efficient cell factories. The aim of this study was to identify polysaccharide-degrading yeasts by mining CAZymes in 332 yeast genomes from the phylum Ascomycota. Selected CAZyme-rich yeasts were then characterized in more detail through growth and enzymatic activity assays. Results The CAZyme analysis revealed a large spread in the number of CAZyme-encoding genes in the ascomycetous yeast genomes. We identified a total of 217 predicted CAZyme families, including several CAZymes likely involved in degradation of plant polysaccharides. Growth characterization of 40 CAZyme-rich yeasts revealed no cellulolytic yeasts, but several species from the Trichomonascaceae and CUG-Ser1 clades were able to grow on xylan, mixed-linkage β-glucan and xyloglucan. Blastobotrys mokoenaii, Sugiyamaella lignohabitans, Spencermartinsiella europaea and several Scheffersomyces species displayed superior growth on xylan and well as high enzymatic activities. These species possess genes for several putative xylanolytic enzymes, including ones from the well-studied xylanase-containing glycoside hydrolase families GH10 and GH30, which appear to be attached to the cell surface. B. mokoenaii was the only species containing a GH11 xylanase, which was shown to be secreted. Surprisingly, no known xylanases were predicted in the xylanolytic species Wickerhamomyces canadensis, suggesting that this yeast possesses novel xylanases. In addition, by examining non-sequenced yeasts closely related to the xylanolytic yeasts, we were able to identify novel species with high xylanolytic capacities. Conclusions Our approach of combining high-throughput bioinformatic CAZyme-prediction with growth and enzyme characterization proved to be a powerful pipeline for discovery of novel xylan-degrading yeasts and enzymes. The identified yeasts display diverse profiles in terms of growth, enzymatic activities and xylan substrate preferences, pointing towards different strategies for degradation and utilization of xylan. Together, the results provide novel insights into how yeast degrade xylan, which can be used to improve cell factory design and industrial bioconversion processes.

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“…Most of the exogenous enzymes used in animal feed are of fungal origin. Indeed, the use of fungi in the degradation of plant raw material is well recognized as they secrete a rich panel of enzymes (i.e., secretome) able to degrade complex biomass [ 17 , 18 , 19 , 20 , 21 , 22 ]. Fungi can adapt their secretomes depending on environmental conditions, including the growth substrate, temperature, and growth phases [ 20 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Secretomes of Aspergillus japonicus and Aspergillus terreus Supplement the Rovabio® Enzyme Cocktail for the Degradation of Soybean Meal for Animal Feed

Grandmontagne

Navarro

Neugnot‐Roux

et al. 2021

JoF

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One of the challenges of the 21st century will be to feed more than 10 billion people by 2050. In animal feed, one of the promising approaches is to use agriculture by-products such as soybean meal as it represents a rich source of proteins. However, soybean meal proteins are embedded in a complex plant cell wall matrix, mostly composed of pectic polysaccharides, which are recalcitrant to digestion for animals and can cause digestive disorders in poultry breeding. In this study, we explored fungal diversity to find enzymes acting on soybean meal components. An exploration of almost 50 fungal strains enabled the identification of two strains (Aspergillus terreus and Aspergillus japonicus), which improved the solubilization of soybean meal in terms of polysaccharides and proteins. The two Aspergilli strains identified in the frame of this study offer a promising solution to process industrial food coproducts into suitable animal feed solutions.

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Innovations in CAZyme gene diversity and its modification for biorefinery applications

Cited by 41 publications

References 230 publications

Editorial: CAZymes in Biorefinery: From Genes to Application

Editorial: CAZymes in Biorefinery: From Genes to Application

CAZyme prediction in ascomycetous yeast genomes guides discovery of novel xylanolytic species with diverse capacities for hemicellulose hydrolysis

The Secretomes of Aspergillus japonicus and Aspergillus terreus Supplement the Rovabio® Enzyme Cocktail for the Degradation of Soybean Meal for Animal Feed

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