Innovative low-cost biosorption process of Cr6+ by Pseudomonas alcaliphila NEWG-2

Abstract

Chromium is one of the heavy metal pollutants that causing risky health issues when discharged into the aquatic ecosystems. The current investigation focused on the bioremoval of Cr 6+ depending on the bacterial sorption process by using Pseudomonas sp. NEWG-2 which was identified on the basis of morphological, cultural characteristics, 16S rRNA sequencing and phylogenetic analysis as Pseudomonas alcaliphila strain NEWG-2. It is clear from the FCCD experiments that the bacterium can grow normally and remove 96.60% of 200 mg/l of Cr 6+ using yeast extract (5.6 g/l), glucose (4.9 g/l), pH (7) for 48 h incubation period. SEM and EDS analyses proved that the Cr 6+ was biosorbed by P. alcaliphila NEWG-2. FTIR spectra indicated that the phenolic, carbonyl ester, acetyl, carboxylate, alkanes and carbonyl were the main groups involved in the chromium biosorption. Of the equilibrium isotherms models, the Langmuir model was more obedient, with a maximum uptake ( q max ) of 10 mg/g (bacterial-alginate beads), than the Freundlich one. The findings reveal the efficiency of P. alcaliphila NEWG-2 in Cr 6+ biosorption, with feasibility in the treatment of chromium-contaminated water as a green-technology tool. Interestingly, to the best of our knowledge, this is the first report on Cr 6+ biosorption process by P. alcaliphila .

Rotatable central composite design versus artificial neural network for modeling biosorption of Cr6+ by the immobilized Pseudomonas alcaliphila NEWG-2

“…On the other side, the role of sodium alginate beads alone was stated and was found to absorb some of the heavy metals, e.g., Cr 6+ 16 and Cu 2+ 11 ions. The sodium alginate is a natural polymer produced by marine algae, with a chemical structure of mannuronate and guluronate arranged with 1, 4-linkage.…”

“…In a previous study, P. alcaliphila NEWG-2 was found to be efficient in biosorption of Cr 6+ and molecularly identified with an accession number of MN025267 16 . This strain was used during the current investigation.…”

“…Immobilized cells had been also pronounced in several fields of the environmental, pharmaceutical, and food industry, for instance, immobilized cells of Azotobacter nigricans were efficient in Cu 2+ removal 11 . Likewise, EL-Naggar et al 16 investigating the role of immobilized cells of P . alcaliphila in removing Cr 6+ during Langmuir and Freundlich modeling.…”

“…Modeling by response surface methodology (RSM) has been extensively applied to assess the simultaneous influencing response factors and their interaction within the tested range, using a limited number of trials, Box–Behnken and central composite designs are two major sorts of RSM 11 , 16 . RSM is a worthwhile statistical mathematical system utilized for improving the experimental conditions through the discovery of the analytical relationship between inputs and outputs 17 .…”

Eco-friendly approach for biosorption of Pb2+ and carcinogenic Congo red dye from binary solution onto sustainable Ulva lactuca biomass

“…Statistical optimization of simultaneous bioremoval of Pb 2+ and congo red dye from binary solution by Ulva lactuca biomass.. Biosorption processes are affected by many environmental factors such as initial pH level, biosorbent dosage, contact time, temperature and initial metal concentration 39 . Optimization of biosorption process parameters was carried out using the Face-centered central composite design (FCCCD) to maximize the removal percentages and to study the individual, interaction and quadratic effects of process variables on simultaneous bioremoval of Pb 2+ and Congo red dye from binary solution by dry Ulva lactuca biomass.…”