Inoculation technology for legumes based on alginate encapsulation

Щербакова, Е. Н.; Shcherbakov, Andrey; Rots, P.Yu.; Gonchar, L.N.; Mulina, S.A.; Yahina, L.M.; Lactionov, Yu.V.; Chebotar, Vladimir

doi:10.15159/ar.18.186

Cited by 4 publications

(4 citation statements)

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“…The cell numbers for the nitrogen-fixing B. japonicum (Figure 2B) were 1.1 × 10 8 by the end of the year of storage. This is in agreement with similar studies 21 and confirms that the developed formulation, containing nitrogen assimilating bacteria, allows for stable preservation during longterm storage and creates an opportunity for application in agriculture. 22,23 To date, other studies have reported that Azospirillum lipoferum and Bacillus subtilis, for example, remained viable after respective 1 and 3 years of storage, but only when stabilized in alginate matrix that limited mechanical stress and mortality in comparison to conventional liquid formulations.…”

Section: ■ Materials and Methodssupporting

confidence: 92%

Assessment of Long-Term Stability of Encapsulated Agricultural Biologicals in Lipid-Coated Alginate Beads

Kucharzyk

Fulwider

Duong

et al. 2023

ACS Agric. Sci. Technol.

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Recent shift in preference of biological products over chemical seed treatments has resulted in an increased requirement for development of formulations to improve viability and consistency of microbial inoculants. Two of the biggest challenges in introducing biological products to the agriculture industry are stability of formulations and compatibility with common agriculture chemicals. In this study, multiple alginate encapsulation formulations were examined to not only increase shelf stability of microbes but also to improve their protection from various environmental factors. The initial alginate formulation extended stability of sensitive organisms to one year for both on shelf and on seed. The addition of a hydrophobic coating to the formulation protected cells from agriculture chemicals permitting at least three months of survival on shelf and on seed, with little to no viability loss after chemical exposure, in comparison to free microbials. This improved formulation is scalable for field application and provides a new platform for increased viability of agriculturally relevant microorganisms.

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Section: ■ Materials and Methodssupporting

confidence: 92%

Assessment of Long-Term Stability of Encapsulated Agricultural Biologicals in Lipid-Coated Alginate Beads

Kucharzyk

Fulwider

Duong

et al. 2023

ACS Agric. Sci. Technol.

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“…In the field, the inoculation of capsules of different bacteria of the genus Bacillus, Azospirillum and Burkholderia increased the concentration of nutrients, such as nitrogen and phosphorus, in Eugenia stipitata plants [119]. Microcapsules containing Mesorhizobium ciceri ST-282 and Bradyrhizobium japonicum M8 increased the number of nodules in roots of chickpea and soybean plants in a field experiment [122]. The encapsulation of T. viride increased the content of secondary metabolites in lettuce plants, when grown in the field and also in hydroponic culture [125], highlighting the importance of encapsulating beneficial microorganisms, including both fungi bacteria in different cultivation techniques.…”

Section: Benefits Of Bioencapsulation In the Fieldmentioning

confidence: 97%

“…[139]. Using alginate with gelatin, pectin, kaolin or bentonite to encapsulate Mesorhizobium ciceri and Bradyrhizobium japonicum increased the number of nodules formed in plants of Cicer arietinum (chickpea) and Glycine max (soybean), in comparison with non-inoculated plants [122].…”

Section: Alginatementioning

confidence: 99%

Bioencapsulation of Microbial Inoculants: Mechanisms, Formulation Types and Application Techniques

Rojas-Sánchez

Guzmán-Guzmán

Morales-Cedeño

et al. 2022

Applied Biosciences

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The excessive use of agrochemicals in the field to increase production and counteract the negative effects caused by biotic and abiotic factors has led to a deterioration in soil fertility, plus an increment in negative impacts on the environment and human health. Therefore, the application of beneficial microorganisms as bioinoculants is an eco-friendly alternative to agrochemicals. Plant growth-promoting bacteria and fungi have been effective in promoting plant growth and production, as well as reducing the action of pathogens in multiple crops. However, successful application of such beneficial microorganisms in the agricultural field has faced several difficulties, such as survival, colonization efficiency and short periods of shelf storage. Therefore, it is essential to explore novel ways to encapsulate, formulate and apply bioinoculants. To obtain the expected quality in bioencapsulated products, it is essential to determine the type of polymer, capsule size, encapsulation technique and use the correct chemical and physical cofactors involved in the production process. Thus, this review highlights the various formulation types and application techniques, as well as discussing the multiple advantages of using microbial encapsulates to have better results in agricultural production.

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“…Even though the “initial burst” behavior is the most common one for the release of encapsulated bacteria, the opposite phenomenon can also be observed [ 65 , 66 ]. This less common release pattern is characterized by a first period of very slow release (after 10 days, the beads studied by Shcherbakova et al [ 65 ] only released 5–8% of their initial content). The burst occurs after this initial phase; after 20 days, the bacteria were mainly out of the beads.…”

Section: Diffusion Phenomena and Controlled Releasementioning

confidence: 99%

Encapsulation in Alginates Hydrogels and Controlled Release: An Overview

Colin,

Akpo,

Perrin

et al. 2024

Molecules

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This review aims to gather the current state of the art on the encapsulation methods using alginate as the main polymeric material in order to produce hydrogels ranging from the microscopic to macroscopic sizes. The use of alginates as an encapsulation material is of growing interest, as it is fully bio-based, bio-compatible and bio-degradable. The field of application of alginate encapsulation is also extremely broad, and there is no doubt it will become even broader in the near future considering the societal demand for sustainable materials in technological applications. In this review, alginate’s main properties and gelification mechanisms, as well as some factors influencing this mechanism, such as the nature of the reticulation cations, are first investigated. Then, the capacity of alginate gels to release matter in a controlled way, from small molecules to micrometric compounds, is reported and discussed. The existing techniques used to produce alginates beads, from the laboratory scale to the industrial one, are further described, with a consideration of the pros and cons with each techniques. Finally, two examples of applications of alginate materials are highlighted as representative case studies.

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Inoculation technology for legumes based on alginate encapsulation

Cited by 4 publications

References 0 publications

Assessment of Long-Term Stability of Encapsulated Agricultural Biologicals in Lipid-Coated Alginate Beads

Assessment of Long-Term Stability of Encapsulated Agricultural Biologicals in Lipid-Coated Alginate Beads

Bioencapsulation of Microbial Inoculants: Mechanisms, Formulation Types and Application Techniques

Encapsulation in Alginates Hydrogels and Controlled Release: An Overview

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