Inoculation with plant growth-promoting bacteria alters the rhizosphere functioning of tomato plants

Zuluaga, Mónica Yorlady Alzate; Milani, Karina Maria Lima; Miras-Moreno, Begoña; Lucini, Luigi; Valentinuzzi, Fabio; Mimmo, Tanja; Pii, Youry; Cesco, Stefano; Rodrigues, Elisete Pains; Oliveira, André Luiz Martinez de

doi:10.1016/j.apsoil.2020.103784

Cited by 45 publications

(19 citation statements)

References 72 publications

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“…In this study, regardless of P supply, the inoculation with the bacterial biostimulant significantly reduced flavonoid content in the root exudates of cucumber plants, while their concentration in maize exudates was not affected, except for the treatment 15SIP (P–); in this case, a reduction of flavonoids was induced as also observed for phenolic compounds. A differential accumulation of flavonoids was described by Zuluaga et al ( 2021 ) in root exudates of tomato plants inoculated with the same Enterobacter 15S strain used in the present study. In some cases, the down-accumulation of flavonoids in PGPB-inoculated plants may indicate its consumption as a C source or a blocking in their biosynthesis (Dardanelli et al, 2010 ; Wu et al, 2018 ).…”

Section: Discussionsupporting

confidence: 78%

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

et al. 2021

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Phosphorus (P) is an essential nutrient for plants. The use of plant growth-promoting bacteria (PGPB) may also improve plant development and enhance nutrient availability, thus providing a promising alternative or supplement to chemical fertilizers. This study aimed to evaluate the effectiveness of Enterobacter sp. strain 15S in improving the growth and P acquisition of maize (monocot) and cucumber (dicot) plants under P-deficient hydroponic conditions, either by itself or by solubilizing an external source of inorganic phosphate (Pi) [Ca3(PO4)2]. The inoculation with Enterobacter 15S elicited different effects on the root architecture and biomass of cucumber and maize depending on the P supply. Under sufficient P, the bacterium induced a positive effect on the whole root system architecture of both plants. However, under P deficiency, the bacterium in combination with Ca3(PO4)2 induced a more remarkable effect on cucumber, while the bacterium alone was better in improving the root system of maize compared to non-inoculated plants. In P-deficient plants, bacterial inoculation also led to a chlorophyll content [soil-plant analysis development (SPAD) index] like that in P-sufficient plants (p < 0.05). Regarding P nutrition, the ionomic analysis indicated that inoculation with Enterobacter 15S increased the allocation of P in roots (+31%) and shoots (+53%) of cucumber plants grown in a P-free nutrient solution (NS) supplemented with the external insoluble phosphate, whereas maize plants inoculated with the bacterium alone showed a higher content of P only in roots (36%) but not in shoots. Furthermore, in P-deficient cucumber plants, all Pi transporter genes (CsPT1.3, CsPT1.4, CsPT1.9, and Cucsa383630.1) were upregulated by the bacterium inoculation, whereas, in P-deficient maize plants, the expression of ZmPT1 and ZmPT5 was downregulated by the bacterial inoculation. Taken together, these results suggest that, in its interaction with P-deficient cucumber plants, Enterobacter strain 15S might have solubilized the Ca3(PO4)2 to help the plants overcome P deficiency, while the association of maize plants with the bacterium might have triggered a different mechanism affecting plant metabolism. Thus, the mechanisms by which Enterobacter 15S improves plant growth and P nutrition are dependent on crop and nutrient status.

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Section: Discussionsupporting

confidence: 78%

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

et al. 2021

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“…The current study reports on the rhizosphere exo-metabolome profile inclusive of phenylpropanoids and relative polyketides. Based on the above information, it can be speculated that plants exude these secondary metabolites into the rhizosphere to serve as chemotaxis strategies for the recruitment of beneficial rhizosphere-inhabiting microbes to shape the rhizosphere microbial community and facilitate plant–microbe interactions ( Tilston et al, 2014 ; Zuluaga et al, 2021 ). In support of this speculation, it was observed that the rhizosphere of untreated Gariep cultivars contained elevated concentrations of phenylpropanoids compared to their PGPR- ( B. sub and P. alvei )-treated counterparts.…”

Section: Discussionmentioning

confidence: 99%

Untargeted metabolite profiling to elucidate rhizosphere and leaf metabolome changes of wheat cultivars (Triticum aestivum L.) treated with the plant growth-promoting rhizobacteria Paenibacillus alvei (T22) and Bacillus subtilis

et al. 2022

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The rhizosphere is a highly complex and biochemically diverse environment that facilitates plant–microbe and microbe–microbe interactions, and this region is found between plant roots and the bulk soil. Several studies have reported plant root exudation and metabolite secretion by rhizosphere-inhabiting microbes, suggesting that these metabolites play a vital role in plant–microbe interactions. However, the biochemical constellation of the rhizosphere soil is yet to be fully elucidated and thus remains extremely elusive. In this regard, the effects of plant growth-promoting rhizobacteria (PGPR)–plant interactions on the rhizosphere chemistry and above ground tissues are not fully understood. The current study applies an untargeted metabolomics approach to profile the rhizosphere exo-metabolome of wheat cultivars generated from seed inoculated (bio-primed) with Paenibacillus (T22) and Bacillus subtilis strains and to elucidate the effects of PGPR treatment on the metabolism of above-ground tissues. Chemometrics and molecular networking tools were used to process, mine and interpret the acquired mass spectrometry (MS) data. Global metabolome profiling of the rhizosphere soil of PGPR-bio-primed plants revealed differential accumulation of compounds from several classes of metabolites including phenylpropanoids, organic acids, lipids, organoheterocyclic compounds, and benzenoids. Of these, some have been reported to function in plant–microbe interactions, chemotaxis, biocontrol, and plant growth promotion. Metabolic perturbations associated with the primary and secondary metabolism were observed from the profiled leaf tissue of PGPR-bio-primed plants, suggesting a distal metabolic reprograming induced by PGPR seed bio-priming. These observations gave insights into the hypothetical framework which suggests that PGPR seed bio-priming can induce metabolic changes in plants leading to induced systemic response for adaptation to biotic and abiotic stress. Thus, this study contributes knowledge to ongoing efforts to decipher the rhizosphere metabolome and mechanistic nature of biochemical plant–microbe interactions, which could lead to metabolome engineering strategies for improved plant growth, priming for defense and sustainable agriculture.

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“…One way to increase microbiome-associated multifunctionality is the introduction of additional microbes into the soil [6]. For a long time, microorganisms have been selected on the base of their ability to directly express functions of interests [7], such as nutrient mineralisation, nitrogen fixation or pathogen suppression [8].…”

Section: Introductionmentioning

confidence: 99%

Introduction of probiotic bacterial consortia promotes plant growth via impacts on the resident rhizosphere microbiome

Yang

Friman

et al. 2021

Preprint

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Plant growth depends on a range of functions provided by their associated rhizosphere microbiome, including nutrient mineralization, hormone co-regulation and pathogen suppression. Improving the ability of plant associated microbiome to deliver these functions is thus important for developing robust and sustainable crop production. However, it is yet unclear how beneficial effects of probiotic microbial inoculants can be optimised and how they are mediated. Here, we sought to enhance the tomato plant growth by targeted introduction of probiotic bacterial consortia consisting of up to eight plant-associated Pseudomonas strains. We found that the effect of probiotic consortium inoculation was richness-dependent: consortia that contained more Pseudomonas strains, reached higher densities in the tomato rhizosphere and had clearer beneficial effects on multiple plant growth characteristics. Crucially, these effects were best explained by changes in the resident community diversity, composition and increase in the relative abundance of initially rare taxa, instead of introduction of plant-beneficial traits into the existing community along with probiotic consortia. Together, our results suggest that beneficial effects of microbial introductions can be driven indirectly through effects on the diversity and composition of resident plant rhizosphere microbiome.

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Inoculation with plant growth-promoting bacteria alters the rhizosphere functioning of tomato plants

Cited by 45 publications

References 72 publications

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

Untargeted metabolite profiling to elucidate rhizosphere and leaf metabolome changes of wheat cultivars (Triticum aestivum L.) treated with the plant growth-promoting rhizobacteria Paenibacillus alvei (T22) and Bacillus subtilis

Introduction of probiotic bacterial consortia promotes plant growth via impacts on the resident rhizosphere microbiome

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