Inorganic and organic fertilizers impact the abundance and proportion of antibiotic resistance and integron-integrase genes in agricultural grassland soil

Nõlvak, Hiie; Truu, Marika; Kanger, Kärt; Tampere, M.; Espenberg, Mikk; Loit, Evelin; Raave, H.; Truu, Jaak

doi:10.1016/j.scitotenv.2016.04.035

Cited by 111 publications

(50 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ARB and ARGs can persist and multiply, and ARGs can spread by horizontal gene transfer (HGT) mediated by mobile genetic elements (MGE) within and beyond the original point of occurrence [2], making possible the exchange of resistance traits between environmental bacteria and human pathogens [3]. ARGs and MGE can be enriched in natural ecosystems impacted by human activities such as wastewater discharge [4], solid waste disposal [5,6], metal contamination [7,8], agriculture [9,10,11], aquaculture [12], and drug manufacture [13]. However, very little is known about environmental contamination with ARB and ARGs in developing countries, especially in sub-Saharan Africa.…”

Section: Introductionmentioning

confidence: 99%

High abundances of class 1 integrase and sulfonamide resistance genes, and characterisation of class 1 integron gene cassettes in four urban wetlands in Nigeria

et al. 2018

View full text Add to dashboard Cite

There is little information about environmental contamination with antibiotic resistance genes (ARG) in Sub-Saharan Africa, home to about 1 billion people. In this study we measured the abundance of three genes (sul1, sul2, and intI1) used as indicators of environmental contamination with ARGs in the sediments of four urban wetlands in southwestern Nigeria by qPCR. In addition, we characterised the variable regions of class 1 integrons in sulfamethoxazole/trimethoprim (SMX/TRI)-resistant bacteria isolated from the wetlands by PCR and DNA sequencing. The indicator ARGs were present in all wetlands with mean absolute copy numbers/gram of sediment ranging between 4.7x10 6 and 1.2x10 8 for sul1, 1.1x10 7 and 1x10 8 for sul2, and 5.3x10 5 and 1.9x10 7 for intI1. The relative abundances (ARG/16S rRNA copy number) ranged from about 10 −3 to 10 −1. These levels of ARG contamination were similar to those previously reported for polluted environments in other parts of the world. The integrase genes intI1 and intI2 were detected in 72% and 11.4% SMX/TRIresistant isolates, respectively. Five different cassette array types (dfrA7; aadA2; aadA1| dfrA1; acc(6')lb-cr|arr3|dfrA27; arr3|acc(6')lb-cr|dfrA27) were detected among 34 (59.6%) intI1-positive isolates. No gene cassettes were found in the nine intI2-positive isolates. These results show that African urban ecosystems impacted by anthropogenic activities are reservoirs of bacteria harbouring transferable ARG.

show abstract

Section: Introductionmentioning

confidence: 99%

High abundances of class 1 integrase and sulfonamide resistance genes, and characterisation of class 1 integron gene cassettes in four urban wetlands in Nigeria

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Nõlvak et al (2016) investigated the effect of inorganic and organic fertilizer on the abundance and proportion dynamics of ARGs and integron-integrase genes (i.e., intl (1) and intl (2)) in agricultural grassland soils. Targeted genes encoding resistance to sulfonamides ( sul (1)), tetracycline ( tet (A)), cephalosporins (blaCTX-M), penicillin (blaOXA2) and flouroquinolones ( qnr (S)) were analyzed as affected by mineral fertilizer application in comparison to cattle slurry and cattle slurry digestate.…”

Section: Antibiotic Resistance Genesmentioning

confidence: 99%

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

Snow¹,

Cassada²,

Larsen³

et al. 2017

Water Environment Research

View full text Add to dashboard Cite

A total of 79 papers published in 2016 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes, cyanotoxins, and potential effects of biochar use in agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Steroid Hormones, Anthelmintics, Antibiotic Fate and Occurrence, Antibiotic Resistance Genes, Cyanotoxins, and Implications of Biochar in Agricultural Environments.

show abstract

“…There is no systematic surveillance of antibiotic misuse and no data are available to quantify the problem (2,3). Currently, antibiotic resistance presents an increasing trend that leads to significant public health issues and the increase in healthcare-associated infections caused by antibiotic-resistant pathogens worldwide (4,5). Resistance to different antimicrobial drugs is attributed to the increased irregular employment of antibiotics that have resulted in the drug resistance of expression of virulence factors, promotion of intra-hospital cross infection and even increased challenges of clinical treatments (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Klebsiella pneumoniae presents antimicrobial drug resistance for β‑lactam through the ESBL/PBP signaling pathway

et al. 2020

View full text Add to dashboard Cite

Overuse and misuse of antibiotics leads to antibiotic resistance which has become a significant public health concern. Klebsiella pneumoniae is the most common pathogenic bacteria underlying nosocomial infections due to the expression of virulence factors and occurrence of antibiotic resistance. Evidence indicates that β-lactamase is involved in the antibiotic resistance of Klebsiella pneumoniae to β-lactam antibiotics. The aim of the present study was to investigate the association between the molecular biological mechanisms of antibiotic resistance of Klebsiella pneumoniae and extended-spectrum β-lactamase (ESBL). In order to assess temporal trends in prevalence and antimicrobial susceptibility, Klebsiella pneumoniae bacteria were isolated and the ESBL expression level was analyzed. Susceptibility tests were performed using automated systems. The β-lactam-resistance in Klebsiella pneumoniae was assessed by the β-lactam agar screen plate and respective MIC values were evaluated using E-test strips. The confirmatory disk diffusion methods were applied for phenotypic identification of the ESBL production of Klebsiella pneumoniae. The results showed that Klebsiella pneumoniae bacteria exhibited higher ESBL production after treatment with β-lactam compared to the control. The ESBL gene expression was upregulated in Klebsiella pneumoniae after treatment with β-lactam. Results identified that penicillin-binding proteins (PBPs) were associated with the growth and resistance to β-lactams. Zinc finger nuclease markedly inhibited the antibiotic resistance of Klebsiella pneumoniae to β-lactam. PBP knockdown abolished the inhibitory effects of zinc finger nuclease on the growth of Klebsiella pneumoniae induced by β-lactam antibiotic treatment. In conclusion, these results suggest that the resistance of Klebsiella pneumoniae bacteria to antimicrobial drugs is through the ESBL signaling pathway, which indicates that ESBL may be a potential target for abolishing resistance to β-lactam.

show abstract

Inorganic and organic fertilizers impact the abundance and proportion of antibiotic resistance and integron-integrase genes in agricultural grassland soil

Cited by 111 publications

References 42 publications

High abundances of class 1 integrase and sulfonamide resistance genes, and characterisation of class 1 integron gene cassettes in four urban wetlands in Nigeria

High abundances of class 1 integrase and sulfonamide resistance genes, and characterisation of class 1 integron gene cassettes in four urban wetlands in Nigeria

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

Klebsiella pneumoniae presents antimicrobial drug resistance for β‑lactam through the ESBL/PBP signaling pathway

Contact Info

Product

Resources

About