Insect cuticular proteins and their role in transmission of phytoviruses

Abstract

Graphical abstract

Molecular Insights into Host and Vector Manipulation by Plant Viruses

“…Whatever the virus retention site or the journey through the insect, transmission relies on specific interactions between viral proteins and vector proteins [5]. Viral determinants are particularly well characterized [2,4,6] while their vector counterparts, in particular the receptor cuticular proteins, are only beginning to be characterized [7].…”

Non-circulative virus transmission by Aphids : new insights into the mechanisms of transmission and the interference for retention sites in the vector

“…Even though peptide arrays within RR-1 domain were suggested for CMV interaction in M. persicae (Liang and Gao, 2017;Webster et al, 2018), peptide arrays of RR-2 protein family showed interaction with CMV binding in A. pisum (Webster et al, 2017). CMV actually interacts with both RR-1 proteins in yeast, and with RR-2 peptides in vitro, with both playing a role in virus retention (Deshoux et al, 2018). The interference observed in our experiments suggests that both ZYMV and CMV are using same receptors in A. gossypii stylets.…”

“…The attachment of NC virus particles to the specific retention sites in the aphid cuticle is exclusively mediated by protein interactions. The mechanisms undergoing NC virus-vector interactions as well as retention sites have been widely studied (Dombrovsky et al, 2005(Dombrovsky et al, , 2007Uzest et al, 2007;Uzest and Blanc, 2016;Webster et al, , 2018Deshoux et al, 2018) There are two molecular mechanisms involved in the retention of NC virus particles to specific attachment sites within their aphid vectors. Virus transmissibility and specificity depends on the coat protein (CP strategy) or other unique proteins that act as a bridge between the virion and aphid cuticle (helper strategy) (Raccah and Fereres, 2015;Uzest and Blanc, 2016).…”

“…Molecular studies have so far clarified the interaction of one protein of the RR-1 domain proteins directly involved in CaMV binding and also presumably to CMV (Webster et al, 2018;Deshoux et al, 2018) (Sylvester, 1956;Nault, 1997 (Godfrey and Mauk, 1993). BYV has an important effect on the physiology of infected plants by inducing leaf chlorosis, decreasing photosynthesis efficiency and thus reducing root yield (Clover et al, 1999).…”

“…Attempts to identify virus retention sites and putative virus receptors in the aphid stylets have been paramount recently in the field of plant-virus transmission (Liang and Gao, 2017;Webster et al, 2017;Webster et al, 2018;Deshoux et al, 2018;Fereres and Perry, 2019). However, few works were performed and little information was available at the beginning of the present PhD Thesis (2015).…”