Insecticidal Activity of Strains of Bacillus thuringiensis on Larvae and Adults of Bactrocera oleae Gmelin (Dipt. Tephritidae)

Alberola, Trinidad M.; Aptosoglou, Sofia; Arsenakis, Minas; Bel, Yolanda; Delrio, G.; Ellar, David J.; Ferré, Juan; Granero, Francisco; Guttmann, D; Koliais, Stefanos; Martínez-Sebastián, M. J.; Prota, R.; Rubino, Salvatore; Satta, Alberto; Scarpellini, Giorgio; Sivropoulou, Afroditi; Vasara, Eleni

doi:10.1006/jipa.1999.4871

Cited by 48 publications

(42 citation statements)

References 23 publications

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“…The 15.1 strain seems to be specific for the larval stage, since no toxicity for C. capitata adults was observed when the same conditions that resulted in maximum toxicity for larvae were used (unpublished results). Activity against only one of the possible insect stages has been observed previously for B. thuringiensis isolates that exhibit activity against larvae of other fruit flies (3,59). The 16S rRNA of the 15.1 strain was 100% identical to the 16S rRNA of several B. pumilus strains whose sequences have been deposited in the GenBank database.…”

Section: Discussionsupporting

confidence: 58%

Selection of a Bacillus pumilus Strain Highly Active against Ceratitis capitata (Wiedemann) Larvae

Molina

Caña-Roca

Osuna

et al. 2010

Appl Environ Microbiol

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Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), the Mediterranean fruit fly (medfly), is one of the most important fruit pests worldwide. The medfly is a polyphagous species that causes losses in many crops, which leads to huge economic losses. Entomopathogenic bacteria belonging to the genus Bacillus have been proven to be safe, environmentally friendly, and cost-effective tools to control pest populations. As no control method for C. capitata based on these bacteria has been developed, isolation of novel strains is needed. Here, we report the isolation of 115 bacterial strains and the results of toxicity screening with adults and larvae of C. capitata. As a result of this analysis, we obtained a novel Bacillus pumilus strain, strain 15.1, that is highly toxic to C. capitata larvae. The toxicity of this strain for C. capitata was related to the sporulation process and was observed only when cultures were incubated at low temperatures before they were used in a bioassay. The mortality rate for C. capitata larvae ranged from 68 to 94% depending on the conditions under which the culture was kept before the bioassay. Toxicity was proven to be a special characteristic of the newly isolated strain, since other B. pumilus strains did not have a toxic effect on C. capitata larvae. The results of the present study suggest that B. pumilus 15.1 could be considered a strong candidate for developing strategies for biological control of C. capitata.

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Section: Discussionsupporting

confidence: 58%

Selection of a Bacillus pumilus Strain Highly Active against Ceratitis capitata (Wiedemann) Larvae

Molina

Caña-Roca

Osuna

et al. 2010

Appl Environ Microbiol

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“…isolate 114A against B. oleae adults (Alberola et al 1999), several biotoxicity assays performed subsequently in our laboratory established its insecticidal activity against both adult and larvae of B. oleae. The mortality imposed by the endotoxins of this isolate on the olive pest was further investigated in laboratory experiments against the wild population of B. oleae as well as in Þeld experiments.…”

Section: Discussionmentioning

confidence: 99%

“…The biotoxicity of B. thuringiensis against B. oleae has been reported with laboratory reared larvae (Karamanlidou et al 1991) and more recently with the larvae and adult of B. oleae (Alberola et al 1999). We have also studied the abundance of B. thuringiensis in Greece (Aptosoglou et al 1997a) and the plasmid patterns among the B. thuringiensis isolates (Aptosoglou et al 1997b).…”

mentioning

confidence: 91%

Biological Control of <I>Bactocera oleae</I> (Diptera: Tephritidae) Using a Greek <I>Bacillus thuringiensis</I> Isolate

Navrozidis

Vasara

Karamanlidou

et al. 2000

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Bacillus thuringiensis, isolate 114A, was used in toxicity experiments against the wild population ofthe olive pest Bactroceria oleae (Gmelin). In laboratory experiments, spores and crystals of the B.t. were delivered to the insects with the food. Longevity, oviposition period, number of eggs produced, and percent hatch were recorded. Olive fruits from the oviposition test were dipped into a suspension containing spores and crystals of B. thuringiensis 114A after the eggs were deposited. In field experiments, four to six sprayings per year of B.t. 114A isolate were applied for three successive years. It was found that, in addition to the longevity of B. oleae, the oviposition period, number of eggs and percent egg hatch decreased. Also, the percentage of pupation and emergence was reduced when olive fruits with eggs in their mesocarp were dipped in the solution of spores and crystals. Field applications with the toxins of 114A isolate of B. thuringiensis have resulted in significant protection of the olive production.

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“…So far, the only effective method applied against A. orana is the use of synthetic insecticides such as azinphos-methyl + demeton-S-methyl sulphon, chlorpyriphos, permethrin and methidathion, but most of these are expensive with a high probability of residues. Use of the bioagent Bacillus thuringiensis against many pests has been reported (2,8,13,17), but its effectiveness is doubtful. Mating disruption is considered as a cornerstone of Integrated Pest Management in orchards (9).…”

Section: Introductionmentioning

confidence: 98%

Pheromone-based communication disruption ofAdoxophyes orana on peach using the new RAK 3+4 dispensers and their effect on development of fruit rot diseases

Navrozidis

Thomidis²,

Sipouridis³

et al. 2005

Phytoparasitica

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The effectiveness of the pheromone-based communication disruption method was examined against the summerfruit tortrix, Adoxophyes orana F.v.R. (Lepidoptera: Tortricidae), a pest of peach trees, using the new RAK 3+4 dispenser (BASF). No A. orana males were captured in pheromone traps inside the experimental orchards, which were saturated with the RAK 3+4 dispensers. The percent of damaged leaves was practically zero, while the level of damaged fruits was 0-6% in pheromone-treated orchards. The percentage of fruit rot caused by Monilinia laxa was lower in pheromone-based communication disruption orchards than in the control. It was concluded that the RAK 3+4 dispenser could be used against A. orana as an economical and environmentally friendly method.

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Insecticidal Activity of Strains of Bacillus thuringiensis on Larvae and Adults of Bactrocera oleae Gmelin (Dipt. Tephritidae)

Cited by 48 publications

References 23 publications

Selection of a Bacillus pumilus Strain Highly Active against Ceratitis capitata (Wiedemann) Larvae

Selection of a Bacillus pumilus Strain Highly Active against Ceratitis capitata (Wiedemann) Larvae

Biological Control of <I>Bactocera oleae</I> (Diptera: Tephritidae) Using a Greek <I>Bacillus thuringiensis</I> Isolate

Pheromone-based communication disruption ofAdoxophyes orana on peach using the new RAK 3+4 dispensers and their effect on development of fruit rot diseases

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