1975
DOI: 10.1111/j.1439-0531.1975.tb00275.x
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Insemination with frozen dog semen based on a new insemination technique

Abstract: Inhalt: (Insemination mit Gefriersperma beim Hund mit Hilfe einer neuen Besamungstechnik). In diesem Versuch sind 11 Hündinnen mit tiefgefrorenem Sperma besamt worden. Die spermienreiche Ejakulatsfraktion wurde unmittelbar nach der Samenentnahme mit einer Tris‐Fructose‐Zitronensüure‐Lösung, die 8 Vol. % Glyzerin und 20 Vol. % Eidotter enthielt, etwa 1: 4 verdünnt. Wührend einer 3stündigen Equilibrierungszeit wurde der Samen auf + 5 °C heruntergekühlt, and in PVC‐Röhrchen im Stickstoffdampf eingefroren. Di… Show more

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Cited by 77 publications
(52 citation statements)
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“…An equal volume of a second extender, consisting of the first extender supplemented with 16 v/v% glycerol, was then added at 4°C and samples were kept at the same temperature for the indicated times (equilibration). Thus, the final concentration of glycerol was always 8 v/v% [1,3].…”
Section: Methodsmentioning
confidence: 99%
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“…An equal volume of a second extender, consisting of the first extender supplemented with 16 v/v% glycerol, was then added at 4°C and samples were kept at the same temperature for the indicated times (equilibration). Thus, the final concentration of glycerol was always 8 v/v% [1,3].…”
Section: Methodsmentioning
confidence: 99%
“…On the other hand, if semen were collected from wild animals in the field, manipulation of the ejaculate would have to be carried out under unfavorable conditions. Thus, modifications would be needed for the application of techniques developed for dogs to wild animals.Successful artificial insemination with frozen canine semen has been well documented [18,22,23,25,27] since the first conception was reported by Seager [21], and recently, the freezing protocol for canine semen has been improved [1,3,20,[28][29][30]. If semen is diluted to a constant final sperm concentration, the dilution rates may differ.…”
mentioning
confidence: 99%
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“…Since the work of Anderson in 1975 [1], it was accepted that frozen-thawed dog sperm should be inseminated into the uterus in order to obtain good fertility. Uterine insemination poses practical problems because it either requires one to pass a pipette through the cervix, which is sometimes impossible to achieve [2] and [3], or expensive fiber-optic equipment with which to catheterize the cervix under visual control [4], or surgical or laparoscopic exposure of the uterus [5] and [6].…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, special equipment is needed for transcervical intrauterine AI. Norwegian catheters have been developed in the 1970s (Andersen, 1972(Andersen, , 1975 and are available in three different sizes to accommodate different sized bitches. For this technique, the cervix must be fixed through abdominal palpation and the catheter is moved slowly to find the cervical canal while the cervix is pulled downward.…”
Section: Wwwintechopencommentioning
confidence: 99%