Insensitivity of Acetylcholinesterase in Phenthoate Resistant Diamond-back Moth, Plutella xylostella L.(Lepidoptera : Yponomeutidae)

Noppun, Virapong; Miyata, Tadashi; Saitô, Tetsuo

doi:10.1303/aez.22.116

Cited by 10 publications

(4 citation statements)

References 5 publications

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“…In Southeastern Asian countries, the resistance problem has become more serious because DBM goes through its life cycle more than 15 times per year in this area. Several reports on OP resistance in DBM were reported (Cheng, 1986;Miyata et al, 1986;Sun et al, 1986) in the international workshop held in Tainan, Taiwan in 1985. As for the resistant mechanism, enhanced activities of carboxylesterase (Sun et al, 1978;Noppun et al, 1987) and glutathion S-transferase (Cheng et al, 1983) have been pointed out as degradation factors. A decrease in sensitivity of the target enzyme, acetylcholinesterase (AChE), to OP has also been proposed (Sudderuddin and Kok, 1978;Nop-pun et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

cDNA and deduced protein sequence of acetylcholinesterase from the diamondback moth, Plutella xylostella (L.)(Lepidoptera: Plutellidae).

Tomita

Kasai

et al. 2003

Appl. Entomol. Zool.

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The sequence of acetylcholinesterase (AChE) cDNA from the diamondback moth (DBM), Plutella xylostella (L.) was determined. A 2.3 kb partial cDNA sequence, containing an ORF encoding AChE precursor with 638 amino acid residues was obtained by primer walking. The deduced protein sequence showed the highest homology to AChE of the Colorado potato beetle (CPB) and the green rice leafhopper. The DBM AChE belongs to the Drosophila melanogaster Ace-orthologous insect AChE subfamily. A single AChE gene transcript (7.6 kb) was detected by Northern blot analysis. This is the first report of the complete coding sequence of AChE cDNA from a lepidopteran insect.

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Section: Introductionmentioning

confidence: 99%

cDNA and deduced protein sequence of acetylcholinesterase from the diamondback moth, Plutella xylostella (L.)(Lepidoptera: Plutellidae).

Tomita

Kasai

et al. 2003

Appl. Entomol. Zool.

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“…Inheritance of resistance to phenthoate in P. xylostella was derived by more than one gene, incomplete dominant genes and no sex linkage. (Noppun et al, 1987).…”

Section: Resultsmentioning

confidence: 99%

Dominant Inheritance of Plutella xylostella selected with Flufenoxuron

Diab

2015

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Dominance study was a petition needs to measure insecticide resistance problems to a notorious pest as Plutella xylostella and for IRM strategy planning success. Flufenoxuron insecticide was chosen for doing a laboratory selected strain for some resistance assessments. Studies on cross resistance of flufenoxuron resistant strains revealed a slight tolerance to lambdacyhalothrin, alphacypermethrin, thiocyclam and chlorfenapyr but no cross resistance to dimethoate, imidacloprid. Resistance stablility examined in both the flufenoxuron selected strain and the backcross selected x susceptible in the absence of exposure to insecticides, results revealed that the mortality were declined slowly at F3, F5 and F7. Results of reciprocal crossing experiments suggested that flufenoxuron resistance was inherited as an incomplete recessive trait. Values of the degree of dominance were estimated-0.63 and-0.66 for F1 larvae with resistant and susceptible female parents, respectively. Results of bioassays on F1 progeny of the backcrossed with the resistant parent strain and the F2 generations suggested that resistance autosomaly inherited and might be controlled by some loci. Analysis of probit lines from F1 reciprocal crosses indicated that resistance to flufenoxuron was inherited autosomaly as an incompletely recessive and probit lines showed no plateau at 50% mortality (probit = 4.0) and 3.4 for F2 at 25-75% mortality. The x 2 analysis of response ratio statistics of a monogenic model from F1 susceptible back crosses suggested that more than one locus is responsible for resistance to flufenoxuron.

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“…based products (Wright et al, 1997), as well as insect growth regulators (YuXian, 2001). Resistance mechanisms in DBM proposed for synthetic chemicals include decreased penetration (Noppun et al, 1987), enhanced detoxification by esterases (Maa and Chuang, 1983), glutathione-S-transferases and reduced sensitivity of acetylcholinesterase (Hama, 1987). Eziah et al (2008) have suggested based on a study on susceptibility of field populations of DBM to six selected insecticides, the involvement of esterases and/or monooxygenases as the key resistance mechanism.…”

Section: Introductionmentioning

confidence: 99%

Resistance of diamondback moth to insecticides in selected cabbage farms in southern Ghana

Odhiambo¹,

Gbewonyo

Ofori³

et al. 2011

International Journal of Biological and Chemical Sciences

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Resistance of diamondback moth (DBM), Plutella xylostella (L.) to insecticides applied for its control on cabbage was evaluated. DBM populations were tested for susceptibility to three pyrethroids (delatamethrin, lambdacyhalothrin, cypermethrin) and an organophosphate (chlorpyrifos-methyl) insecticide using larvae immersion dose-response technique. Non-specific carboxylesterase activity was used as a measure of DBM resistance. Partial characterization of carboxylesterase by zymogram studies was also undertaken using polyacrylamide gel electrophoresis (PAGE). Susceptibility studies revealed that DBM was resistant to the four insecticides assayed. DBM was least susceptible to chlorpyrifos-methyl, but most susceptible to deltamethrin. Esterase zymogram studies revealed between 2 to 7 bands, each with different characteristics. Higher frequency of the slow-moving esterases associated with high resistance was recorded in Mampong DBM population than the Accra suburbs. This finding confirmed results for carboxylesterase activity. Deltamethrin and cypermethrin demonstrated high negative correlation between fold resistance and carboxylesterase activity for β-isozyme. Chlorpyrifos-methyl, on the other hand showed positive correlation between fold resistance and α-carboxylesterase activity. The high carboxylesterase activities recorded for Mampong DBM, however, did not correlate with results of susceptibility studies. It is suggested that farmers should adopt an integrated approach using the IPM principles coupled with judicious use of biopesticides such as Bacillus thuringiensis.

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Insensitivity of Acetylcholinesterase in Phenthoate Resistant Diamond-back Moth, Plutella xylostella L.(Lepidoptera : Yponomeutidae)

Cited by 10 publications

References 5 publications

cDNA and deduced protein sequence of acetylcholinesterase from the diamondback moth, Plutella xylostella (L.)(Lepidoptera: Plutellidae).

cDNA and deduced protein sequence of acetylcholinesterase from the diamondback moth, Plutella xylostella (L.)(Lepidoptera: Plutellidae).

Dominant Inheritance of Plutella xylostella selected with Flufenoxuron

Resistance of diamondback moth to insecticides in selected cabbage farms in southern Ghana

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