Inside Cover: Internalization of Silica Nanoparticles into Fluid Liposomes: Formation of Interesting Hybrid Colloids (Angew. Chem. Int. Ed. 46/2014)

Michel, Raphaël; Kesselman, Ellina; Plostica, Tobias; Danino, Dganit; Gradzielski, Michael

doi:10.1002/anie.201410024

Cited by 16 publications

(23 citation statements)

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“…23,24 The processes of endocytosis and exocytosis can be mimicked in model systems, such as lipid or polymer vesicles in contact with adhesive particles, and the formation and closure of a narrow membrane neck has been observed to occur spontaneously in these systems, without the help of complex protein machinery as in biological cells. [25][26][27][28][29] Although not yet seen in experiments, particle-filled membrane tubes with narrow necks between the particles, see Fig. 1(c), have been predicted by numerical simulations.…”

Section: Introductionmentioning

confidence: 77%

Stabilization of membrane necks by adhesive particles, substrate surfaces, and constriction forces

Agudo-Canalejo

Lipowsky

2016

Soft Matter

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Membrane remodelling processes involving the formation and fission of small buds require the formation and closure of narrow membrane necks, both for biological membranes and for model membranes such as lipid bilayers. The conditions required for the stability of such necks are well understood in the context of budding of vesicles with bilayer asymmetry and/or intramembrane domains. In many cases, however, the necks form in the presence of an adhesive surface, such as a solid particle or substrate, or the cellular cortex itself. Examples of such processes in biological cells include endocytosis, exocytosis and phagocytosis of solid particles, the formation of extracellular and outer membrane vesicles by eukaryotic and prokaryotic cells, as well as the closure of the cleavage furrow in cytokinesis. Here, we study the interplay of curvature elasticity, membrane-substrate adhesion, and constriction forces to obtain generalized stability conditions for closed necks which we validate by numerical energy minimization. We then explore the consequences of these stability conditions in several experimentally accessible systems such as particle-filled membrane tubes, supported lipid bilayers, giant plasma membrane vesicles, bacterial outer membrane vesicles, and contractile rings around necks. At the end, we introduce an intrinsic engulfment force that directly describes the interplay between curvature elasticity and membrane-substrate adhesion.

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Section: Introductionmentioning

confidence: 77%

Stabilization of membrane necks by adhesive particles, substrate surfaces, and constriction forces

Agudo-Canalejo

Lipowsky

2016

Soft Matter

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“…However, lipid bilayers of 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) and 1,2‐dioleoyl‐sn‐glycero‐3‐phosphocholine (DOPC) are in fluidic phase at physiological temperature . Since, the first step in endocytic uptake is the deformation of cell membrane, and fluidic phase bilayers are more deformable and possess an order of magnitude lower bending modulus (8–10 × 10 −20 J) than gel phase bilayers (bending modulus:120 × 10 −20 J), it is reasonable to assume that phase transition of lipid bilayers may have an impact on the cellular uptake of liposomes and other nanomaterials. Therefore, in order to relate phase behavior of liposomes to cellular uptake, we prepared liposomes using DPPC, DMPC, and DOPC (Figure S1, Supporting Information) as the liposomes derived from these lipids have different phase transition temperatures.…”

Section: Resultsmentioning

confidence: 99%

Liposomal Treatment of Cancer Cells Modulates Uptake Pathway of Polymeric Nanoparticles by Altering Membrane Stiffness

Xiang

Sarem

Shah

et al. 2018

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Nanomedicines can be taken up by cells via nonspecific and dynamin-dependent (energy-dependent) clathrin and caveolae-mediated endocytosis. While significant effort has focused on targeting pathway-specific transporters, the role of nanobiophysics in the cell lipid bilayer nanoparticle uptake pathway remains largely unexplored. In this study, it is demonstrated that stiffness of lipid bilayer is a key determinant of uptake of liposomes by mammalian cells. Dynamin-mediated endocytosis (DME) of liposomes is found to correlate with its phase behavior, with transition toward solid phase promoting DME, and transition toward fluidic phase resulting in dynamin-independent endocytosis. Since liposomes can transfer lipids to cell membrane, it is sought to engineer the biophysical properties of the membrane of breast epithelial tumor cells (MD-MBA-231) by treatment with phosphatidylcholine liposomes, and elucidate its effect on the uptake of polymeric nanoparticles. Analysis of the giant plasma membrane vesicles derived from treated cells using flicker spectroscopy reveals that liposome treatment alters membrane stiffness and DME of nanoparticles. Since liposomes have a history of use in drug delivery, localized priming of tumors with liposomes may present a hitherto unexploited means of targeting tumors based on biophysical interactions.

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“…[5][6][7] To evaluate the risks of exposure to inhaled nanomaterials, recent experimental, theoretical and simulations studies have focused on the interaction of nanoparticles with unilamellar vesicles. [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] Depending on the particle size, several mechanisms have been proposed, leading to a wide variety of predictions including particles decorating the outer vesicle surface, supported lipid bilayers (SLB) or particles more generally engulfed in vesicles. 16,19,20,23,24 In the latter case, the fluid membrane invaginates and envelops one or several particles like in cellular endocytosis.…”

Section: -Introductionmentioning

confidence: 99%

“…[8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] Depending on the particle size, several mechanisms have been proposed, leading to a wide variety of predictions including particles decorating the outer vesicle surface, supported lipid bilayers (SLB) or particles more generally engulfed in vesicles. 16,19,20,23,24 In the latter case, the fluid membrane invaginates and envelops one or several particles like in cellular endocytosis. 16,21,22 SLBs on highly curved surfaces have been the focus of many recent studies, as they represent a means to coat particles with biocompatible interfaces.…”

Section: -Introductionmentioning

confidence: 99%

The role of surface charge in the interaction of nanoparticles with model pulmonary surfactants

Mousseau

Berret

2018

Soft Matter

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Inhaled nanoparticles traveling through the airways are able to reach the respiratory zone of the lungs. In such an event, the incoming particles first come into contact with the liquid lining the alveolar epithelium, the pulmonary surfactant. The pulmonary surfactant is composed of lipids and proteins that are assembled into large vesicular structures. The question of the nature of the biophysicochemical interaction with the pulmonary surfactant is central to understand how the nanoparticles can cross the air-blood barrier. Here we explore the phase behavior of sub-100 nm particles and surfactant substitutes under controlled conditions. Three types of surfactant mimetics, including the exogenous substitute Curosurf®, a drug administered to infants with respiratory distress syndrome, are tested together with aluminum oxide (Al2O3), silicon dioxide (SiO2) and polymer (latex) nanoparticles. The main result here is the observation of spontaneous nanoparticle-vesicle aggregation induced by coulombic attraction. The role of the surface charges is clearly established. We also evaluate the supported lipid bilayer formation recently predicted and find that in the cases studied these structures do not occur. Pertaining to the aggregate internal structure, fluorescence microscopy shows that the vesicles and particles are intermixed at the nano- to microscale. With particles acting as stickers between vesicles, it is anticipated that the presence of inhaled nanomaterials in the alveolar spaces could significantly modify the interfacial and bulk properties of the pulmonary surfactant and interfere with lung physiology.

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Inside Cover: Internalization of Silica Nanoparticles into Fluid Liposomes: Formation of Interesting Hybrid Colloids (Angew. Chem. Int. Ed. 46/2014)

Cited by 16 publications

References 0 publications

Stabilization of membrane necks by adhesive particles, substrate surfaces, and constriction forces

Stabilization of membrane necks by adhesive particles, substrate surfaces, and constriction forces

Liposomal Treatment of Cancer Cells Modulates Uptake Pathway of Polymeric Nanoparticles by Altering Membrane Stiffness

The role of surface charge in the interaction of nanoparticles with model pulmonary surfactants

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