Insight in the role of bovine serum albumin for promoting the in situ surface growth of polyhydroxybutyrate (PHB) on patterned surfaces via enzymatic surface-initiated polymerization

Niamsiri, Nuttawee; Bergkvist, Magnus; Delamarre, Soazig C.; Cady, Nathan; Coates, Geoffrey W.; Ober, Christopher K.; Batt, Carl A.

doi:10.1016/j.colsurfb.2007.05.023

Cited by 13 publications

(18 citation statements)

References 71 publications

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“…BSA probably has a great affinity for lignin and lignin containing substrates due to its highly hydrophobic nature, isoelectric point (pI), and formation of highly hydrophobic agglomerates at temperatures above about 508C (Echterhoff et al, 2001) and could coat exposed lignin to prevent unspecific, unproductive cellulase adsorption, thus making more enzyme available for hydrolysis (Willies, 2007;Yang and Wyman, 2006). Furthermore, BSA may enhance enzyme stability (Huang and Monk, 2004;Robert, 1983) and reduce the hydrophobicity of surfaces, facilitating cellulase adsorption and desorption (Niamsiri et al, 2007;Park et al, 2002;Tilton et al, 1991). However, the interaction of BSA with the substrate surface may be affected by temperature, pH, ionic strength, substrate hydrophobicity, and surface charge (Chandra et al, 2007;Echterhoff et al, 2001;Ha et al, 1993;Halder et al, 2005).…”

Section: Introductionmentioning

confidence: 96%

Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies

Kumar

Wyman²

2009

Biotech & Bioengineering

132

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Bovine serum albumin (BSA), Tween-20, and polyethylene glycol (PEG6000) were added to washed corn stover solids produced by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), dilute sulfuric acid (DA), lime, controlled pH, and sulfur dioxide (SO(2)) pretreatments and to untreated corn stover (UT) and pure Avicel glucan prior to adding cellulase supplemented with beta-glucosidase at an activity ratio of 1:2/g and a moderate enzyme loading of 16.1 mg/g glucan in the raw corn stover. The additives were applied individually at 150, 300, and 600 mg/g glucan in the pretreated solids and in combinations of equal amounts of each that totaled 600 mg/g. The greatest increase in total sugar release was by Tween-20 with SO(2) pretreated solids followed by PEG6000 with ARP solids and Tween-20 with lime solids. The effectiveness of the additives was observed to depend on the type of sugars left in the solids, suggesting that it may be more beneficial to use the mixture of these additives to realize a high total sugar yield. In addition, little enhancement in sugar release was possible beyond a loading of 150 mg additives/g glucan for most pretreatments, and combinations did not improve sugar release much over use of additives alone for all except SO(2). Additives were also found to significantly increase concentrations of cellobiose and cellooligomers after 72 h of Avicel hydrolysis.

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Section: Introductionmentioning

confidence: 96%

Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies

Kumar

Wyman²

2009

Biotech & Bioengineering

132

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“…However, this may not be the only explanation. As reported by Niamsiri et al, BSA can interact with and stabilize growing surface polymers 28 . We are currently investigating whether BSA can also interact with the growing DNA polymers and if this is crucial to the activity of TdT.…”

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confidence: 53%

“…However, no elongation of protruding DNA strands was observed from the recruited enzymes. It has previously been reported that enzymatic activity on surfaces is improved by the presence of BSA 28 . We therefore investigated the possibility of using BSA to promote nucleotide polymerization.…”

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confidence: 98%

Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

et al. 2015

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“…After ESIP investigations on PLA fibers, PHB granules were coated on and throughout the fibers with an average diameter of 350 nm (Figure 1). We also have found that the molecular weight (M w ), molecular number (M n ), and polydispersity index (PDI) of ESIP PHB on gold surfaces are 0.59 x 10 5 g/mol 1.06 x 10 5 g/mol and 1.8, respectively [15]. Table 1.…”

Section: Resultsmentioning

confidence: 84%

Piezoelectric Poly(3-hydroxybutyrate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue Engineering

Mendenhall

Frey

et al. 2007

MRS Proc.

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Three dimensional scaffolds (3D) are promising for future nanoscale materials and tissue engineering applications being that they have architecture and mechanical properties similar to natural tissue. In this work, poly(lactic acid) fibers were prepared via electrospinnig with average diameters of 2580 nm. Using Enzymatic Surface-Initiated polymerization (ESIP), poly(3hydroxybutyrate) were coated on poly(lactic acid) fibers. This provides an alternative method to enzymatic surface modification of fibers. ESIP of PHB produces a granular film providing surface topography and increases mechanical properties of PLA fibers alone. When using covalent approaches, PHB granules provide surface topography of 200-500 nm with a polydisperse coverage area. Compressive modulus measurements of PLA and PHB/PLA scaffolds were 25 kPa and 73 kPa, respectively. The percent crystallinity of PLA and PHB/PLA scaffolds was 17% and 30%, respectively. This rough topography, in addition to the crystallinity of the scaffold, facilitates Soas-2 osteoblast cell attachment. We have observed attachment of the osteoblast cells along the length of the oriented PLA and PHB/PLA composite scaffolds with different morphologies, rounded and stretched, throughout a depth of 90 µm.

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Insight in the role of bovine serum albumin for promoting the in situ surface growth of polyhydroxybutyrate (PHB) on patterned surfaces via enzymatic surface-initiated polymerization

Cited by 13 publications

References 71 publications

Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies

Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies

Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

Piezoelectric Poly(3-hydroxybutyrate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue Engineering

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