Insight into Buffalo (Bubalus bubalis) RIG1 and MDA5 Receptors: A Comparative Study on dsRNA Recognition and In-Vitro Antiviral Response

Singh, Mahender; Brahma, Biswajit; Maharana, Jitendra; Patra, Mahesh Chandra; Kumar, Sushil; Mishra, Purusottam; Saini, Megha; De, Bidhan Chandra; Mahanty, Sourav; Datta, Tirtha Kumar; De, Sachinandan

doi:10.1371/journal.pone.0089788

Cited by 6 publications

(7 citation statements)

References 51 publications

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“…Varying numbers (2 −8) of monomeric buCATHL4Bs peptides were initially placed asymmetrically in the aqueous phase close to one of the leaflets of the bilayer. All MD simulations were performed using the GROMACS 4.5 software package [ 19 ]. The Berger-lipid force field was used for phospholipids and Gromis96-53a6 parameters were used to represent the peptide interactions.…”

Section: Methodsmentioning

confidence: 99%

Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins

et al. 2015

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Cathelicidins are an ancient class of antimicrobial peptides (AMPs) with broad spectrum bactericidal activities. In this study, we investigated the diversity and biological activity of cathelicidins of buffalo, a species known for its disease resistance. A series of new homologs of cathelicidin4 (CATHL4), which were structurally diverse in their antimicrobial domain, was identified in buffalo. AMPs of newly identified buffalo CATHL4s (buCATHL4s) displayed potent antimicrobial activity against selected Gram positive (G+) and Gram negative (G-) bacteria. These peptides were prompt to disrupt the membrane integrity of bacteria and induced specific changes such as blebing, budding, and pore like structure formation on bacterial membrane. The peptides assumed different secondary structure conformations in aqueous and membrane-mimicking environments. Simulation studies suggested that the amphipathic design of buCATHL4 was crucial for water permeation following membrane disruption. A great diversity, broad-spectrum antimicrobial action, and ability to induce an inflammatory response indicated the pleiotropic role of cathelicidins in innate immunity of buffalo. This study suggests short buffalo cathelicidin peptides with potent bactericidal properties and low cytotoxicity have potential translational applications for the development of novel antibiotics and antimicrobial peptidomimetics.

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Section: Methodsmentioning

confidence: 99%

Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins

et al. 2015

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“…In the cytosol, retinoic acid-inducible gene 1 (RIG-1)-like helicases, including RIG-1 and melanoma differentiation-associated gene 5 (MDA5), detect double stranded viral RNA and play an important role in the regulation of interferon expression in response to viral infection ( 35 , 36 ). Work in embryonic chickens indicates that MDA5 is expressed in both the fetal spleen and lung from at least embryonic day 10 ( 35 ).…”

Section: Pattern-recognition Receptorsmentioning

confidence: 99%

“…Work in embryonic chickens indicates that MDA5 is expressed in both the fetal spleen and lung from at least embryonic day 10 ( 35 ). In studies utilizing primary fetal buffalo fibroblast cells, Poly I:C treatment resulted in significant increases in RIG-1, MDA-5, and interferon-β mRNA expression, suggesting that the presence of a functional innate immune response to simulated viral infection ( 36 ). RIG-1, MDA5, and interferon-β1 mRNA transcripts have also been shown to be up-regulated in primary human cord blood mast cells in response to stimulation with antibody enhanced dengue virus infection ( 37 ).…”

Section: Pattern-recognition Receptorsmentioning

confidence: 99%

Preterm Birth, Intrauterine Infection, and Fetal Inflammation

Kemp

2014

Front. Immunol.

153

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Preterm birth (PTB) (delivery before 37 weeks’ gestation) is a leading cause of neonatal death and disease in industrialized and developing countries alike. Infection (most notably in high-risk deliveries occurring before 28 weeks’ gestation) is hypothesized to initiate an intrauterine inflammatory response that plays a key role in the premature initiation of labor as well as a host of the pathologies associated with prematurity. As such, a better understanding of intrauterine inflammation in pregnancy is critical to our understanding of preterm labor and fetal injury, as well as on-going efforts to prevent PTB. Focusing on the fetal innate immune system responses to intrauterine infection, the present paper will review clinical and experimental studies to discuss the capacity for a fetal contribution to the intrauterine inflammation associated with PTB. Evidence from experimental studies to suggest that the fetus has the capacity to elicit a pro-inflammatory response to intrauterine infection is highlighted, with reference to the contribution of the lung, skin, and gastrointestinal tract. The paper will conclude that pathological intrauterine inflammation is a complex process that is modified by multiple factors including time, type of agonist, host genetics, and tissue.

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“…Primary culture of foetal skin derived fibroblast cells were prepared by a previously described method [ 23 ]. The cells were maintained in complete Dulbecco Modified Eagle’s medium (DMEM) supplemented with 10% FBS, 25mM HEPES, 10 ng/ml EGF, L-Glutamine (2 mM), Penicillin-G (10000 U/ml) and streptomycin (100 μg/ml).…”

Section: Methodsmentioning

confidence: 99%

Comparative Genomic Analysis of Buffalo (Bubalus bubalis) NOD1 and NOD2 Receptors and Their Functional Role in In-Vitro Cellular Immune Response

Brahma

Kumar

et al. 2015

PLoS ONE

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Nucleotide binding and oligomerization domain (NOD)-like receptors (NLRs) are innate immune receptors that recognize bacterial cell wall components and initiate host immune response. Structure and function of NLRs have been well studied in human and mice, but little information exists on genetic composition and role of these receptors in innate immune system of water buffalo—a species known for its exceptional disease resistance. Here, a comparative study on the functional domains of NOD1 and NOD2 was performed across different species. The NOD mediated in-vitro cellular responses were studied in buffalo peripheral blood mononuclear cells, resident macrophages, mammary epithelial, and fibroblast cells. Buffalo NOD1 (buNOD1) and buNOD2 showed conserved domain architectures as found in other mammals. The domains of buNOD1 and buNOD2 showed analogy in secondary and tertiary conformations. Constitutive expressions of NODs were ubiquitous in different tissues. Following treatment with NOD agonists, peripheral lymphocytes showed an IFN-γ response along-with production of pro-inflammatory cytokines. Alveolar macrophages and mammary epithelial cells showed NOD mediated in-vitro immune response through NF-κB dependent pathway. Fibroblasts showed pro-inflammatory cytokine response following agonist treatment. Our study demonstrates that both immune and non-immune cells could generate NOD-mediated responses to pathogens though the type and magnitude of response depend on the cell types. The structural basis of ligand recognition by buffalo NODs and knowledge of immune response by different cell types could be useful for development of non-infective innate immune modulators and next generation anti-inflammatory compounds.

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Insight into Buffalo (Bubalus bubalis) RIG1 and MDA5 Receptors: A Comparative Study on dsRNA Recognition and In-Vitro Antiviral Response

Cited by 6 publications

References 51 publications

Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins

Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins

Preterm Birth, Intrauterine Infection, and Fetal Inflammation

Comparative Genomic Analysis of Buffalo (Bubalus bubalis) NOD1 and NOD2 Receptors and Their Functional Role in In-Vitro Cellular Immune Response

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