Insight into Molecular Epidemiology, Antimicrobial Resistance, and Virulence Genes of Extensively Drug-Resistant <i>Acinetobacter baumannii</i> in Thailand

Kongthai, Phattaraporn; Thummeepak, Rapee; Leungtongkam, Udomluk; Pooarlai, Renuka; Kitti, Thawatchai; Thanwisai, Aunchalee; Chantratita, Narisara; Millard, Andrew; Sitthisak, Sutthirat

doi:10.1089/mdr.2020.0064

Cited by 19 publications

(18 citation statements)

References 42 publications

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“…Lee et al reported that the outbreak of XDR A. baumannii ST451 carrying MDR genes occurred in South Korea (24). Moreover, there have been outbreaks of A. baumannii ST451 in countries around China in recent years, such as Thailand and India (25)(26)(27), and ST451 was also isolated from patients with bloodstream infection, causing a certain mortality (28), so the prevalence of A. baumannii ST451 requires more attention. In order to understand the molecular characteristic and the transmission route of ST451 outbreak, one ST451 (Ab1) isolated from the environment and another ST451 (Ab2) from a patient in were selected for the genome sequence (Supplementary Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Analysis of Multidrug-Resistant Acinetobacter baumannii Isolates in a Tertiary Hospital Over a 12-Year Period in China

Jiang

Chen

Liu

et al. 2021

Front. Public Health

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Acinetobacter baumannii is an important nosocomial pathogen, which is multidrug resistant (MDR). Acinetobacter baumannii has become a major threat to public health worldwide due to its ability to easily acquire resistant genes. In order to analyze its epidemiology characteristics and the genetic evolution, A. baumannii isolates obtained from a Chinese tertiary hospital in the past 12 years (2008–2019), 295 isolates of non-repetitive A. baumannii, were recovered from patients and wards environments. The resistance genes were analyzed using antimicrobial susceptibility testing. The genetic relatedness of 295 isolates was identified by multilocus sequence typing (MLST) and eBURST analysis. It was found that the antibiotic-resistant and carbapenemase-resistant genes of all the 295 MDR A. baumannii in the hospital have not changed significantly over the past 12 years; all of them were resistant to multiple antibiotics except the polymyxin E and tigecycline. The results of drug-resistant genes showed that the detection rates of carbapenemase-resistant genes blaOXA−23, blaTEM−1, and blaOXA−66 were 97.6, 75.3, and 71.9%, respectively, which were detected almost every year from 2008 to 2019. Additionally, 16s rRNA methylation enzyme gene armA, aminoglycoside-resistant gene ant(3")-I, and class I integrase gene could also have a high positive rate. By MLST, these isolates were assigned to 12 sequence types (STs), including ST369, ST208, ST195, ST191, ST368, ST530, ST469, ST451, ST229, ST381, ST543, and ST1176. eBURST analysis showed that 9 STs with ST208 as the founder genotype belonged to Group 1 except for ST229, ST530, and ST1176. Therefore, most MDR A. baumannii isolates had a relatively close genetic relationship. Notably, the predominant ST208 and ST369 at the early stage changed to ST451 in 2019, indicating that the complex and diverse genetic background of the prevalence of A. baumannii isolates in the hospital. Overall, further epidemiological surveillance and genetic evolution analysis of A. baumannii are required, which can provide new strategies for the prevention and control of A. baumannii infections.

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Section: Discussionmentioning

confidence: 99%

Epidemiological Analysis of Multidrug-Resistant Acinetobacter baumannii Isolates in a Tertiary Hospital Over a 12-Year Period in China

Jiang

Chen

Liu

et al. 2021

Front. Public Health

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“…This strain is representative of the outbreak clone obtained from a hospital in east Thailand, and it was collected from November 2013 to February 2015 ( Leungtongkam et al, 2018a ). Antimicrobial susceptibility testing (AST) was performed as previously described ( Kongthai et al, 2021 ). The AST results were interpreted according to the Clinical Laboratory Standard Institute ( CLSI, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Then, the output was illustrated using R studio as described in https://github.com/IamIamI/pADAP_project/tree/master/Roary_stats . The average nucleotide identity (ANI) was calculated using FastANI v.1.3 to estimate the whole-genome similarity among the two XDRAB strains, which were obtained from hospitalized patients in Thailand ( Kongthai et al, 2021 ), and the five closest related genomes identified from the pan-genome analysis. Antimicrobial resistance and virulence genes were retrieved using the Comprehensive Antibiotic Resistance Database (CARD) and VFanalyzer ( Liu et al, 2019 ), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study of 339 A. baumannii isolates collected from four hospitals in Thailand revealed 7.9% XDRAB among the total isolates collected ( Leungtongkam et al, 2018a ). We found an outbreak clone of XDRAB in one hospital with the same ST type and plasmid group that belonged to IC2 ( Kongthai et al, 2021 ). All of them were ST98 (Pasteur) and contained GR2 and GR6 plasmids.…”

Section: Introductionmentioning

confidence: 97%

“…Plasmid classification of A. baumannii can be divided into 23 different groups (GR1- GR23) based on the nucleotide identity of the replicase genes ( repA ) using the polymerase chain reaction-based replicon typing (AB-PBRT) method ( Bertini et al, 2010 ; Salgado-Camargo et al, 2020 ). Kongthai et al (2021) reported the detection of tra genes in XDRAB strains harbored in plasmid GR6. This GR6 plasmid is responsible for the dissemination of drug resistance genes such as bla OXA-23 and aphA6 ( Saranathan et al, 2014 ; Leungtongkam et al, 2018a ).…”

Section: Introductionmentioning

confidence: 99%

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Insights into mobile genetic elements and the role of conjugative plasmid in transferring aminoglycoside resistance in extensively drug-resistant Acinetobacter baumannii AB329

Khongfak

Thummeepak

Leungtongkam

et al. 2022

PeerJ

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Acinetobacter baumannii is a major cause of nosocomial infection, and the incidence of extensively drug-resistant A. baumannii (XDRAB) infections has dramatically increased worldwide. In this study, we aimed to explore the complete genome sequence of XDRAB 329, ST1166/98 (Oxford/Pasteur), which is an outbreak clone from a hospital in Thailand. Whole-genome sequencing (WGS) was performed using short-read Illumina and long-read PacBio sequencing, and a conjugation assay of its plasmid was performed. The complete genome sequence of A. baumannii AB329 revealed a circular chromosome 3,948,038 bp in length with 39% GC content. Antibiotic resistance genes (ARGs), including beta-lactam resistance (blaOXA-51, blaADC-25, blaOXA-23, blaTEM-1D), aminoglycoside resistance (aph(3′)-Ia, aph(3″)-Ib, aph(6)-Id, armA), tetracycline resistance (tet(B), tet (R)), macrolide resistance (mph(E), msr(E)), and efflux pumps, were found. Mobile genetic elements (MGEs) analysis of A. baumannii AB329 revealed two plasmids (pAB329a and pAB329b), three prophages, 19 genomic islands (GIs), and 33 insertion sequences (ISs). pAB329a is a small circular plasmid of 8,731 bp, and pAB329b is a megaplasmid of 82,120 bp. aph(3′)-VIa was detected in pAB329b, and a major facilitator superfamily (MFS) transporter was detected in the prophage. Acinetobacter baumannii resistance island 4 (AbaR4) harboring tetracycline and aminoglycoside resistance was detected in the genome of A. baumannii AB329. pAB329b, which belongs to Rep-type GR6 (plasmid lineage LN_1), is a conjugative plasmid with the ability to transfer an aminoglycoside resistance gene to sodium azide-resistant A. baumannii. This study provides insights into the features of the MGEs of XDRAB, which are the main reservoir and source of dissemination of ARGs.

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Antimicrobial resistance and molecular epidemiology of virulence genes among multi-drug resistant Acinetobacter baumannii clinical isolates in Iran

et al. 2021

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Insight into Molecular Epidemiology, Antimicrobial Resistance, and Virulence Genes of Extensively Drug-Resistant Acinetobacter baumannii in Thailand

Cited by 19 publications

References 42 publications

Epidemiological Analysis of Multidrug-Resistant Acinetobacter baumannii Isolates in a Tertiary Hospital Over a 12-Year Period in China

Epidemiological Analysis of Multidrug-Resistant Acinetobacter baumannii Isolates in a Tertiary Hospital Over a 12-Year Period in China

Insights into mobile genetic elements and the role of conjugative plasmid in transferring aminoglycoside resistance in extensively drug-resistant Acinetobacter baumannii AB329

Antimicrobial resistance and molecular epidemiology of virulence genes among multi-drug resistant Acinetobacter baumannii clinical isolates in Iran

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