Insight into the metabolic mechanism of scoparone on biomarkers for inhibiting Yanghuang syndrome

Abstract

Scoparone (6,7-dimethoxycoumarin) is the representative ingredient of Yinchenhao (Artemisia capillaris Thunb.) which is a famous Chinese medicinal herb and shows favorable efficacy for all kinds of liver disease, specifically for the treatment of Yanghuang syndrome (YHS). The precise molecular mechanism concerning the action of scoparone on YHS is yet to be fully elucidated. The aim of the present study was to determine the mechanism of scoparone and evaluate its efficacy on metabolite levels. The differential expression of metabolites responsible for the pharmacological effects of scoparone was characterized and the protection effect of scoparone against this disease. Using multivariate statistical analysis, 33 biomarkers were identified using precise MS/MS and play an important role in the regulation of key metabolic pathways associated with liver disease. In addition, pathological results also showed consistent changes in the YHS model group and after treatment with scoparone, both the metabolic profile and histopathology resembled that of normal level, which suggesting favorable efficacy over the observed time period. The present work indicated that a metabolomics platform provided a new insight into understanding the mechanisms of action of natural medicines such as scoparone.

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

“…Scoparone (6,7‐dimethoxycoumarin) is a representative ingredient of Artemisia capillaris Thunb, which is a famous herbal medicine that was a positive effect on various liver diseases (Zhang et al, ; Liu & Zhao, ). Fang et al () studied the mechanism of scoparone and its efficacy on metabolic levels with MS‐based metabolomics. Differential metabolites produced by the pharmacological effects of scoparone were characterized and the therapeutic effects of scoparone on Yanghuang Syndrome.…”

Comparative metabolomics unveils molecular changes and metabolic networks of syringin against hepatitis B mice by untargeted mass spectrometry

“…[54][55][56][57][58][59] Liquid chromatography-mass spectrometry (LC-MS) is widely used in metabolic analysis. [60][61][62][63][64][65][66][67][68][69][70][71] It can monitor small molecule metabolites changes in biological samples. [72][73][74][75][76][77][78][79][80][81][82][83] In this study, we used the untargeted metabolomics technology to discover the metabolic pathways and biomarkers to reveal the potential biological mechanism of syringin on the hepatitis B virus.…”

Metabolomics biotechnology, applications, and future trends: a systematic review

“…Clearly, developments in chromatographic methods have been effective in separating metabolites and increasing the number of metabolites detected. 140,141 However, one major challenge is the inability to compare and correlate the results of these studies, which are performed on the same or similar samples obtained by independent research groups. This is the main bottleneck in the development of this eld.…”