Insight into the microbiology of nitrogen cycle in the dairy manure composting process revealed by combining high-throughput sequencing and quantitative PCR

Zhong, Xiao-Zhong; Zeng, Yan; Wang, Shi-Peng; Sun, Zhao‐Yong; Tang, Yue‐Qin; Kida, Kenji

doi:10.1016/j.biortech.2020.122760

Cited by 92 publications

(22 citation statements)

References 47 publications

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“…Previous studies have shown that stronger nitrification leads to the accumulation of NO 3 – -N ( Sun et al, 2019 ; Wan et al, 2020 ). Biological nitrification involves two steps: first, oxidizing NH 4 + -N to NO 2 – -N, and further oxidizing NO 2 – -N to NO 3 – -N ( Zhong et al, 2020 ). The reduction of NO 3 – -N was found on day 35 in TA and CK.…”

Section: Resultsmentioning

confidence: 99%

“…The reduction of NO 3 – -N was found on day 35 in TA and CK. This was because NO 3 – and NO 2 – shifted to NO, N 2 O through denitrification ( Zhong et al, 2020 ), and assimilation which promoted the generation of organic nitrogen. It is worth noting that ammonia oxidation occurs during the maturation phase of composting because nitrifying bacteria have limited survival at temperatures above 40°C ( Wan et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

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Different Effects of Thermophilic Microbiological Inoculation With and Without Biochar on Physicochemical Characteristics and Bacterial Communities in Pig Manure Composting

Sun

Long

et al. 2021

Front. Microbiol.

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This study evaluated the effects of thermophilic microbiological inoculation alone (TA) and integrated with biochar (TB) on the physicochemical characteristics and bacterial communities in pig manure (PM) composting with wheat straw. Both TA and TB accelerated the rate of temperature increase during the PM composting. TA significantly reduced total nitrogen loss by 18.03% as opposed to TB which significantly accelerated total organic carbon degradation by 12.21% compared with the control. Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria were the major phyla in composting. Variation of the relative abundance of genera depended on the composting period and treatment. The genera Lactobacillus (26.88–46.71%) and Clostridium_sensu_stricto (9.03–31.69%) occupied a superior position in the temperature rise stage, and Bacillus (30.90–36.19%) was outstanding in the cooling stage. Temperature, total nitrogen (TN), and ammonium nitrogen significantly influenced the bacterial phyla composition. TN, water content, and nitrite nitrogen were the main drivers of the bacterial community genera. Furthermore, our results demonstrated that microbiological consortia were resistant to high temperatures and could fix nitrogen for enriched Pseudomonas; however, when interacted with biochar, total organic carbon (TOC) degradation was accelerated for higher bacterial richness and diversity as well as overrepresented Corynebacterium.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Different Effects of Thermophilic Microbiological Inoculation With and Without Biochar on Physicochemical Characteristics and Bacterial Communities in Pig Manure Composting

Sun

Long

et al. 2021

Front. Microbiol.

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“…The reactors were turned over on days 1, 4, 8, 11, 21, 29, 39, 49, and 60 to encourage aeration and increase the oxygen content. Measurements were performed at the time of turning of each pile [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Microbial Inoculation on Carbon Preservation during Goat Manure Aerobic Composting

Wang

Gao

et al. 2021

Molecules

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Carbon is the crucial source of energy during aerobic composting. There are few studies that explore carbon preservation by inoculation with microbial agents during goat manure composting. Hence, this study inoculated three proportions of microbial agents to investigate the preservation of carbon during goat manure composting. The microbial inoculums were composed of Bacillus subtilis, Bacillus licheniformis, Trichoderma viride, Aspergillus niger, and yeast, and the proportions were B1 treatment (1:1:1:1:2), B2 treatment (2:2:1:1:2), and B3 treatment (3:3:1:1:2). The results showed that the contents of total organic carbon were enriched by 12.21%, 4.87%, and 1.90% in B1 treatment, B2 treatment, and B3 treatment, respectively. The total organic carbon contents of B1 treatment, B2 treatment, and B3 treatment were 402.00 ± 2.65, 366.33 ± 1.53, and 378.33 ± 2.08 g/kg, respectively. B1 treatment significantly increased the content of total organic carbon compared with the other two treatments (p < 0.05). Moreover, the ratio of 1:1:1:1:2 significantly reduced the moisture content, pH value, EC value, hemicellulose, and lignin contents (p < 0.05), and significantly increased the GI value and the content of humic acid carbon (p < 0.05). Consequently, the preservation of carbon might be a result not only of the enrichment of the humic acid carbon and the decomposition of hemicellulose and lignin, but also the increased OTU amount and Lactobacillus abundance. This result provided a ratio of microbial agents to preserve the carbon during goat manure aerobic composting.

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“…stercorarium is a strictly anaerobic, spore-forming, thermophilic (optimum growth temperature: 65°C), and rod-shaped bacteria with peritrichous flagella that was first isolated from a compost pile in 1983 [109]. Numerous studies indicate that C. stercorarium plays a highly active role in the degradation of plant biomass in anaerobic, thermophilic digesters, highlighting its potential for CBP [111][112][113][114][115]. In 2001 two other bacteria with different flagella arrangements and carbon source utilization , namely Thermobacteroides leptospartum [116] and Clostridium thermolacticum [117] were found to be closely related enough to C. stercorarium to be considered subspecies, necessitating the new name change of the type-species (strain DSMZ 8532) to C. stercorarium subsp.…”

Section: Clostridium Stercorariumthermophilic Cellulosic/hemicellulosic Cbp Candidatementioning

confidence: 99%

Digestibility of Wheat and Cattail Biomass Using a Co-culture of Thermophilic Anaerobes for Consolidated Bioprocessing

et al. 2020

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Alternative low-carbon transportation fuels, such as biofuels, are needed to replace or supplement fossil fuels in order to lower global greenhouse gas emissions and combat climate change. Lignocellulosic biofuels have relatively low carbon emissions and are created using the non-food parts of crops and other plants, such as the leaves and stems, which are comprised mostly of a tough material called lignocellulose, composed of cellulose, hemicellulose, and lignin. One of the best lignocellulose degraders found in nature that is also capable of fermenting the released sugars to ethanol is Clostridium thermocellum, although improvements in both lignocellulose hydrolysis extent and ethanol yields are needed for commercial viability.C. thermocellum, considered a cellulose-degrading specialist, was co-cultured with two different hemicellulose-specialists, C. stercorarium and Thermoanaerobacter thermohydrosulfuricus. The hypothesis was that the co-cultures might degrade more lignocellulose owing to the additional hydrolytic enzymes supplied by the partners and their ability to uptake the inhibitory hemicellulose sugars. All co-culture combinations were found to solubilize more wheat straw, among other lignocellulose materials, and produce more endproducts, including ethanol, than C. thermocellum alone. These co-cultures were stable over multiple serial passages, on either wheat straw or pure cellulose, although some evidence of carbon competition was observed. The tri-culture was successfully used to screen the digestibility of various lignocellulose materials, revealing substantial difference between cattail harvested in different seasons. Cross-feeding of vital growth factors was observed between the various co-culture members in a defined medium.The metabolism of C. thermocellum is atypical compared to many organisms, including the absence of a pyruvate kinase, and its substitution with both a malate shunt and a putative Thank you to my friends and family for always supporting me along this journey. I would like to thank my supervisor, Dr. Richard Sparling, for giving me this opportunity and for his incredibly valuable guidance and mentorship, the strict scientific rigour helped me develop my critical thinking skills.

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Insight into the microbiology of nitrogen cycle in the dairy manure composting process revealed by combining high-throughput sequencing and quantitative PCR

Cited by 92 publications

References 47 publications

Different Effects of Thermophilic Microbiological Inoculation With and Without Biochar on Physicochemical Characteristics and Bacterial Communities in Pig Manure Composting

Different Effects of Thermophilic Microbiological Inoculation With and Without Biochar on Physicochemical Characteristics and Bacterial Communities in Pig Manure Composting

Effect of Microbial Inoculation on Carbon Preservation during Goat Manure Aerobic Composting

Digestibility of Wheat and Cattail Biomass Using a Co-culture of Thermophilic Anaerobes for Consolidated Bioprocessing

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