Insights into aluminum-tolerance pathways in Stylosanthes as revealed by RNA-Seq analysis

Jiang, Caode; Liu, Lusheng; Li, Xiaofeng; Han, Ruizhi; Wei, Yuansong; Yu, Yang

doi:10.1038/s41598-018-24536-3

“…DAG, Ca 2+ and protein kinases [9]. Proteins associated with the G-protein-mediated signaling pathway, DGAT and kinase cascades (CPK1 and CKI) were detected in the present study (S1, S2 and S8 Tables).…”

Section: Plos Onesupporting

confidence: 57%

“…For decades, a number of proteins contributing to Al 3+ tolerance have been identified in plants, and many studies have provided direct evidence linking Al 3+ -induced OA exudation from plant roots to malate and citrate efflux transporters (ALMTs), multidrug and toxic compound extrusion proteins (MATEs) and H + -ATPase activity in the plasma membrane [ 1 , 8 ]. In Stylosanthes roots, our recent data revealed that the signaling cascades of Al 3+ -induced citrate exudation comprise heterotrimeric G-proteins, phosphoinositide phospholipase C (PLC), inositol triphosphate (IP 3 ), diacylglycerol (DAG), Ca 2+ and protein kinases [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative phosphoproteomic analysis provides insights into the aluminum-responsiveness of Tamba black soybean

Han

¹

,

Wei

²

,

Xie

³

et al. 2020

PLoS ONE

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Aluminum (Al 3+) toxicity is one of the most important limitations to agricultural production worldwide. The overall response of plants to Al 3+ stress has been documented, but the contribution of protein phosphorylation to Al 3+ detoxicity and tolerance in plants is unclear. Using a combination of tandem mass tag (TMT) labeling, immobilized metal affinity chromatography (IMAC) enrichment and liquid chromatography-tandem mass spectrometry (LC-MS/MS), Al 3+-induced phosphoproteomic changes in roots of Tamba black soybean (TBS) were investigated in this study. The Data collected in this study are available via ProteomeXchange with the identifier PXD019807. After the Al 3+ treatment, 189 proteins harboring 278 phosphosites were significantly changed (fold change > 1.2 or < 0.83, p < 0.05), with 88 upregulated, 96 downregulated and 5 up-/downregulated. Enrichment and protein interaction analyses revealed that differentially phosphorylated proteins (DPPs) under the Al 3+ treatment were mainly related to G-protein-mediated signaling, transcription and translation, transporters and carbohydrate metabolism. Particularly, DPPs associated with root growth inhibition or citric acid synthesis were identified. The results of this study provide novel insights into the molecular mechanisms of TBS post-translational modifications in response to Al 3+ stress.

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“…Recently, transcriptome and proteome profiles have been used to analyze the responses of stylo to Al and Mn toxicity [47][48][49]. However, metabolic alterations of stylo roots in response to P deficiency have yet to be reported.…”

mentioning

confidence: 99%

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

Luo

¹

,

Liu

²

,

Zhang

³

et al. 2019

Preprint

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Background: Phosphorus (P) deficiency is one of the major constraints limiting plant growth, especially in acid soils. Stylosanthes (stylo) is a pioneer tropical legume with excellent adaptability to low P stress, but its underlying mechanisms remain largely unknown. Results: In this study, the physiological, molecular and metabolic changes in stylo responding to phosphate (Pi) starvation were investigated. Under low P condition, the root growth in stylo was significantly enhanced, which was accompanied with up-regulation of expansin genes participating in cell wall loosening. Metabolic profiling analysis showed that a total of 256 metabolites with differential accumulation were identified in stylo roots responding to P deficiency, which mainly include flavonoids, sugars, nucleotides, amino acids, phenylpropanoids and phenylamides. P deficiency led to significant reduction in the accumulation of phosphorylated metabolites (e.g., P-containing sugars, nucleotides and cholines), suggesting that internal P utilization was enhanced in stylo roots. However, flavonoid metabolites, such as kaempferol, daidzein and their glycoside derivatives, were significantly increased in P-deficient stylo roots. Furthermore, the transcripts of various genes involved in flavonoids synthesis were found to be up-regulated by Pi starvation in stylo roots. In addition, the abundance of phenolic acids and phenylamides was significantly increased in stylo roots during P deficiency. The enhanced accumulation of the metabolites in stylo roots, such as flavonoids, phenolic acids and phenylamides, might facilitate P solubilization and cooperate with beneficial microorganisms in rhizosphere, and thus contributing to P acquisition and utilization in stylo. Conclusions: These results suggest that stylo plants cope with P deficiency by modulating root morphology, scavenging internal Pi from phosphorylated metabolites and enhancing accumulation of flavonoids, phenolic acids and phenylamides. This study provides valuable insights into the complex responses and adaptive mechanisms of stylo to P deficiency.

show abstract

“…and Mn toxicity [47][48][49]. However, metabolic alterations of stylo roots in response to P deficiency have yet to be reported.…”

Section: Recently Transcriptome and Proteome Profiles Have Been Usedmentioning

confidence: 99%

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

Luo

¹

,

Liu

²

,

Zhang

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Background: Phosphorus (P) deficiency is one of the major constraints limiting plant growth, especially in acid soils. Stylosanthes (stylo) is a pioneer tropical legume with excellent adaptability to low P stress, but its underlying mechanisms remain largely unknown. Results: In this study, the physiological, molecular and metabolic changes in stylo responding to phosphate (Pi) starvation were investigated. Under low P condition, the root growth in stylo was significantly enhanced, which was accompanied with up-regulation of expansin genes participating in cell wall loosening. Metabolic profiling analysis showed that a total of 256 metabolites with differential accumulation were identified in stylo roots responding to P deficiency, which mainly include flavonoids, sugars, nucleotides, amino acids, phenylpropanoids and phenylamides. P deficiency led to significant reduction in the accumulation of phosphorylated metabolites (e.g., P-containing sugars, nucleotides and cholines), suggesting that internal P utilization was enhanced in stylo roots. However, flavonoid metabolites, such as kaempferol, daidzein and their glycoside derivatives, were significantly increased in P-deficient stylo roots. Furthermore, the transcripts of various genes involved in flavonoids synthesis were found to be up-regulated by Pi starvation in stylo roots. In addition, the abundance of phenolic acids and phenylamides was significantly increased in stylo roots during P deficiency. The enhanced accumulation of the metabolites in stylo roots, such as flavonoids, phenolic acids and phenylamides, might facilitate P solubilization and cooperate with beneficial microorganisms in rhizosphere, and thus contributing to P acquisition and utilization in stylo. Conclusions: These results suggest that stylo plants cope with P deficiency by modulating root morphology, scavenging internal Pi from phosphorylated metabolites and enhancing accumulation of flavonoids, phenolic acids and phenylamides. This study provides valuable insights into the complex responses and adaptive mechanisms of stylo to P deficiency.

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Insights into aluminum-tolerance pathways in Stylosanthes as revealed by RNA-Seq analysis

Cited by 33 publications

References 29 publications

Quantitative phosphoproteomic analysis provides insights into the aluminum-responsiveness of Tamba black soybean

Quantitative phosphoproteomic analysis provides insights into the aluminum-responsiveness of Tamba black soybean

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

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