Insights into epidemiology of human parvovirus B19 and detection of an unusual genotype 2 variant, Bulgaria, 2004 to 2013

Ivanova, Stefka; Mihneva, Zafira; Toshev, A.; Kovaleva, Valentina; Andonova, L.; Hübschen, Judith M.

doi:10.2807/1560-7917.es.2016.21.4.30116

Cited by 16 publications

(11 citation statements)

References 32 publications

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“…In some cases these rashes may have typical ways of appearing, spreading, and fading, and a certain distribution, which supports clinical diagnosis [8]. Nevertheless, laboratory confirmation of measles is essential, especially in measles elimination settings [9], when the majority of clinically suspected cases are caused by other aetiologies [10,11].…”

Section: Clinical and Differential Diagnosismentioning

confidence: 92%

Challenges of measles and rubella laboratory diagnostic in the era of elimination

Hübschen

Bork

Brown

et al. 2017

Clinical Microbiology and Infection

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The Member States of the WHO European Region adopted the goal of measles and rubella elimination more than 10 years ago, but so far only 21 of 53 countries have reached this target. Laboratory investigation of suspected cases is essential to support disease elimination efforts. Therefore, WHO maintains a network of accredited laboratories providing high-quality testing. Laboratory investigation heavily relies on specific IgM serology and increasingly on virus detection by reverse transcription (RT)-PCR, but other methods such as IgG avidity testing and genetic characterization of virus strains have gained in importance. In elimination settings, often few samples from suspected cases are available for testing, but testing proficiency must be maintained. The predictive value of an IgM-positive result decreases and other rash-fever disease aetiologies become more important. In addition, cases with a rash after measles/rubella vaccination or with mild disease after waning of vaccine-induced antibodies are seen more often. Thus, it is necessary to perform comprehensive and potentially time-consuming and costly investigations of every suspected case using quality-controlled laboratory methods. At the same time rapid feedback to public health officers is required for timely interventions. The introduction of new laboratory methods for comprehensive case investigations requires training of staff under the supervision of WHO-accredited reference laboratories and the definition of appropriate test algorithms. Clinical, laboratory, and epidemiological data are essential for final case classification and investigation of chains of transmission in the endgame of measles and rubella elimination.

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Section: Clinical and Differential Diagnosismentioning

confidence: 92%

Challenges of measles and rubella laboratory diagnostic in the era of elimination

Hübschen

Bork

Brown

et al. 2017

Clinical Microbiology and Infection

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“…Of the three B19V genotypes, by far the most common genotype currently circulating in the population worldwide is genotype 1 (343). Since the 1960s, genotype 2 has been seldom found in acute infections or in blood (58,258,(343)(344)(345)(346)(347)(348). However, due to the ability of the B19V genome to persist in tissues for decades after infection (58, 200-204) (see "B19V infection in other tissues," above), it can frequently be detected within various soft tissues of subjects born before 1972 (58).…”

Section: Genotypes and Molecular Epidemiologymentioning

confidence: 99%

Human Parvoviruses

2017

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Parvovirus B19 (B19V) and human bocavirus 1 (HBoV1), members of the large Parvoviridae family, are human pathogens responsible for a variety of diseases. For B19V in particular, host features determine disease manifestations. These viruses are prevalent worldwide and are culturable in vitro, and serological and molecular assays are available but require careful interpretation of results. Additional human parvoviruses, including HBoV2 to -4, human parvovirus 4 (PARV4), and human bufavirus (BuV) are also reviewed. The full spectrum of parvovirus disease in humans has yet to be established. Candidate recombinant B19V vaccines have been developed but may not be commercially feasible. We review relevant features of the molecular and cellular biology of these viruses, and the human immune response that they elicit, which have allowed a deep understanding of pathophysiology.

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“…B19V‐IgM positive and equivocal samples were investigated by polymerase chain reaction (PCR) and the products were sequenced and genotyped as described previously, except that MEGA7 was used for phylogenetic analysis.…”

Section: Introductionmentioning

confidence: 99%

Identification of human parvovirus B19 among measles and rubella suspected patients from Cuba

Ribas

Tejero

Cordero

et al. 2019

Journal of Medical Virology

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Between September 2014 and December 2015, 298 sera from rash and fever patients from all over Cuba were investigated for specific IgM antibodies against measles, rubella, dengue, human parvovirus B19 (B19V) and human herpesvirus 6 (HHV6) using a commercial enzyme-linked immunosorbent assay kits. B19V IgM positive and equivocal samples were investigated by a polymerase chain reaction and genotyping. No measles, rubella or dengue cases were detected. HHV6-IgM antibodies were confirmed in 5.7% and B19V-IgM antibodies in 10.7% of the patients. A total of 31.3% of the B19V cases were between 5 and 9 years old and 34.4% were 20 years and older. The only B19V sequence obtained belonged to genotype 1a. Diagnosis was established for only 16% of the rash and fever patients, suggesting that other diseases such as Zika or Chikungunya may play a role. K E Y W O R D SCuba, human parvovirus B19, rash-fever

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Insights into epidemiology of human parvovirus B19 and detection of an unusual genotype 2 variant, Bulgaria, 2004 to 2013

Cited by 16 publications

References 32 publications

Challenges of measles and rubella laboratory diagnostic in the era of elimination

Challenges of measles and rubella laboratory diagnostic in the era of elimination

Human Parvoviruses

Identification of human parvovirus B19 among measles and rubella suspected patients from Cuba

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