Insights into kisspeptin- and leptin-signalling on GnRH mRNA expression in hypothalamic organ cultures of immature pikeperch Sander lucioperca

Schaefer, Fabian; Wuertz, Sven

doi:10.1007/s40071-016-0134-1

Cited by 7 publications

(2 citation statements)

References 32 publications

(37 reference statements)

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“…The cart -dependent regulation of gnrh secretion by activated leptin signal had been already reported in rat 71,72 . Also, in a Perciforme species, it has been shown that leptin increases the brain expression of gnrh2 , but not gnrh1 73 . Our results indicate that the functional leptin signal induces the expression of anorexigenic gnrh2 in zebrafish, in normal feeding condition.…”

Section: Discussionmentioning

confidence: 96%

Transcriptional study of appetite regulating genes in the brain of zebrafish (Danio rerio) with impaired leptin signalling

Ahi

Brunel

Tsakoumis

et al. 2019

Sci Rep

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The hormone leptin is a key regulator of body weight, food intake and metabolism. In mammals, leptin acts as an anorexigen and inhibits food intake centrally by affecting the appetite centres in the hypothalamus. In teleost fish, the regulatory connections between leptin and other appetite-regulating genes are largely unknown. In the present study, we used a zebrafish mutant with a loss of function leptin receptor to investigate brain expression patterns of 12 orexigenic and 24 anorexigenic genes under different feeding conditions (normal feeding, 7-day fasting, 2 and 6-hours refeeding). Expression patterns were compared to wild-type zebrafish, in order to identify leptin-dependent differentially expressed genes under different feeding conditions. We provide evidence that the transcription of certain orexigenic and anorexigenic genes is influenced by leptin signalling in the zebrafish brain. We found that the expression of orexigenic genes was not affected by impaired leptin signalling under normal feeding conditions; however, several orexigenic genes showed increased transcription during fasting and refeeding, including agrp, apln, galr1a and cnr1. This suggests an inhibitory effect of leptin signal on the transcription of these orexigenic genes during short-term fasting and refeeding in functional zebrafish. Most pronounced effects were observed in the group of anorexigenic genes, where the impairment of leptin signalling resulted in reduced gene expression in several genes, including cart family, crhb, gnrh2, mc4r, pomc and spx, in the control group. This suggests a stimulatory effect of leptin signal on the transcription of these anorexigenic genes under normal feeding condition. In addition, we found multiple gain and loss in expression correlations between the appetite-regulating genes, in zebrafish with impaired leptin signal, suggesting the presence of gene regulatory networks downstream of leptin signal in zebrafish brain. The results provide the first evidence for the effects of leptin signal on the transcription of various appetite-regulating genes in zebrafish brain, under different feeding conditions. Altogether, these transcriptional changes suggest an anorexigenic role for leptin signal, which is likely to be mediated through distinct set of appetite-regulating genes under different feeding conditions.

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Section: Discussionmentioning

confidence: 96%

Transcriptional study of appetite regulating genes in the brain of zebrafish (Danio rerio) with impaired leptin signalling

Ahi

Brunel

Tsakoumis

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Similarly, the cart -dependent regulation of Gnrh secretion by activated leptin signal had been demonstrated in rat brain as well (Lebrethon et al 2000 ; Parent et al 2000 ). Moreover, in Sander lucioperca , a perciform fish species, it is shown that leptin induces the brain expression of gnrh2 (Schaefer and Wuertz 2016 ), and in goldfish, gnrh2 has been reported to be a downstream transcriptional target of crh (Kang et al 2011 ). In response to feeding, crh and gnrh2 have been recently reported to have similar expression patterns in Schizothorax davidi (another Cypriniformes species) (Yuan et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain

Ahi

Tsakoumis

Brunel

et al. 2021

Fish Physiol Biochem

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The signal mediated by leptin hormone and its receptor is a major regulator of body weight, food intake and metabolism. In mammals and many teleost fish species, leptin has an anorexigenic role and inhibits food intake by influencing the appetite centres in the hypothalamus. However, the regulatory connections between leptin and downstream genes mediating its appetite-regulating effects are still not fully explored in teleost fish. In this study, we used a loss of function leptin receptor zebrafish mutant and real-time quantitative PCR to assess brain expression patterns of several previously identified anorexigenic genes downstream of leptin signal under different feeding conditions (normal feeding, 7-day fasting, 2 and 6-h refeeding). These downstream factors include members of cart genes, crhb and gnrh2, as well as selected genes co-expressed with them based on a zebrafish co-expression database. Here, we found a potential gene expression network (GRN) comprising the abovementioned genes by a stepwise approach of identifying co-expression modules and predicting their upstream regulators. Among the transcription factors (TFs) predicted as potential upstream regulators of this GRN, we found expression pattern of sp3a to be correlated with transcriptional changes of the downstream gene network. Interestingly, the expression and transcriptional activity of Sp3 orthologous gene in mammals have already been implicated to be under the influence of leptin signal. These findings suggest a potentially conserved regulatory connection between leptin and sp3a, which is predicted to act as a transcriptional driver of a downstream gene network in the zebrafish brain.

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Trajectory of leptin and leptin receptor in vertebrates: Structure, function and their regulation

Bakshi

Singh

Rai

2022

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Insights into kisspeptin- and leptin-signalling on GnRH mRNA expression in hypothalamic organ cultures of immature pikeperch Sander lucioperca

Cited by 7 publications

References 32 publications

Transcriptional study of appetite regulating genes in the brain of zebrafish (Danio rerio) with impaired leptin signalling

Transcriptional study of appetite regulating genes in the brain of zebrafish (Danio rerio) with impaired leptin signalling

Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain

Trajectory of leptin and leptin receptor in vertebrates: Structure, function and their regulation

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