Insights into the chicken bursa of fabricius response to Newcastle disease virus at 48 and 72 hours post-infection through RNA-seq

Wang, Xiangwei; Jia, Yanqing; Ren, Juan; Liu, Haijin; Adam, FathalrhmanEisa Addoma; Yang, Zhicong

doi:10.1016/j.vetmic.2019.108389

Cited by 13 publications

(11 citation statements)

References 26 publications

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“…According to the KEGG mapping, in total, 36 metabolic pathways were significantly enriched ( p < 0.05) with 7 at 24 hpi and 29 at 36 hpi when comparing NA-1 to LaSota ( Figure 4 C,D). With the progress of a highly virulent NDV propagation, the numbers of the reprogrammed cellular metabolic pathways increased; therefore, the results presented here are in line with previous studies [ 38 , 40 ].…”

Section: Resultssupporting

confidence: 92%

Intense Innate Immune Responses and Severe Metabolic Disorders in Chicken Embryonic Visceral Tissues Caused by Infection with Highly Virulent Newcastle Disease Virus Compared to the Avirulent Virus: A Bioinformatics Analysis

Cheng

Liu

et al. 2022

Viruses

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The highly virulent Newcastle disease virus (NDV) isolates typically result in severe systemic pathological changes and high mortality in Newcastle disease (ND) illness, whereas avirulent or low-virulence NDV strains can cause subclinical disease with no morbidity and even asymptomatic infections in birds. However, understanding the host’s innate immune responses to infection with either a highly virulent strain or an avirulent strain, and how this response may contribute to severe pathological damages and even mortality upon infection with the highly virulent strain, remain limited. Therefore, the differences in epigenetic and pathogenesis mechanisms between the highly virulent and avirulent strains were explored, by transcriptional profiling of chicken embryonic visceral tissues (CEVT), infected with either the highly virulent NA-1 strain or the avirulent vaccine LaSota strain using RNA-seq. In our current paper, severe systemic pathological changes and high mortality were only observed in chicken embryos infected with the highly virulent NA-1 strains, although the propagation of viruses exhibited no differences between NA-1 and LaSota. Furthermore, virulent NA-1 infection caused intense innate immune responses and severe metabolic disorders in chicken EVT at 36 h post-infection (hpi), instead of 24 hpi, based on the bioinformatics analysis results for the differentially expressed genes (DEGs) between NA-1 and LaSota groups. Notably, an acute hyperinflammatory response, characterized by upregulated inflammatory cytokines, an uncontrolled host immune defense with dysregulated innate immune response-related signaling pathways, as well as severe metabolic disorders with the reorganization of host–cell metabolism were involved in the host defense response to the CEVT infected with the highly virulent NA-1 strain compared to the avirulent vaccine LaSota strain. Taken together, these results indicate that not only the host’s uncontrolled immune response itself, but also the metabolic disorders with viruses hijacking host cell metabolism, may contribute to the pathogenesis of the highly virulent strain in ovo.

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Section: Resultssupporting

confidence: 92%

Intense Innate Immune Responses and Severe Metabolic Disorders in Chicken Embryonic Visceral Tissues Caused by Infection with Highly Virulent Newcastle Disease Virus Compared to the Avirulent Virus: A Bioinformatics Analysis

Cheng

Liu

et al. 2022

Viruses

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“… , the allantoic cavity infection) or bursa of fabricius ( Fig. 2B , Intramuscular injection) RNA sequencing, it was found that the expression of IFI35 was significantly increased [ 10 , 22 ]. In healthy SPF chickens, ch IFI35 mRNA had high level transcription in the lungs, glandular stomach, brain, bursa of fabricius, gizzard, kidney, heart, intestines, liver, and muscle, whereas, lower level expressed in the trachea, spleen, pancreas, and skin ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of chicken <i>IFI35</i> and its antiviral activity against Newcastle disease virus

Jia¹,

WANG

Chen

et al. 2022

The Journal of Veterinary Medical Science

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Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by IFN-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading frame (ORF) of 1,152 bp encoding a protein of 384 amino acids containing two conserved Nmi/IFI35 domain (NID) motifs. Tissue distribution analysis of chIFI35 in healthy and Newcastle disease (ND) virus-infected chickens indicated a positive correlation between chIFI35 mRNA transcription and ND viral loads in various tissues. The role of chIFI35 in regulation NDV replication were further assessed by up-or down-regulated chIFI35 expression in DF-1 cells transfected with plasmid harboring chIFI35, pCMV-3HA-chIFI35 or shRNA targeting chIFI35, pshRNA-chIFI35 plasmids. NDV replications in DF-1 cells were significantly reduced or slightly increased by over-or under-expression of the chIFI35 protein, respectively, indicating the role of chIFI35 in anti-NDV infection. Moreover, chIFI35 also involved in regulation of viral gene transcription and IFNs expression. The collected data were meaningful for research chicken antiviral immunity and shed light on the pleiotropic antiviral effect of chIFI35 during NDV infection.

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“…At present, FM by fasting is increasingly widely applied, but its genetic mechanism has not been elucidated. With the development of next-generation sequencing technology, the transcriptome is highly accurate in the detection of differentially expressed genes (DEGs) for important economic traits in chickens, such as growth and development [22][23][24][25], reproduction traits [26][27][28][29], and disease-resistance traits [30][31][32][33]. However, a transcriptome study of FM has not yet been conducted in chickens.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting

Zhang

Chen

Wen

et al. 2021

Genes

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Molting in birds provides us with an ideal genetic model for understanding aging and rejuvenation since birds present younger characteristics for reproduction and appearance after molting. Forced molting (FM) by fasting in chickens causes aging of their reproductive system and then promotes cell redevelopment by providing water and feed again. To reveal the genetic mechanism of rejuvenation, we detected blood hormone indexes and gene expression levels in the hypothalamus and ovary of hens from five different periods during FM. Three hormones were identified as participating in FM. Furthermore, the variation trends of gene expression levels in the hypothalamus and ovary at five different stages were found to be basically similar using transcriptome analysis. Among them, 45 genes were found to regulate cell aging during fasting stress and 12 genes were found to promote cell development during the recovery period in the hypothalamus. In addition, five hub genes (INO80D, HELZ, AGO4, ROCK2, and RFX7) were identified by WGCNA. FM can restart the reproductive function of aged hens by regulating expression levels of genes associated with aging and development. Our study not only enriches the theoretical basis of FM but also provides insights for the study of antiaging in humans and the conception mechanism in elderly women.

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Insights into the chicken bursa of fabricius response to Newcastle disease virus at 48 and 72 hours post-infection through RNA-seq

Cited by 13 publications

References 26 publications

Intense Innate Immune Responses and Severe Metabolic Disorders in Chicken Embryonic Visceral Tissues Caused by Infection with Highly Virulent Newcastle Disease Virus Compared to the Avirulent Virus: A Bioinformatics Analysis

Intense Innate Immune Responses and Severe Metabolic Disorders in Chicken Embryonic Visceral Tissues Caused by Infection with Highly Virulent Newcastle Disease Virus Compared to the Avirulent Virus: A Bioinformatics Analysis

Characterization of chicken <i>IFI35</i> and its antiviral activity against Newcastle disease virus

Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting

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