Insights into the mechanism of copper-tolerance in Fibroporia radiculosa: The biosynthesis of oxalate

Ohno, Katie M.; Clausen, Carol A.; Green, Frederick; Diehl, Susan V.

doi:10.1016/j.ibiod.2015.08.016

Cited by 18 publications

(14 citation statements)

References 23 publications

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“…Among F. radiculosa isolates, there is diversity in timing and levels of gene expression. Differences in F. radiculosa isolates seen in this study were consistent with previous work suggesting F. radiculosa L-9414-SP has a delayed response in adapting to copper when compared to other F. radiculosa isolates (Jenkins, 2012;Ohno et al, 2015). Additional isolates of F. radiculosa (TFFH 294 and L-11659-SP) have been documented to have differences in oxalate accumulation and differential expression of genes encoding a citrate synthase, an isocitrate lyase, a glyoxylate dehydrogenase, a succinate/fumarate antiporter, and a copper transporting ATPase pump over the course of 8 weeks (Ohno et al, 2015).…”

supporting

confidence: 91%

“…It is hypothesized that high extracellular oxalic acid accumulation aids in the precipitation of copper ions into insoluble copper oxalate crystals, inactivating copper in this process (Gadd, 1993;DeGroot and Woodward, 1999;Green and Clausen, 2003;Hastrup et al, 2005;Freeman and McIntyre, 2008). Additionally, it has been documented that copper-tolerant fungi produce two to 17 times more oxalic acid when exposed to coppertreated wood compared to non-treated wood (Green and Clausen, 2005;Jenkins, 2012;Ohno et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression Analysis of Three Putative Copper-Transporting ATPases in Copper-Tolerant Fibroporia radiculosa

2020

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Copper tolerance of brown-rot basidiomycete decay fungi can lessen the efficacy of copper-containing wood preservatives for wood products in-service. The purpose of this study was to evaluate wood mass loss and differential expression of three genes that have putative annotations for copper-transporting ATPase pumps (FIBRA_00974, FIBRA_04716, and FIBRA_01430). Untreated southern pine (SP) and SP treated with three concentrations of ammoniacal copper citrate (CC, 0.6, 1.2, and 2.4%) were exposed to two copper-tolerant Fibroporia radiculosa isolates (FP-90848-T and L-9414-SP) and copper-sensitive Gloeophyllum trabeum isolate (MAD 617) in a 4-week-long standard decay test (AWPA E10-19). Decay of copper-treated wood was inhibited by G. trabeum (p = 0.001); however, there was no inhibition of decay with increasing copper concentrations by both F. radiculosa isolates. Initially, G. trabeum and one F. radiculosa isolate (L-9414-SP) highly upregulated FIBRA_00974 and FIBRA_04716 on copper-treated wood at week 1 (p = 0.005), but subsequent expression was either not detected or was similar to expression on untreated wood (p = 0.471). The other F. radiculosa isolate (FP-90848-T) downregulated FIBRA_00974 (p = 0.301) and FIBRA_04716 (p = 0.004) on copper-treated wood. FIBRA_01430 expression by G. trabeum was not detected, but was upregulated by both F. radiculosa FP-90848-T (p = 0.481) and L-9414-SP (p = 0.392). Results from this study suggest that all three test fungi utilized different mechanisms when decaying copper-treated wood. Additionally, results from this study do not provide support for the involvement of these putative gene annotations for copper-transporting ATPase pumps in the mechanism of copper-tolerance.

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supporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Gene Expression Analysis of Three Putative Copper-Transporting ATPases in Copper-Tolerant Fibroporia radiculosa

2020

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“…It is likely that early failures in CZDP treated stakes were caused by a copper-tolerant fungus which has been frequently observed at the Mississippi test site . That copper-tolerant fungus has not been reported in the Wisconsin decking specimens, perhaps because copper-tolerant fungi are thought to be less capable of degrading copper-treated wood above-ground (Choi et al 2002, Ohno et al 2015). The laboratory leaching study indicates that much of the boron would have been depleted from the BC decking within a few years, leaving copper as the only biocide.…”

Section: Czdp Deckingmentioning

confidence: 99%

“…However, copper is not effective against all types of fungi. Some mold/ stain fungi can grow on copper-treated wood, and certain types of decay fungi are classified as "copper-tolerant" (Ohno et al 2015, Ohno 2016. These fungi can sporadically cause severe and rapid damage in wood treated with copper, and thus commercial copper-based preservatives typically include a co-biocide such as arsenic, quaternary ammonium compounds, triazoles, or naphthenic acids to provide additional protection.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of alternative copper-based preservatives in protecting decking from biodegradation

Lebow

Ohno

Lebow

et al. 2020

Maderas, Cienc. tecnol.

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The above-ground performance of decking treated with two alternative copper-based preservative formulations is being evaluated at a test site near Madison, Wisconsin, USA. Southern pine sapwood lumber specimens (38 mm by 140 mm by 910 mm) were pressure treated with 0,93 %, 1,40 % or 2,34 % (oxide basis) actives concentrations of a boron-copper formulation (BC) composed of 7,2 % copper hydroxide and 92,8 % sodium tetraborate decahydrate. Similar specimens were pressure-treated with 0,66 % or 1,32 % actives concentrations of a copper-zinc formulation (CZDP) composed of 18 % copper (CuO basis), 12 % zinc (ZnO basis), 14 % dimethylcocoamine and 56 % propanoic acid. In both cases untreated specimens and specimens treated with a 1% concentration of chromated copper arsenate Type C (CCA-C) were included for comparison. The specimens were installed on racks approximately 760 mm above the ground and periodically evaluated for extent of fungal decay and surface microbial growth. After 18 years in test specimens treated with the lowest solution concentration of BC (0,93 %) suffered substantial degradation and all but three replicates have failed. Obvious decay has not yet been detected in specimens treated to the highest BC concentration (2,34 %), but decay is suspected in one of these specimens. Decking specimens treated with CZDP exhibited no evidence of decay until year 17 when a fruiting body was observed on one specimen treated with a 0,66 % solution concentration. There has been no evidence of decay in specimens treated with 1,32 % CZDP or in either set of specimens treated with 1 % CCA-C. Both BC and CZDP-treated specimens were at least as effective as 1 % CCA-C in minimizing noticeable surface microbial growth. These decking studies confirm that relatively low copper concentrations can provide substantial protection for decking exposed in a moderate climate, and that the CZDP formulation is potentially more effective than the BC formulation. However, caution is warranted in extrapolating these findings to more severe climates and to construction designs that are more likely to trap moisture.

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“…Há estudos que sugerem que mecanismos de defesa podem ser ativados pela presença de metais, por conta da toxicidade e prejuízos que podem ocasionar à célula. Associado ao aumento das ERO pode ocorrer a produção de antioxidantes, (BELLION et al, 2006;GADD, 1999;OHNO et al, 2015). Os ácidos orgânicos podem ter o seu papel na resistência de fungos à metais, mas isso não vale para todos os fungos e depende do metal e da sua habilidade em formar complexos estáveis com ácidos aniônicos, das condições de cultivo das espécies, da fonte de nitrogênio e da concentração do metal (SAZANOVA et al, 2015).…”

Section: A Biorremediação Em Resíduos E Locais Contaminados Por Metaisunclassified

Avaliação do potencial de biorremediação por fungos filamentosos presentes em áreas de processamento de cobre.

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Insights into the mechanism of copper-tolerance in Fibroporia radiculosa: The biosynthesis of oxalate

Cited by 18 publications

References 23 publications

Gene Expression Analysis of Three Putative Copper-Transporting ATPases in Copper-Tolerant Fibroporia radiculosa

Gene Expression Analysis of Three Putative Copper-Transporting ATPases in Copper-Tolerant Fibroporia radiculosa

Efficacy of alternative copper-based preservatives in protecting decking from biodegradation

Avaliação do potencial de biorremediação por fungos filamentosos presentes em áreas de processamento de cobre.

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