Insights into the molecular basis of biocontrol of Botrytis cinerea by Clonostachys rosea in tomato

Meng, Fanyue; Lv, Rui; Cheng, Mozhen; Mo, Fulei; Zhang, Nian; Qi, Huayu; Liu, Jiayin; Chen, Xiuling; Liu, Yuxin; Ghanizadeh, Hossein; Wang, Aoxue

doi:10.1016/j.scienta.2021.110547

Cited by 25 publications

(11 citation statements)

References 48 publications

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“…WRKY transcription factors were upregulated in wheat and barley tissues in response to F. graminearum infection [ 44 , 45 ] and specifically, WRKY23 and WRKY70 were shown to participate in activating defence against F. graminearum, correlating with resistance [ 46 , 47 ]. It was demonstrated that C. rosea upregulates genes in the WRKY family and other transcription factors in tomato [ 48 , 49 , 50 ], but, to our knowledge, no such transcription factor activation by C. rosea has been demonstrated in cereals.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Effect of Clonostachys rosea on Fusarium graminearum Infection and Mycotoxin Detoxification in Oat (Avena sativa)

Khairullina

Mićić

Jørgensen

et al. 2023

Plants

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Oat (Avena sativa) is susceptible to Fusarium head blight (FHB). The quality of oat grain is threatened by the accumulation of mycotoxins, particularly the trichothecene deoxynivalenol (DON), which also acts as a virulence factor for the main pathogen Fusarium graminearum. The plant can defend itself, e.g., by DON detoxification by UGT-glycosyltransferases (UTGs) and accumulation of PR-proteins, even though these mechanisms do not deliver effective levels of resistance. We studied the ability of the fungal biocontrol agent (BCA) Clonostachys rosea to reduce FHB and mycotoxin accumulation. Greenhouse trials showed that C. rosea-inoculation of oat spikelets at anthesis 3 days prior to F. graminearum inoculation reduced both the amount of Fusarium DNA (79%) and DON level (80%) in mature oat kernels substantially. DON applied to C. rosea-treated spikelets resulted in higher conversion of DON to DON-3-Glc than in mock treated plants. Moreover, there was a significant enhancement of expression of two oat UGT-glycosyltransferase genes in C. rosea-treated oat. In addition, C. rosea treatment activated expression of genes encoding four PR-proteins and a WRKY23-like transcription factor, suggesting that C. rosea may induce resistance in oat. Thus, C. rosea IK726 has strong potential to be used as a BCA against FHB in oat as it inhibits F. graminearum infection effectively, whilst detoxifying DON mycotoxin rapidly.

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Section: Introductionmentioning

confidence: 99%

Biocontrol Effect of Clonostachys rosea on Fusarium graminearum Infection and Mycotoxin Detoxification in Oat (Avena sativa)

Khairullina

Mićić

Jørgensen

et al. 2023

Plants

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“…According to reports, B. cinerea is the second‐most common plant pathogen 5 . Chemical fungicides including diethofencarb, carbendazim, azoxystrobin, pyrimethanil, boscalid, pyraclostrobin, fluazinam, fludioxonil, procymidone, and others are the current go‐to approach for preventing and controlling B. cinerea 6 . In addition, plant essential oils, plant secondary metabolites, and biocontrol bacterium have all been shown to play an inhibitory role in the infection of B. cinerea .…”

Section: Introductionmentioning

confidence: 99%

“…5 Chemical fungicides including diethofencarb, carbendazim, azoxystrobin, pyrimethanil, boscalid, pyraclostrobin, fluazinam, fludioxonil, procymidone, and others are the current go-to approach for preventing and controlling B. cinerea. 6 In addition, plant essential oils, plant secondary metabolites, and biocontrol bacterium have all been shown to play an inhibitory role in the infection of B. cinerea. However, the long-term usage of chemical fungicides with a single chemical fungicide can cause '3R' problems (Resistance, Resurgence and Residue) in the process of biochemical control.…”

Section: Introductionmentioning

confidence: 99%

Exploring boron applications in modern agriculture: Antifungal activities and mechanisms of phenylboronic acid derivatives

Luo

et al. 2023

Pest Management Science

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Background The unreasonable use of chemical fungicides causes common adverse consequences that not only affect the environment, but also cause resistance and resurgence problems of plant pathogens, which are extremely harmful to human health, the economy, and the environment. Based on the rich biological activities of boron‐based compounds, 82 phenylboronic acid derivatives were selected and their antifungal activities against six agricultural plant pathogens were determined. Combined with transcriptomics tools, the mechanism of action of compound A49 (2‐chloro‐5‐trifluoromethoxybenzeneboronic acid) against Botrytis cinerea Pers (B. cinerea) was studied. Results The EC50 values of compounds A24, A25, A30, A31, A36, A41, A49 and B23 against all six fungi were under 10 μg/mL. Compound A49 displayed significant activity against B. cinerea (EC50 = 0.39 μg/mL), which was better than that of commercial fungicide boscalid (EC50 = 0.55 μg/mL). A49 not only inhibited the germination of B. cinerea spores, but also caused abnormal cell morphology, loss of cell membrane integrity, enhanced cell membrane permeability, and accumulation of intracellular reactive oxygen species. Further findings showed that A49 reduced cellular antioxidant activity, and peroxidase and catalase activities. Transcriptomic results indicated that A49 could degrade intracellular redox processes and alter the metabolism of some amino acids. Meanwhile, A49 showed obvious activity in vivo and low cytotoxicity to mammal cells. Conclusion The boron‐containing small molecule compounds had high efficiency and broad‐spectrum antifungal activities against six plant pathogens, and are expected to be candidate compounds for a new class of antifungal drugs. © 2023 Society of Chemical Industry.

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“…[ 6 , 7 , 8 ], Gliocladium spp. [ 9 , 10 , 11 ] and Coniothyrium minitans [ 12 , 13 ] are the most widely used biocontrol agents in the greenhouse and the field. However, unpredictable efficacy in the field sometimes hinders their application [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Monitoring Mycoparasitism of Clonostachys rosea against Botrytis cinerea Using GFP

Hasan

Uddin

et al. 2022

JoF

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Clonostachys rosea is an important mycoparasite, with great potential for controlling numerous plant fungal diseases. Understanding the mechanisms and modes of action will assist the development and application of this biocontrol fungus. In this study, the highly efficient C. rosea 67-1 strain was marked with the green fluorescent protein (GFP), and the transformant possessed the same biological characteristics as the wild-type strain. Fungal interactions with Botrytis cinerea during co-culture and encounter on tomato leaves were assessed by fluorescence confocal and electron microscopy. The results indicated that once the two fungi met, the hyphae of C. rosea grew alongside those of B. cinerea, then attached tightly to the host and developed special structures, via which the biocontrol fungus penetrated the host and absorbed nutrients, eventually disintegrating the cells of the pathogen. Mycoparasitism to B. cinerea was also observed on tomato leaves, suggesting that C. rosea can colonize on plants and act following the invasion of the pathogenic fungus.

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Insights into the molecular basis of biocontrol of Botrytis cinerea by Clonostachys rosea in tomato

Cited by 25 publications

References 48 publications

Biocontrol Effect of Clonostachys rosea on Fusarium graminearum Infection and Mycotoxin Detoxification in Oat (Avena sativa)

Biocontrol Effect of Clonostachys rosea on Fusarium graminearum Infection and Mycotoxin Detoxification in Oat (Avena sativa)

Exploring boron applications in modern agriculture: Antifungal activities and mechanisms of phenylboronic acid derivatives

Monitoring Mycoparasitism of Clonostachys rosea against Botrytis cinerea Using GFP

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