Insights into the phenomenon of acquisition and accumulation of Fe3+ in Hygrophila spinosa through fluorimetry and fluorescence images

Ghosh, Ayndrila; Mandal, Saurodeep; Sahoo, Prithidipa; Shaw, Pallab; Chattopadhyay, Ansuman

doi:10.1016/j.tetlet.2019.151520

Cited by 10 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies with L-02 cells and PC-12 rat pheochromocytoma cells confirmed its application in detecting exogenous Fe(III) in subcellular regions. Dyes 78 , 79 , 80a , and 81 – 83 similarly contain an amide linkage between the rhodamine and recognition unit, and respond to Fe(III) through a spirolactam ring-opening. − In addition, dyes 80b , 84 , and 85 are examples in which two rhodamine units were joined together in such a manner that the probe can coordinate two ferric ions simultaneously, resulting into a strong turn-on response (560-585 nm) owing to the ring opening of rhodamine. ,, These probes were then employed for cellular imaging of Fe(III) ions in PC-12, human gastric cell line (SGC-7901) and MCF-7 cells respectively. Dyes RBCS ( 86 ) and RBTM ( 87 ) are two thioamide-containing rhodamine B-based probes that selectively bind to Fe(III) in the ring-opened form to produce a strong fluorescence emission. , These probes were employed in HeLa and MKN-45 human gastric cancer cells, respectively, to visualize exogenously added ferric ions.…”

Section: Fluorescent Sensors For Ironmentioning

confidence: 99%

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Grover,

Koblova,

Pezacki

et al. 2024

Chem. Rev.

View full text Add to dashboard Cite

Although transition metals constitute less than 0.1% of the total mass within a human body, they have a substantial impact on fundamental biological processes across all kingdoms of life. Indeed, these nutrients play crucial roles in the physiological functions of enzymes, with the redox properties of many of these metals being essential to their activity. At the same time, imbalances in transition metal pools can be detrimental to health. Modern analytical techniques are helping to illuminate the workings of metal homeostasis at a molecular and atomic level, their spatial localization in real time, and the implications of metal dysregulation in disease pathogenesis. Fluorescence microscopy has proven to be one of the most promising non-invasive methods for studying metal pools in biological samples. The accuracy and sensitivity of bioimaging experiments are predominantly determined by the fluorescent metal-responsive sensor, highlighting the importance of rational probe design for such measurements. This review covers activity-and binding-based fluorescent metal sensors that have been applied to cellular studies. We focus on the essential redox-active metals: iron, copper, manganese, cobalt, chromium, and nickel. We aim to encourage further targeted efforts in developing innovative approaches to understanding the biological chemistry of redox-active metals. CONTENTS5908 8.2. Activity-Based Fluorescent Sensors for Ni(II) 5911 9. Outlook 5911 Author Information 5912 Corresponding Authors 5912

show abstract

Section: Fluorescent Sensors For Ironmentioning

confidence: 99%

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Grover,

Koblova,

Pezacki

et al. 2024

Chem. Rev.

View full text Add to dashboard Cite

show abstract

“…Interestingly, a number of "turn-on" or ratio metric probes for Fe 3þ have recently been reported (Aydin, Wei, and Guo 2012;Wei et al 2012;Zhu et al 2016;Zhou et al 2017;Qiao et al 2018;Wu et al 2019;Shellaiah et al 2020;Ghosh et al 2020;Li et al 2016;Liu et al 2020;Yang et al 2020;Kaur, Kaur, and Kumar 2018;Zhang et al 2014;Lohani and Lee 2010;Heng et al 2008;Ackerman, Lee, and Chang 2017;Lee et al 2016;Wang et al 2010;Mir et al 2019;Qiu et al 2014;Vishaka et al 2019;Kim et al 2019;Jothi et al 2021;Gao et al 2020;Zhang et al 2021;Wei et al 2010). Many iron probes, especially turn-on and ratiometric Fe 3þ probes, have been discussed in detail in recent review papers Crisponi 2019, Sun, Sun, andGuo 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Table S1 (supplementary material) summarizes the basic properties and performance parameters of the recent Fe 3þ sensors. However, their capabilities in unambiguously detecting native cellular Fe 3þ pools across the entire biological pH range have yet to be established due to their various intrinsic drawbacks (Domaille, Que, and Chang 2008;Zhu et al 2016;Zhou et al 2017;Qiao et al 2018: Wu et al 2019Shellaiah et al 2020;Ghosh et al 2020;Liu et al 2020;Yang et al 2020). Reaction-based Fe 3þ -sensors displayed nice turn-on fluorescent responses with cellular imaging capability but are incapable of monitoring the dynamic changes in Fe 3þ due to the irreversible nature of the catalytic hydrolysis reactions (Kaur, Kaur, and Kumar 2018;Zhang et al 2014;Lohani and Lee 2010;Heng et al 2008;Ackerman, Lee, and Chang 2017;Lee et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Highly Selective Fluorescent Probe With an Ideal pH Profile for the Rapid and Unambiguous Determination of Subcellular Labile Iron (III) Pools in Human Cells

et al. 2022

View full text Add to dashboard Cite

A convenient tool for detecting iron ions in subcellular structures is desired for better understanding its roles in biological systems. In this work, a new Fe 3þ sensor, 2-(2-((1-(6-acetylpyridin-2-yl)ethylidene)amino)ethyl)-3',6'-bis(diethylamino)spiro[isoindoline-1,9'-xanthen]-3-one (RhPK), which operates across the entire cellular pH range and is capable of unambiguously detecting Fe 3þ ion in live human cells at subcellular resolution, is reported. The sensor exhibits high selectivity and sensitivity toward Fe 3þ with a rapid fluorescence response and a 12-fold increase in intensity upon the addition of 1 equivalent Fe 3þ at pH 7.3. RhPK forms a 1:1 complex with Fe 3þ with an apparent binding constant 1.54 Â 10 7 M À1 and a detection limit of 50 nM. The sensor is stable between pH 4.2 and 9.0 and operates across the whole cellular pH range. Cell imaging demonstrates the ability of the sensor to unambiguously detect basal level Fe 3þ as well as its dynamic changes in real-time in live cells at subcellular resolution, with one labile Fe 3þ pool identified in mitochondria in human primary fibroblast (ws1) cells for the first time and two Fe 3þ pools confirmed in mitochondria and endo/lysosomes in human SH-SY5Y neuroblastoma cells, suggesting different cell types have distinctive Fe 3þ storage in subcellular compartments. The RhPK probe is powerful for rapid and sensitive bioimaging of Fe 3þ at subcellular level, enabling the unambiguous detection of labile Fe 3þ pools at the entire cellular pH range, which is of great significance to understand the biological chemistry of Fe 3þ and its roles in physiological processes and diseases.

show abstract

“…However, their disadvantages including low specificity, complicated sample preparation, and expensive instruments hindered their wide applications. − Recently, increasingly more attention has been paid to the fluorescence method for the detection of Fe 3+ because of its ability to detect Fe 3+ rapidly, sensitively, and selectively. And it could not cause any damage to cell. − To date, many fluorescent probes for Fe 3+ have been synthesized successfully, which included coumarin, , anthracene, BODIPY, , cyanine, and rhodamines . Among these probes, rhodamine 6G has many advantages over other derivatives, including high extinction coefficients, high quantum yields, excellent photostability, and emission wavelengths. − However, most rhodamine derivatives were obtained through CN, but a few compounds linked by amide have been reported. − It was clear that the amide-modified rhodamine 6G derivatives have more potential coordination sites to bind metal ions than CN .…”

Section: Introductionmentioning

confidence: 99%

Novel Fluorescent Probe toward Fe³⁺ Based on Rhodamine 6G Derivatives and Its Bioimaging in Adult Mice, Caenorhabditis elegans, and Plant Tissues

Zhang

Chen

et al. 2021

ACS Omega

View full text Add to dashboard Cite

A new fluorescent probe LXY based on the rhodamine 6G platforms has been designed, synthesized, and characterized, which could recognize Fe3+ effectively in HEPES buffer (10 mM, pH = 7.4)/CH3CN (2:3, v/v). And the distinct color change and the rapid emergence of fluorescence emission at 550 nm achieved “naked eye” detection of Fe3+. The interaction mode between them was achieved by Job’s plot, MS, SEM, and X-ray single-crystal diffraction. Importantly, the crystal structures proved that Fe3+ could induce the rhodamine moiety transform the closed-cycle form to the open-cycle form. But it is interesting that Fe3+ did not appear in the crystal structures. Meanwhile, the limit of detection (LOD) of LXY to Fe3+ was calculated to be 3.47 × 10–9. In addition, the RGB experiment, test papers, and silica gel plates all indicated that the probe LXY could be used to distinguish Fe3+ quantitatively and qualitatively on-site. Moreover, the probe LXY has also been successfully applied to Fe3+ image in Caenorhabditis elegans, adult mice, and plant tissues. Thus, LXY was considered to have some potential for application in bioimaging.

show abstract

Insights into the phenomenon of acquisition and accumulation of Fe3+ in Hygrophila spinosa through fluorimetry and fluorescence images

Cited by 10 publications

References 28 publications

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Highly Selective Fluorescent Probe With an Ideal pH Profile for the Rapid and Unambiguous Determination of Subcellular Labile Iron (III) Pools in Human Cells

Novel Fluorescent Probe toward Fe³⁺ Based on Rhodamine 6G Derivatives and Its Bioimaging in Adult Mice, Caenorhabditis elegans, and Plant Tissues

Contact Info

Product

Resources

About

Insights into the phenomenon of acquisition and accumulation of Fe3+ in Hygrophila spinosa through fluorimetry and fluorescence images

Cited by 10 publications

References 28 publications

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Highly Selective Fluorescent Probe With an Ideal pH Profile for the Rapid and Unambiguous Determination of Subcellular Labile Iron (III) Pools in Human Cells

Novel Fluorescent Probe toward Fe3+ Based on Rhodamine 6G Derivatives and Its Bioimaging in Adult Mice, Caenorhabditis elegans, and Plant Tissues

Contact Info

Product

Resources

About

Novel Fluorescent Probe toward Fe³⁺ Based on Rhodamine 6G Derivatives and Its Bioimaging in Adult Mice, Caenorhabditis elegans, and Plant Tissues