Insights into the Supercritical CO2 Extraction of Perilla Oil and Its Theoretical Solubility

Abstract

In the current research, the supercritical carbon dioxide (SCCO2) procedure was used to extract volatile oils from perilla leaves. The yields of the volatile oils and the four main constituents, limonene, perillaldehyde, β-caryophyllene, and (Z,E)-α-farnesene obtained by the SCCO2 procedure were 1.31-, 1.12-, 1.04-, 1.05-, and 1.07-fold higher than those obtained by the hydrodistillation technique, respectively. Furthermore, the duration and temperature of extraction were 40 min and 45 °C lower, respectively, in the former procedure compared to the latter technique. These advantages reveal that SCCO2 not only obtains high-quality extracts, but also meets the requirements of green environmental protection. The theoretical solubilities of the volatile oils acquired by the SCCO2 dynamic extraction at various temperatures and pressures were 1.385 × 10−3–8.971 × 10−3 (g oil/g CO2). Moreover, the three density-based models were well correlated with these theoretical solubility data, with a high coefficient of determination and low average absolute relative deviation.

Chemical Characterization of Chinese Perilla Seed Oil

“…Perilla seed oil is now used to make health care drugs, such as capsules and oil drops, in China. Several extraction methods of the perilla oil were investigated, such as solvent extraction, aqueous enzymatic extraction, mechanical pressing and supercritical extraction 8) . Supercritical extraction and aqueous enzymatic extraction methods are costly, and solvent extraction is effective and commonly used.…”

Process optimization in the extract of perilla seed oil with plant protein hydrolysate complex enzyme

“…The existing techniques used for oil extraction from seed of different origins include soxhlet extraction, organic solvent extraction, cold-pressing, aqueous enzymatic extraction and supercritical carbon dioxide (Jia et al, 2013;Ahmed et al, 2019;Jung et al, 2012;Yusoff et al, 2015;Hao et al, 2021;Wei et al, 2021). Soxhlet extraction takes a long time and consumes a lot of solvents (Mohammadpour et al, 2019;Castro & Priego-Capote, 2010).…”