Insights into the Thermophile Diversity in Hot Springs of Pakistan

Amin, Arshia; Ahmed, Iftikhar; Khalid, Nauman; Zhang, Yao; Xiao, Min; Li, Wenjun

doi:10.1007/978-981-13-0329-6_1

Cited by 5 publications

(2 citation statements)

References 125 publications

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“…The application of eDNA has proved to be rapid and efficient for surveying biodiversity in ecosystems (Thomsen & Willerslev, 2015). Although studies have focused on using eDNA in springs (Amin et al., 2018; D’Auria et al., 2018; Vörös et al., 2017), including desert springs (Paulson & Martin, 2019), few freshwater studies have focused on the detection of organisms across diverse taxonomic groups (Belle et al., 2019). Similarly, eDNA sediment and water samples have been compared in only a few studies but have consistently found sedimentary eDNA to have a lower decay rate and higher concentration of eDNA (Buxton et al., 2018; Turner et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

The utility of environmental DNA from sediment and water samples for recovery of observed plant and animal species from four Mojave Desert springs

et al. 2020

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Mojave Desert springs are fragile ecosystems, hosting endemic plants and animals, which are threatened by the increasing human demand for water and climate change. To develop management practices that will protect the groundwater‐dependent ecosystems at Mojave Desert springs, real‐time, low‐cost biodiversity monitoring, and assessments are required. Environmental DNA (eDNA) metabarcoding uses DNA shed from organisms (e.g., skin cells, feces, and pollen) that are present in water, air, soil, or sediment samples to assess community composition. This approach can increase the detection sensitivity for rare and elusive species, compared with expensive and time‐consuming conventional methods, which also require taxonomic expertise. This study tests the effectiveness of eDNA techniques in capturing the observed Mojave Desert spring biodiversity in the winter and spring of 2019 at four distinct, naturally occurring springs. We also test the utility of sample types (water vs. sediment) for capturing biodiversity. We found that each of the four Mojave Desert springs supports a unique biological community. Sediment samples contained the greatest biodiversity, but all sample types captured species observed in the field by humans or camera traps. We also found no statistical difference in species richness captured in winter and spring except for the Cytochrome Oxidase I marker, for which winter had greater biodiversity. This study supports the use of eDNA metabarcoding as an effective tool to mirror observation by human observers of ecological communities in desert springs. The study demonstrates the importance of appropriately timing eDNA field sampling, primer selection, and using field‐based surveys of wildlife and plants in addition to eDNA detection. This study also identified gaps in reference sequence databases for Mojave biodiversity and encourages collaboration of eDNA researchers with managers for effective conservation management plans.

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Section: Introductionmentioning

confidence: 99%

The utility of environmental DNA from sediment and water samples for recovery of observed plant and animal species from four Mojave Desert springs

et al. 2020

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“…Thermophiles have optimum growth temperature of >55 • C and in general, many of them are polyextremophiles, which means that they are capable of living in other extreme environmental conditions, such as those related to pH, redox potential, salt concentration or the presence of a wide spectrum of toxic compounds [8][9][10]. Thermophiles are mainly found in hot springs [11][12][13], hot springs adjacent to volcanic environments [14][15][16], upper soil layers [17]-and even in man-made thermal environments, such as compost facilities [18]. Therefore, different research groups have reported important works related with the characterization of microorganisms in aqueous media [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Thermophilic Microorganisms in the Geothermal Water Flow of El Chichón Volcano Crater Lake

Ovando-Chacón

Tacias-Pascacio

Ovando-Chacon

et al. 2020

Water

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This study reports for the first time the isolation, identification and characterization of lipase-producing thermophilic strain from the geothermal water of the El Chichón volcano crater lake. Two strains were identified by 16S rRNA sequencing as Geobacillus jurassicus CHI2 and Geobacillus stearothermophilus CHI1. Results showed that G. jurassicus CHI2 is Gram-positive, able to ferment maltose, fructose and sucrose and to hydrolyze starch and casein; while G. stearothermophilus CHI1 showed to be Gram-variable, able to ferment maltose and fructose and to hydrolyze starch. Colonies of both strains presented irregular shape, umbilicated elevation of gummy texture and cells presented flagellar movement to survive in fluids with high temperature and mass gradients due to complex phenomena of heat and mass transfer present in the geothermal fluids. Lipase production for G. stearothermophilus CHI1 was also evaluated. It was found that this strain possesses a growth associated with extracellular lipase production with a high activity of 143 U/mL at 8.3 h of incubation time, superior to the activities reported for other microorganisms of genus Geobacillus; for this reason, it can be said that the thermal flow of the El Chichón volcano crater lake can be a useful source of lipase-producing thermophilic bacteria.

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The Geochemistry of Hot Springs

Erfurt¹

2021

The Geoheritage of Hot Springs

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Insights into the Thermophile Diversity in Hot Springs of Pakistan

Cited by 5 publications

References 125 publications

The utility of environmental DNA from sediment and water samples for recovery of observed plant and animal species from four Mojave Desert springs

The utility of environmental DNA from sediment and water samples for recovery of observed plant and animal species from four Mojave Desert springs

Characterization of Thermophilic Microorganisms in the Geothermal Water Flow of El Chichón Volcano Crater Lake

The Geochemistry of Hot Springs

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