Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Hettle, A.G.; Hobbs, Joanne K.; Pluvinage, B.; Vickers, Chelsea; Abe, Kento T.; Salama-Alber, Orly; McGuire, Bailey E.; Hehemann, Jan‐Hendrik; Hui, Joseph P. M.; Berrué, Fabrice; Banskota, Arjun H.; Zhang, Junzeng; Bottos, Eric; Hamme, Jonathan D. Van; Boraston, A.B.

doi:10.1038/s42003-019-0721-y

Cited by 54 publications

(64 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SY01 is recorded in addition to the three from this study. Nevertheless, a recent study identified a κ/ι-carrageenan metabolism pathway in several marine Pseudoalteromonas species, with a κ/ι-carrageenan-specific polysaccharide utilization locus (CarPUL) responsible for this phenotype ( Hettle et al, 2019 ). Within the CarPUL of Pseudoalteromonas fuliginea , three GH16_17-coding genes namely GH16A (EU509_08815), GH16B (EU509_08870), and GH16C (EU509_08960) were separated with several intervening genes including sulfatase and major facilitator superfamily (MFS) transporter genes.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the GH16_17s from HN897 shared 40–57% sequence identity with those of P. fuliginea. Given that both HN897 and C7 have orthologous genes associated with carrageenan metabolism (see Supplementary Table S5 ; Hettle et al, 2019 ), it was tempting to speculate that V. astriarenae has the ability to metabolize galactan carrageenan.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Identification and Functional Characterization of β-Agarases in Vibrio astriarenae Strain HN897

Liu

Jin

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

The genus Vibrio is a genetically and metabolically versatile group of heterotrophic bacteria that are important contributors to carbon cycling within marine and estuarine ecosystems. HN897, a Vibrio strain isolated from the coastal seawater of South China, was shown to be agarolytic and capable of catabolizing D -galactose. Herein, we used Illumina and PacBio sequencing to assemble the whole genome sequence for the strain HN897, which was comprised of two circular chromosomes (Vas1 and Vas2). Genome-wide phylogenetic analysis with 140 other Vibrio sequences firmly placed the strain HN897 into the Marisflavi clade, with Vibrio astriarenae strain C7 being the closest relative. Of all types of carbohydrate-active enzyme classes, glycoside hydrolases (GH) were the most common in the HN897 genome. These included eight GHs identified as putative β-agarases belonging to GH16 and GH50 families in equal proportions. Synteny analysis showed that GH16 and GH50 genes were tandemly arrayed on two different chromosomes consistent with gene duplication. Gene knockout and complementation studies and phenotypic assays confirmed that Vas1_1339 , a GH16_16 subfamily gene, exhibits an agarolytic phenotype of the strain. Collectively, these findings explained the agar-decomposing of strain HN897, but also provided valuable resources to gain more detailed insights into the evolution and physiological capability of the strain HN897, which was a presumptive member of the species V. astriarenae .

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Functional Characterization of β-Agarases in Vibrio astriarenae Strain HN897

Liu

Jin

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Unlike animals, sulfatases have not yet conclusively been identified or characterized in red algae. It is unknown how red algae cope with ECM remodeling in the absence of known sulfatases, though the red algal microbiome has a role in the recycling of sulfur as it produces sulfatases specific for carrageenan [71][72][73][74] .…”

Section: Gene Annotationmentioning

confidence: 99%

To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus

Lipinska

Collén

Krueger‐Hadfield

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix. Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n). The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iotacarrageenans). The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles. We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France. Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydratesulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase. Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes. The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae. Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus. Abbreviations ECM Extracellular matrix GAG Glycosaminoglycan GH Glycoside hydrolase GT Glycosyl transferase ST Sulfotransferase Red algae are ancient eukaryotic organisms, providing the first clearly identified fossil record of eukaryotic photosynthetic life and the earliest known extant form of complex multicellularity 1-3. A prerequisite for complex multicellularity is the development of an extracellular matrix (ECM). While cellulose is present in red algae, the

show abstract

“…P. fuliginea sp. PS47 is a recently identified marine bacterium that possesses the ability to process algal galactans (Hettle et al, 2019), including a potential genetic capacity for agarose degradation and metabolism.…”

Section: Introductionmentioning

confidence: 99%

The structure of PfGH50B, an agarase from the marine bacterium Pseudoalteromonas fuliginea PS47

et al. 2020

Self Cite

View full text Add to dashboard Cite

The recently identified marine bacterium Pseudoalteromonas fuliginea sp. PS47 possesses a polysaccharide-utilization locus dedicated to agarose degradation. In particular, it contains a gene (locus tag EU509_06755) encoding a β-agarase that belongs to glycoside hydrolase family 50 (GH50), PfGH50B. The 2.0 Å resolution X-ray crystal structure of PfGH50B reveals a rare complex multidomain fold that was found in two of the three previously determined GH50 structures. The structure comprises an N-terminal domain with a carbohydrate-binding module (CBM)-like fold fused to a C-terminal domain by a rigid linker. The CBM-like domain appears to function by extending the catalytic groove of the enzyme. Furthermore, the PfGH50B structure highlights key structural features in the mobile loops that may function to restrict the degree of polymerization of the neoagaro-oligosaccharide products and the enzyme processivity.

show abstract

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Cited by 54 publications

References 66 publications

Genome-Wide Identification and Functional Characterization of β-Agarases in Vibrio astriarenae Strain HN897

Genome-Wide Identification and Functional Characterization of β-Agarases in Vibrio astriarenae Strain HN897

To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus

The structure of PfGH50B, an agarase from the marine bacterium Pseudoalteromonas fuliginea PS47

Contact Info

Product

Resources

About