Insights into total estrogenic activity in a sewage-impacted urban stream assessed via ER transcriptional activation assay: Distribution between particulate and dissolved phases

Argolo, Allan dos Santos; Gomes, Giselle; Bila, Daniele Maia

doi:10.1016/j.ecoenv.2020.111574

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…38,39,40 In another study, the relevance of quantifying the total estrogenic activity of aqueous environmental matrices considering the solid fractions was also shown. 18 The vacuum centrifugation and lyophilization methods used to concentrate substances with estrogenic activity are methods that do not require the use of chemical solvents for the concentration of samples. In this sense, they are less polluting compared to the SPE method, in which solvents such as methanol, hexane, are used.…”

Section: Resultsmentioning

confidence: 99%

“…Calculation of the corrected absorbance of the data from each well of the assay microplate was performed, using the absorbance values obtained at the two wavelengths for each concentration of the curve, according to the calculation presented in Argolo et al 18 As a control of the YES assay, the growth inhibition of the yeast Saccharomyces cerevisiae was quantified due to the toxicity of the samples, according to Frische et al 32 Sigmoidal curves were obtained using a symmetric logistic function using the Origin 6.0 program (Microsoft, USA). The estrogenic activity in each well containing sample extract was then calculated as estradiol equivalents (EQ-E2) in ng L -1 , by interpolation with the E2 dose-response curve.…”

Section: Yes (Yeast Estrogen Screen) In Vitro Assaymentioning

confidence: 99%

“…Before analyzing the estrogenic activity in water, it is necessary to concentrate the substances present in the samples. The method commonly used for concentration is the one that uses a cartridge (with adsorbent matrix), i.e., the solid-phase extraction (SPE) method 18,19 This technique is efficient, but it is expensive, and uses solvents such as hexane and methanol, that pollute the environment and pose a risk to human health. 20,21 Although less used than SPE to concentrate these substances, freeze-drying and vacuum centrifugation methods have also been used.…”

Section: Introductionmentioning

confidence: 99%

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Estrogenic activity in reused water: comparison of concentration methods

Handam¹

2021

IJH

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Determining the presence of endocrine disrupting substances in waters is a relevant aspect for monitoring environmental health. Given its relevance, it is important to use methods that can make the total concentration of substances with estrogenic activity (eg endocrine disruptors), being faster, and without the use of compounds that pollute the environment. The purpose of the study was to compare the effectiveness of the methods of concentration by lyophilization and by vacuum concentration of substances with estrogenic activity present in reused water, using the commonly used methodology, solid phase extraction. Three methods were compared: solid phase extraction, lyophilization, and vacuum centrifugation. Sample aliquots of reused water received 17β-estradiol at a final concentration of 2 μg L-1 and were concentrated by the three methods. The analysis of estrogenic activity was performed by the in vitro YES (Yeast Estrogen Screen) assay. The results showed that the vacuum centrifugation, solid phase extraction and lyophilization methods had different percentages in the recovery of substances with estrogenic activity, being 45%, 40%, and 31%, respectively. The study pointed out that the lyophilization and vacuum centrifugation methods were effective as alternative methods for concentrating samples containing substances with estrogenic activity.

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Section: Resultsmentioning

confidence: 99%

Section: Yes (Yeast Estrogen Screen) In Vitro Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Estrogenic activity in reused water: comparison of concentration methods

Handam¹

2021

IJH

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“…where A 1 and A 2 refer to the maximum and minimum β-galactosidase induction in corrected absorbance, x 0 is the median effect concentration EC50% for E2 in ng L −1 , p corresponds to the slope of the sigmoidal curve, and (x, y) is the ordered pair related to a sample concentration and its response in corrected absorbance. Finally, the E2-EQ was calculated as the lowest x that elucidated an agonist response divided by the final sample enrichment factor in the assay [38].…”

Section: Discussionmentioning

confidence: 99%

“…However, cytotoxicity (yeast growth inhibition) and the presence of antagonistic substances are other pieces of evidence obtained from the assay. Estrogenic activity can still be determined [36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Experimental Design and Bioassays as Tools to Investigate the Impact of Anodic Oxidation on Progestins Degradation

Jesus

Argolo

Tominaga

et al. 2022

Water

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The present study investigated the degradation of levonorgestrel (LNG) and gestodene (GES) through an anodic oxidation process mediated by active chlorine species. The independent variables [LNG]0 and [GES]0, current density (mA cm−2), and [NaCl]0 (mol L−1) were optimized through a response surface methodology (RSM) based on a four-level central composite design (CCD). Specific energy consumption allowed CCD-RSM analysis and optimization. The decay of progestins was followed to verify the kinetics of the anodic degradation process. Chlorine monitoring showed that excess Cl− concentration did not mean high hormones removal, as well as the excess of current density. Central point conditions ([NaCl]0 = 0.07 mol L−1, j = 32.5 mA cm−2, [LNG]0, and [GES]0 1.0 mg L−1) proved to be the best operational option. The performance with real pharmaceutical wastewater confirmed model optimization (2.2 ± 0.2 kWh g−1, with removals of 83.1 ± 0.9% and 75.1 ± 2.8% for LNG and GES, respectively). The selected condition was used for estrogenic activity and acute toxicity assays. The first allowed the identification of the initial estrogenic activity for the mixture of LNG and GES (924 E2-EQ ng L−1). Additionally, the electrochemical process could decrease this environmental parameter by 74.6%. The progestin mixture was classified as acute toxicity to Daphnia similis, with a toxicity unit (TU) of 2.5 100/EC50%. After electrolysis, the hormone solutions reached a fourfold increase in TU value, classified as high acute toxicity.

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