Insights of the Antimicrobial Activity of Piperine Extracted From Piper Nigrum L.

Abstract

The aim of this study was to present new insights of the antimicrobial activity of piperine extracted from Piper nigrum as compared to commercial piperine and to the activity of other similar compounds (namely: protocatechuic acid, L-ascorbic acid, L-tyrosine and syringic acid) and in synergism with ampicillin and amphotericin B. For this, piperine was isolated, purified and its structure and high purity were determined by NMR, FTIR, UV-Vis and TLC analysis. A concentration of 100 mg/mL of piperine proved to have no cytotoxic effect on the opportunistic pathogens represented by Staphylococcus aureus and Escherichia coli, and was slightly efficient against Candida albicans. Nevertheless, piperine improved the ampicillin activity against S. aureus with 14% and in the same time decreased the activity of amphotericin B against C. albicans with 54.2%, their antimicrobial properties being quite different compared to the commercial piperine. RezumatScopul acestui studiu este de a prezenta noi perspective asupra activității antimicrobiene a piperinei extrase din Piper nigrum în comparație cu piperina comercială și cu activitatea altor compuși similari (și anume: acid protocatechic, acid L-ascorbic, L-tirozină și acid siringic) și în sinergism cu ampicilina și amfotericina B. Pentru aceasta, piperina a fost izolată, purifica tă și caracterizată prin analize RMN, FTIR, UV-Vis și TLC. O concentrație de piperină de 100 mg/mL nu a prezentat efect citotoxic asupra agenților conditionat patogeni reprezentați de Staphylococcus aureus și Escherichia coli și a fost eficient împotriva speciei Candida albicans. Cu toate acestea, piperina a îmbunătățit activitatea ampicilinei împotriva speciei S. aureus cu 14% și, în același timp, a scăzut activitatea amfotericinei B împotriva levurii C. albicans cu 54,2%, proprietățile ei antimicrobiene fiind destul de diferite față de piperina comercială.

Isolation of piperine from black pepper (Piper nigrum) in the provision of standard compounds for natural chemical practice and research activities

“…st.), 1440 cm-1 (methylenedioxy CH2bending), 1581 cm-1 (arom. C=C st.), 1631 cm-1 (-CO-N-) [19]. The impurity compounds were detected at a wavenumber of 3500 cm-1.…”

Emerging Applications of Some Important Natural Compounds in the Field of Oncology

“…Kaempferol and quercetin isolated from Ginkgo biloba can trigger apoptosis in oral cancer cells through a caspase-3 dependent mechanism, successfully inhibiting cell proliferation in a dose-dependent manner [28]. Piperine is a caspase-mediated pro-apoptotic alkaloid that, in high concentrations, can suppress oral squamous cell tumour growth through ROS production increase and mitochondrial depolarization, while also causing cell cycle arrest and nuclear condensation [37,38,58].…”

Exploring the potential of spice‐derived phytochemicals as alternative antimicrobial agents

“…A study by Mgbeahuruike et al (2019) showed highly potent activities against S. aureus , P. aeruginosa and C. albicans with MIC values of 3.9–15.6 µg/mL and a zone of inhibition diameter of 14.4–16.5 mm. In contrast, Moraru et al (2019) reported piperine extract of 100 mg/mL had no inhibition on S. aureus , E. coli and weak effect on C. albicans whereby the inhibitory zone is close to zero. The mechanism of piperine inhibitory action is still poorly understood.…”