Insilco identification and characterization of superoxide dismutase gene family in Brassica rapa

Abstract

Superoxide Dismutase SODs are defense associated proteins that detoxify ROS and primarily serve as scavengers. They have been described in numerous plant species, but their in-depth characterization in Brassica rapa has not been reported. Therefore, the present investigation on genome wide study of SOD gene family was conducted to identify Br SOD genes, their domain-based organization, gene structure analysis, phylogenetic analysis, intron-exon structure of genes and expression analysis. The sequence characterization of Super oxide dismutase gene family in Brassica rapa , their syntenic associateship of conserved motifs and phylogenetic correlationship, prediction of cis -elements and determing the expression analysis in distinct tissues namely plant callus, root, stem, leaf, flower, and silique under abiotic conditions have been analysed using different software’s. The study on SOD gene family identified 17 Br SOD genes which were grouped into eight Br Cu-ZnSODs and nine Br Fe-MnSODs domain-based organization. Furthermore, the conserved character of Br SODs were confirmed by intron-exon organisation, motif arrangements and domain architectural investigations. Expression analysis using RNA Sequence data of different developmental stages proclaimed that genes were manifested in all six tissues with an exception of Br Cu-ZnSOD3, which was not manifested in roots; however, whose transcript was detected in all other tested tissues. The study has genome wide insight into the occurrence and functional specifications of Br SOD gene family in Brassica rapa that can be potentially utilized in breeding program for resilience to climate change and abiotic stresses tolerance Brassica variety.

Trends and Opportunities in Enzyme Biosensors Coupled to Metal-Organic Frameworks (MOFs): An Advanced Bibliometric Analysis

“…For example, the term "dismutase" was predominantly yellow, indicating its strong presence in recent studies. This term refers to superoxide dismutases, which are antioxidant enzymes that protect organisms from reactive oxygen species [151]. Con versely, the term "coordination polymer" appeared mostly in purple, indicating its older fixation.…”

Genome-wide identification of the rubber tree superoxide dismutase (SOD) gene family and analysis of its expression under abiotic stress

“…Taken together, these studies show that SOD genes play vital roles in the plant responses to abiotic stresses. Recently, with the rapid development of genome sequencing, the SOD gene family members of different species were comprehensively identified at the genome-wide level in many plant species, e.g., Brassica napus ( Su et al, 2021 ), A. thaliana ( Kliebenstein, Monde & Last, 1998 ), Brassica rapa ( Iqbal Qureshi et al, 2021 ), Salvia miltiorrhiza ( Han et al, 2020 ), Dendrobium catenatum ( Huang et al, 2020 ), Camellia sinensis ( Zhou et al, 2019 ), Zostera marina ( Zang et al, 2020 ), Vitis vinifera ( Hu et al, 2019 ), Gossypium hirsutum ( Wang et al, 2017 ) and Solanum lycopersicum ( Feng et al, 2016 ).…”

Mnsod1 promotes the development of Pleurotus ostreatus and enhances the tolerance of mycelia to heat stress

“…Then, the number of SOD isozymes coding genes varies with species [ 5 , 6 ]. For example, there are 8 SOD coding genes in the Arabidopsis genome, while 17 SOD isozyme coding genes have been identified in Brassica rapa [ 7 ]. In fungi, SOD has also been cloned and studied in recent years.…”