Instability of chlorophyll in yellow lupin seedlings grown in soil contaminated with ciprofloxacin and tetracycline

Rydzyński, Dariusz; Piotrowicz-Cieślak, Agnieszka I.; Grajek, Hanna; Michalczyk, Dariusz J.

doi:10.1016/j.chemosphere.2017.05.147

Cited by 45 publications

(19 citation statements)

References 39 publications

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“…Also, the intensity of fluorescence spectra was reduced and this effect was stronger in Cd than TC treatments. A similar shift of fluorescence spectra (5 nm) was observed with Chl from leaves of lupin growing in TC contaminated soil TC (Rydzyński et al 2017). The fluorescence spectra shifts confirm the formation of chlorophyll degradation product under the action of TC and Cd on plants.…”

Section: Absorption and Fluorescence Characteristics Of Chlorophyll Dsupporting

confidence: 74%

“…SPE cartridges were rinsed with methanol and after loading with plant saps were eluted with 250 µl methanol. Tetracycline content in seedlings was analyzed by HPLC (Water Alliance 2695) according to Pailler et al (2009) with small modifications by Rydzyński et al (2017).…”

Section: Tetracycline Contentmentioning

confidence: 99%

“…It has been repeatedly demonstrated that both antibiotics (Margas et al 2016;Zhao et al 2002;Rydzyński et al 2017) and cadmium (Küpper et al 2006) inhibit photosynthesis and contribute to the generation of reactive oxygen species (ROS) (Andresen and Küpper 2013). ROS cannot be formed directly in the Fenton or Haber-Weiss reaction (Wardman and Candeias 1996), but they may arise indirectly, due to the impairment of photosynthesis or respiration.…”

Section: Introductionmentioning

confidence: 99%

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Chlorophyll degradation by tetracycline and cadmium in spinach (Spinacia oleracea L.) leaves

Rydzyński

Piotrowicz-Cieślak

Grajek

et al. 2018

Int. J. Environ. Sci. Technol.

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Degradation of chlorophyll was studied in leaves of spinach grown in soil containing cadmium and tetracycline, based on spectroscopic measurements and biochemical analyses of plant extracts. It was shown that plant exposure to the highest levels of tetracycline and cadmium resulted in 64% and 68%, respectively, reduction in chlorophyll content. The chlorophyll degradation rate constants were determined, and they were found to increase with increasing doses of tetracycline and cadmium. The rate constant of chlorophyll degradation by tetracycline ranged from k = 960 M −1 day −1 to k = 2180 M −1 day −1 , and the rate constant of chlorophyll degradation by cadmium ranged from k = 1130 M −1 day −1 to k = 2580 M −1 day −1 , depending on dose. Plant stress responses to tetracycline are much stronger than to cadmium, as is visible from the activity of guaiacol peroxidase and catalase. However, phytotoxicity of cadmium, measured by the rate of chlorophyll degradation and enzyme activities, is much higher compared to tetracycline. The spectroscopic measurements were taken 10 days after tetracycline and cadmium were added to the reagent grade chlorophyll which was used at the concentration of chlorophyll in plants. Changes in absorption and fluorescence spectra are likely to result from removal of magnesium from the chlorophyll molecule, and thus they indicate the formation of pheophytin. Cadmium, on the other hand, is probably bound into the chlorophyll molecule, substituting its magnesium.

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Section: Absorption and Fluorescence Characteristics Of Chlorophyll Dsupporting

confidence: 74%

Section: Tetracycline Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chlorophyll degradation by tetracycline and cadmium in spinach (Spinacia oleracea L.) leaves

Rydzyński

Piotrowicz-Cieślak

Grajek

et al. 2018

Int. J. Environ. Sci. Technol.

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“…Quinolones have been widely used because of their ease of absorption, high drug levels in blood and lower cost amongst other advantages. CIP, originally developed by Bayer AG, has the basic features of fluoroquinolones, namely a broad antifungal spectrum and sterilizing effects . As for antimicrobial activity, CIP ranks first among all fluoroquinolones used in clinical practice .…”

Section: Introductionmentioning

confidence: 99%

“…CIP, originally developed by Bayer AG, has the basic features of fluoroquinolones, namely a broad antifungal spectrum and sterilizing effects. 2 As for antimicrobial activity, CIP ranks first among all fluoroquinolones used in clinical practice. 3 However, CIP causes antimicrobial resistance that increases with long-term use and storage in the body.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous separation, concentration and determination of trace fluoroquinolone antibiotics in environmental samples using a polymer aqueous two‐phase system coupled with HPLC

Sun

Zhu

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Fluoroquinolone antibiotics and their metabolites can accumulate in soil and water. Trace amounts of fluoroquinolone antibiotic residues in the environment necessitate the pretreatment of samples prior to analysis. In this work, a polyoxyethylene cetyl ether (POELE20)–NaH2PO4 aqueous two‐phase system (ATPS) was combined with high‐performance liquid chromatography (HPLC) to separate and determine trace quantities of ciprofloxacin (CIP), norfloxacin (NRF) and pefloxacin (PEF) in environmental samples. RESULTS Single‐factor, multi‐factor, reproducibility and accuracy experiments were performed. The extraction efficiencies and enrichment factors for the three antibiotics were above 90 and 13.8%, respectively, under optimal conditions. The recovery efficiency of CIP was 92.08–99.50%, with a relative standard deviation (RSD) of 0.60–6.34%, and the recovery efficiency of NRF and PEF was 93.40–99.30%, with RSD of 0.98–5.16%, when this method was applied to spiked water and soil samples. The RSDs of intra‐ and inter‐day analyses were 1.56–3.42 and 1.33–3.97%, respectively. CONCLUSIONS This study demonstrated that POELE20–NaH2PO4 ATPS coupled with HPLC was suitable for the simultaneous separation, concentration and determination of trace quantities of CIP, NRF and PEF in environmental samples. Moreover, this method can be applied for the determination of trace fluoroquinolone antibiotics in food samples. © 2019 Society of Chemical Industry

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UV‐A treatment of phenolic extracts impacts colour, bioactive compounds and antioxidant activity

Ebrahimi,

Hoxha,

Mihaylova

et al. 2024

J Sci Food Agric

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BACKGROUNDThe unintended co‐extraction of chlorophylls during the recovery of polyphenols from plant sources yields green‐coloured phenolic extracts with limited use in colour‐sensitive foods. This study aimed at decolourizing the ethanolic extracts of sugar beet leaves using a UV‐A treatment (390 nm).RESULTSExposure of the phenolic extracts to 30 UV‐A LEDs at 8.64 J m−2 radiation dose decreased the total chlorophyll content by 69.23% and reduced the greenness parameter (−a*) significantly (P < 0.05) from 27.33 ± 0.32 to 8.64 ± 0.16. Additionally, UV‐A treatment increased the content of most individual phenolic compounds (e.g. gallic acid, ferulic acid, etc.) significantly, resulting in an increase in the overall phenolic content in the extracts from 900.56 ± 14.11 μg g−1 fresh weight (FW) to a maximum of 975.09 ± 9.62 μg g−1 FW at 0.67 J m−2. However, rutin content had a significant decrease at the highest radiation dose (8.64 J m−2). The soluble sugar content (i.e. glucose and fructose) increased simultaneously with phenolic compounds after the UV‐A treatment. Although the UV treatment reduced the 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity, it had no significant effect on the ferrous chelating activity and the extract's ability to delay lipid oxidation in corn oil. The antioxidant activity index of the treated extract was comparable to that of butylated hydroxytoluene, a synthetic antioxidant.CONCLUSIONKey findings of this study include successful decolourization of the extract, decomposition of bound polyphenols to their free form, and maintaining the antioxidant activity of the extract in the oil system after UV‐A exposure. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Instability of chlorophyll in yellow lupin seedlings grown in soil contaminated with ciprofloxacin and tetracycline

Cited by 45 publications

References 39 publications

Chlorophyll degradation by tetracycline and cadmium in spinach (Spinacia oleracea L.) leaves

Chlorophyll degradation by tetracycline and cadmium in spinach (Spinacia oleracea L.) leaves

Simultaneous separation, concentration and determination of trace fluoroquinolone antibiotics in environmental samples using a polymer aqueous two‐phase system coupled with HPLC

UV‐A treatment of phenolic extracts impacts colour, bioactive compounds and antioxidant activity

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