Before gamma irradiation can be evaluated as a technique for preserving food in tin cans, it is necessary that the amount of radiation required for sterilization be known. Although information is available concerning the sensitivity of bacterial spores in buffers (Proctor and Goldblith, 1951; Lawrence et al., 1953), such conditions are quite different from those existing in tin cans. It therefore seemed desirable to study the effectiveness of gamma radiation for killing the spores of anaerobic, food-spoilage bacteria that were inoculated into meat packed in No. 2 tin cans; data presented here correlate the quantity of gamma radiation from cobalt-60 required to produce sterility with the numbers of such spores present in the meat. MATERIALS AND METHODS Three anaerobic, spore-forming bacteria were used in this study. Putrefactive anaerobe no. 3679 came from the National Canner's Association Research Laboratory, while Clostridium botulinum, strains 62A and 213B, were furnished by the Hooper Foundation for Medical Research at the University of California. Spore suspensions were prepared according to procedures described by Reed et al. (1951); putrefactive anaerobe no. 3679 was grown in pork infusion broth, but Difco bacto-casitone was substituted for casein digest in the medium, specified by these workers, for clostridia. Stock spore suspensions were suspended in sterile distilled water and stored at 4 C. Samples of these suspensions were tested for heat resistance by C. W. Bohrer of the National Canner's Association