Instructive Conductive 3D Silk Foam‐Based Bone Tissue Scaffolds Enable Electrical Stimulation of Stem Cells for Enhanced Osteogenic Differentiation

Hardy, John G.; Geissler, Sydney A.; Aguilar, David; Villancio-Wolter, Maria K.; Mouser, David J.; Sukhavasi, Rushi C.; Cornelison, Robert; Tien, Lee W.; Preda, Rucsanda C.; Hayden, Rebecca; Chow, Jacqueline K.; Nguy, Lindsey; Kaplan, David L.; Schmidt, Christine E.

doi:10.1002/mabi.201500171

Cited by 50 publications

(38 citation statements)

References 92 publications

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“…for bone repair (Hardy et al, 2015;Li et al, 2006;Park et al, 2010;Sofia et al, 2001;Zhang et al, 2014), for artificial skin (Sheikh et al, 2015), vascular grafts (Catto et al, 2015;Wang et al, 2015;Zhang et al, 2008), substrate for growing neuronal cells (Sun et al, 2015), IVD repair (Buser et al, 2011;Chang et al, 2010;Chen et al, 2015;Hu et al, 2012;Park et al, 2011;Zeng et al, 2014;Web Ref.1) (Table 2) and cartilage repair Hofmann et al, 2006a;Wang et al, 2005). Additionally, silk can also be used as a drug delivery platform (Mwangi et al, 2015;Qu et al, 2014) for antibiotics (Pritchard et al, 2013) and proteins or small molecule drugs (Meinel and Kaplan, 2012).…”

Section: Silk For Tissue Engineering Backgroundmentioning

confidence: 99%

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Frauchiger¹,

Tekari²,

Wöltje³

et al. 2017

eCM

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The degeneration of the intervertebral disc (IVD) within the spinal column represents a major pain source for many patients. Biological restoration or repair of the IVD using "compressive-force-resistant" and at the same time "cytocompatible" materials would be desirable over current purely mechanical solutions, such as spinal fusion or IVD implants. This review provides an overview of recent research on the repair of the inner (nucleus pulposus = NP) and the outer (annulus fibrous = AF) parts of the IVD tissue. Many studies have addressed NP repair using hydrogel-like materials. However, only a few studies have so far focused on AF repair. As the AF possesses an extremely low self-healing capacity and special attention to shear-force resistance is essential, special repair designs are required. In our review, we stated the challenges in IVD repair and highlighted the use of composite materials such as silk biomaterials and fibrin cross-linked reinforced hydrogels. We elaborated on the origin of silk and its many in tissue engineering. Furthermore, techniques such as electrospinning and 3D printing technologies allow the fabrication of versatile and functionalised 3D scaffolds. We summarised the research that has been conducted in the field of regenerative medicine over the recent years, with a special focus on the potential application and the potential of combining silk and reinforced -and thus mechanically tailored -hydrogels for IVD repair.

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Section: Silk For Tissue Engineering Backgroundmentioning

confidence: 99%

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Frauchiger¹,

Tekari²,

Wöltje³

et al. 2017

eCM

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“…Silk protein-based materials are also candidates for the generation of tissue scaffolds [24,25,26,27,28,29,30,31]. The natural silk fibroin of the domesticated Bombyx mori silkworm is the most commonly investigated for such applications [24,25,26,27,28,29,30,31,32]; however, recombinantly-produced silk-inspired proteins represent interesting alternatives because it is possible to produce large quantities of such silks with designed primary sequences [33,34,35,36,37].…”

Section: Introductionmentioning

confidence: 99%

“…The natural silk fibroin of the domesticated Bombyx mori silkworm is the most commonly investigated for such applications [24,25,26,27,28,29,30,31,32]; however, recombinantly-produced silk-inspired proteins represent interesting alternatives because it is possible to produce large quantities of such silks with designed primary sequences [33,34,35,36,37]. Silk-based composites are also widely investigated for application as tissue scaffolds [37,38,39,40], and preclinical trials in animal models are promising [35,36,41].…”

Section: Introductionmentioning

confidence: 99%

Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering

et al. 2016

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Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.

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“…The recognized ability of silk fibroin for the fabrication of scaffolds, both by aqueous and organic solvents, made it a special material in tissue engineering as it is one of the few FDA approved biomaterial [7]. Silk fibroin is widely used in biomedical applications both in the form of films [8] and 3D constructs [9], for different applications such as micropatterning [10] and microfluidics [11], implants [12] and drug delivery vehicles [13]. The ß-sheet content and morphology of silk fibroin can be controlled by different post processing techniques such as ethanol treatment /autoclaving and the duration of these treatments allows the modulation of the crystal composition, structure, mechanical properties and the degradation rate.…”

Section: Introductionmentioning

confidence: 99%

Physico-chemical and biological studies on three-dimensional porous silk/spray-dried mesoporous bioactive glass scaffolds

Chandrasekaran

Novajra

Carmagnola

et al. 2016

Ceramics International

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a b s t r a c tThe incorporation of a bioactive inorganic phase in polymeric scaffolds is a good strategy for the improvement of the bioactivity and the mechanical properties, which represent crucial features in the field of bone tissue engineering. In this study, spray-dried mesoporous bioactive glass particles (SD-MBG), belonging to the binary system of SiO 2 -CaO (80:20 mol%), were used to prepare composite scaffolds by freeze-drying technique, using a silk fibroin matrix. The physico-chemical and biological properties of the scaffolds were extensively studied. The scaffolds showed a highly interconnected porosity with a mean pore size in the range of 150 mm for both pure silk and silk/SD-MBG scaffolds. The elastic moduli of the silk and silk/SD-MBG scaffolds were 1.1 7 0.2 MPa and 6.9 7 1.0 MPa and compressive strength were 0.5 70.05 MPa and 0.9 70.2 MPa, respectively, showing a noticeable increase of the mechanical properties of the composite scaffolds compared to the silk ones. The contact angle value decreased from 105.3°to 71.2°with the incorporation of SD-MBG particles. Moreover, the SD-MBG incorporation countered the lack of bioactivity of the silk scaffolds inducing the precipitation of hydroxyapatite layer on their surface already after 1 day of incubation in simulated body fluid. The composite scaffolds showed good biocompatibility and a good alkaline phosphatase activity toward human mesenchymal stromal cells, showing the ability for their use as three-dimensional constructs for bone tissue engineering.

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Instructive Conductive 3D Silk Foam‐Based Bone Tissue Scaffolds Enable Electrical Stimulation of Stem Cells for Enhanced Osteogenic Differentiation

Cited by 50 publications

References 92 publications

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering

Physico-chemical and biological studies on three-dimensional porous silk/spray-dried mesoporous bioactive glass scaffolds

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