Instrumentation for noninvasive express-diagnostics bacteriophages and viruses by optical method

Study of a laser method to control the condition of coatings of titanium and magnesium alloys for medical cryo instruments

“…The spectral distributions characterizing pathogen solutions in water usually have sharp peaks. At low concentration, the logarithm of the intensity of the maximum Stokes and anti-Stokes components is usually a linear function of concentration [15]. If the peak was observed in the Stokes region, the results of the spectral analysis of the samples coincided with the results of standard tests.…”

“…The spectral distributions of solutions obtained from rubber surface washout on some samples had an additional third peak. The gauged method [15] was used to control virulence properties in parallel. The ratio of the logarithms of the intensities of the maxima of the Lorentzian components of the peak corresponding to the object and the peak corresponding to the sample for an object concentration of 10 3 cells \ ml for the optical method is presented in Table 1.…”

Detecting presence of live and dead pathogenic organisms in water and the water solutions by laser spectroscopy

“…However, the intensity is not an informative parameter, because it will depend on a power of the IR source (figure 3). At the same time, for the developed method the difference between the frequencies (wavelengths) of the maximum of the Lorenz component of a laser mode and the maximum of the Lorenz component of a Stokes component was found to be an informative parameter [4]. However, the Stokes component exists for no longer than 20 min in the spectrum.…”

“…We also used mixes of E. coli and latex particles with the same concentrations and the same respective ratio concentrations. Spectra of bacteria, unlike viruses [4] often have several maxima (figure 2). However, as a rule, only one peak has an intensity maximum at the same frequency in different solutions, such as water or physiological solution.…”

“…We selected the following model parameters: the peak positions of spectral lines corresponding to pathogens, and the difference between two wavelengths corre sponding, respectively, to the laser mode laser maximum, and to the peak positions of a spectral line of a pathogen. Previously, we studied the spectra of a virus [4]. The present work is devoted to the study of the spectra of bacteria.…”