Insulin binding of human and porcine monocomponent insulin to monocytes in Type 1 (insulin-dependent) diabetic patients and control subjects

Abstract

Summary. In the present study insulin binding properties of human semi-synthetic and porcine insulin were compared in Type 1 (insulin-dependent) diabetic patients treated from the onset of their disease either with human semi-synthetic insulin (n=12) or porcine insulin (n=12) and control subjects (n=12). In all three groups, insulin binding to circulating monocytes revealed no difference between human semi-synthetic and porcine insulin (specific insulin binding at tracer concentration: 5.7 + 0.5% versus 5.4_+ 0.5% in control subjects, 5.5 + 0.4% versus 5.4 + 0.4% in Type 1 diabetic patients on porcine insulin, 5.3 + 0.4% versus 5.6 + 0.4% in Type 1 diabetic patients on human insulin). Treatment with human or porcine insulin did not have any significant influence on receptor binding properties of the two diabetic groups investigated. Absolute receptor number and affinity of both diabetic groups were within the range of healthy control subjects irrespective of treatment with human or porcine insulin.