Insulin‐like growth factor‐1 (IGF‐1) promotes myoblast proliferation and skeletal muscle growth of embryonic chickens via the PI3K/Akt signalling pathway

Yu, Minli; Wang, Huan; Xu, Yali; Yu, Debing; Li, Dongfeng; Liu, Xiuhong; Du, Weihua

doi:10.1002/cbin.10466

Cited by 109 publications

(92 citation statements)

References 27 publications

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“…In this study, we observed that both USCs-EVs and iMSCs-EVs promoted C2C12 myoblast proliferation. Previous studies reported that VEGF, HGF and IGF-1 were not only potential proangiogenic factors but also promoted myoblast proliferation [53][54][55][56][57][58][59]. In the present study, we found that VEGF, HGF and IGF-1 were loaded in the USCs-EVs.…”

Section: Discussionsupporting

confidence: 67%

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Zhu

Niu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Our previous studies have shown that human urine-derived stem cells (USCs) have great potential as a cell source for cytotherapy and tissue engineering and that extracellular vesicles (EVs) secreted by USCs (USCs-EVs) can prevent diabetes-induced kidney injury in an animal model. The present study was designed to evaluate the effects of USCs-EVs on ischemia repair. Methods: USCs-EVs were isolated and purified by a battery of centrifugation and filtration steps. The USCs-EVs were then characterized by transmission electron microscopy, western blot and tunable resistive pulse sensing techniques. After intramuscularly transplanting USCs-EVs into an ischemic mouse hind-limb, we observed the therapeutic effects of USCs-EVs on perfusion by laser doppler perfusion imaging, angiogenesis and muscle regeneration by histology and immunohistochemistry techniques over 21 days. We subsequently tested whether USCs-EVs can induce the proliferation of a human microvascular endothelial cell line HMEC-1 and a mouse myoblast cell line C2C12 by cell counting kit 8 assay in vitro. Meanwhile, the potential growth factors in the USCs-EVs and supernatants of the USCs cultures were detected by enzyme-linked immunosorbent assay. Results: The USCs-EVs were spherical vesicles with a diameter of 30–150 nm and expressed exosomal markers, such as CD9, CD63 and Tsg101. Ischemic limb perfusion and function were markedly increased in the hind-limb ischemia (HLI) model after USCs-EVs administration. Moreover, angiogenesis and muscle regeneration levels were significantly higher in the USCs-EVs treatment group than in the PBS group. The in vitro experiments showed that USCs-EVs facilitated HMEC-1 and C2C12 cell proliferation in a dose-dependent manner. Conclusions: These results revealed for the first time that USCs-EVs efficiently attenuate severe hind-limb ischemic injury and represent a novel therapy for HLI.

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Section: Discussionsupporting

confidence: 67%

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Zhu

Niu

et al. 2018

Cell Physiol Biochem

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“…The ability of IGF-1 to accelerate cell viability was also found in the previous literature. A report declared that IGF-1 promoted myoblast proliferation [27]. Furthermore, Yan et al demonstrated that IGF-1 promoted cell proliferation of human periodontal ligament stem cells [28].…”

Section: Discussionmentioning

confidence: 99%

Insulin Growth Factor 1 Protects Neural Stem Cells Against Apoptosis Induced by Hypoxia Through Akt/Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase (Akt/MAPK/ERK) Pathway in Hypoxia-Ishchemic Encephalopathy

Zhao

Zheng

2017

Med Sci Monit

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BackgroundInsulin growth factor 1 (IGF-1) is reported to modulate cell growth and acts as potential therapy for traumatic brain injury. This study was designed to investigate the effect of IGF-1 on hypoxia-induced apoptosis in neural stem cells (NSCs).Material/MethodsA hypoxia model was constructed using NSCs separated from the hippocampus of rat. NSCs were divided into four groups: cells under normoxic conditions that acted as controls (C group), cells under hypoxia (H group), cells under hypoxia with IGF-1 (HI group), and cells under hypoxia with IGF-1 as well as picropodophyllin (PPP), which acts as an inhibitor of the IGF-1 receptor (HIP group). The cell viability and apoptosis were respectively measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. Finally, the phosphorylation levels of apoptosis-associated proteins and key kinases in the phosphatidylinositol-3-kinase (PI3K)/AKT and the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathways were detected by Western blot analysis.ResultsIn comparison with the H group, the cell viability was increased while the cell apoptosis was reduced by IGF-1 in the HI group. Besides, the expression levels of Bax, cytochrome c, and activated caspase-3 were all improved in the H group, and the remarkable differences were eliminated in the HI group compared with the C group. The expression level of Bcl-2 was the opposite. Additionally, down-regulations of phosphorylated AKT, MAPK, and ERK induced by hypoxia were all improved by IGF-1. All the influences of IGF-1 were weakened by addition of PPP.ConclusionsIGF-1 increased cell viability while decreasing apoptosis in hypoxic NSCs through the PI3K/AKT and MAPK/ERK pathways.

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“…; Yu et al . ). We were able to measure tarsus and wing length of nestlings, respectively, from days 7 and 8 post‐hatch onwards.…”

Section: Discussionmentioning

confidence: 97%

Causal link between insulin‐like growth factor 1 and growth in nestlings of a wild passerine bird

et al. 2016

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1. Life history of animals is strongly linked with the variability in postnatal growth rates, because the ability to achieve necessary body mass early in life is directly connected with the ability to survive and reproduce later in life. Surprisingly, little is known about the physiological mechanisms connecting food availability with the variability of growth in wild animals. 2. We used a hormonal injection treatment in free-living nestlings of the pied flycatcher (Ficedula hypoleuca). In doing this, we aimed to see how their plasma insulin-like growth factor 1 (IGF-1) levels and its mediation of growth rate vary in response to administration of exogenous IGF-1 during the early period of postnatal development.3. We showed that the levels of IGF-1 as well as growth rate and body size, in terms of body mass and tarsus length, were significantly higher in IGF-1-injected nestlings, compared with those in the control group. These differences became especially pronounced from day 7 posthatch onwards during the 13-day nestling period. Additionally, the proportional investment into growth was more profoundly downregulated in IGF-1-injected nestlings at the end of the nestling period, which achieved fledging size earlier, compared with control nestlings. 4. Our results are the first to highlight IGF-1 as the causal link between growth conditions and variability of growth rate and body size in wild vertebrates. Interestingly, the change in IGF-1 levels also showed a plausible, distinct adaptive age-and physical maturity-dependent pattern in nestlings to match the benefits gained by them when investing in growth, with the cost endured during the early days of postnatal development to maximize their fitness. Therefore, this mechanism might be among those mediating the maintenance of genetic variability in growth rates in populations, presumably regardless of the presence of strong directional selection acting upon them.

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Insulin‐like growth factor‐1 (IGF‐1) promotes myoblast proliferation and skeletal muscle growth of embryonic chickens via the PI3K/Akt signalling pathway

Cited by 109 publications

References 27 publications

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Insulin Growth Factor 1 Protects Neural Stem Cells Against Apoptosis Induced by Hypoxia Through Akt/Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase (Akt/MAPK/ERK) Pathway in Hypoxia-Ishchemic Encephalopathy

Causal link between insulin‐like growth factor 1 and growth in nestlings of a wild passerine bird

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