Insulin-like growth factor-1 protects SH-SY5Y cells against β-amyloid-induced apoptosis via the PI3K/Akt-Nrf2 pathway

Wang, Zigao; Xiong, Lize; Wang, Guanqun; Wan, Wenbin; Zhong, Chunjiu; Zu, Hengbing

doi:10.1016/j.exger.2016.11.009

Cited by 29 publications

(24 citation statements)

References 41 publications

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“…Several prior studies have demonstrated that activated IGF-1/IGF-1R axis could promote the nuclear translocation of Nrf2 that exerts multiple cellular effects against oxidative stress and apoptosis. [42][43][44] As expected, the increased nuclear expression of Nrf2 in hippocampal region was observed after 2 weeks exercise ( Figure 5A). To assess the role of IGF-1 signaling in Nrf2 activation in NPCs, aNPCs were treated with IGF-1 for 24 hours to analyze Nrf2 localization.…”

Section: Nrf2 Acts Downstream Of Igf-1 To Regulate Proteasome Activitysupporting

confidence: 83%

“…43,78 IGF-1 significantly promoted the nuclear translocation of Nrf2 and upregulated the expression of its downstream gene heme oxygenase-1 (HO-1) on Aβ 25-35 -induced apoptosis and ROS generation to protect SH-SY5Y cells. 42 Here, we demonstrated that IGF-1/ Nrf2 signaling is involved in exercise-elicited benefits through the upregulation of proteasome activity in hippocampus. Using IGF-1 signaling blocker leads to transcriptional repression of Nrf2, subsequently attenuating exercise-induced hippocampal proteasome activation.…”

Section: Discussionmentioning

confidence: 74%

“…As a downstream effector of IGF‐1, Nrf2 has been suggested to resist oxidative stress and β‐amyloid‐induced apoptosis in vivo and in vitro . IGF‐1 significantly promoted the nuclear translocation of Nrf2 and upregulated the expression of its downstream gene heme oxygenase‐1 (HO‐1) on Aβ 25‐35 ‐induced apoptosis and ROS generation to protect SH‐SY5Y cells . Here, we demonstrated that IGF‐1/Nrf2 signaling is involved in exercise‐elicited benefits through the upregulation of proteasome activity in hippocampus.…”

Section: Discussionmentioning

confidence: 83%

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Proteasome activation by insulin-like growth factor-1/nuclear factor erythroid 2-related factor 2 signaling promotes exercise-induced neurogenesis

et al. 2019

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Physical exercise-induced enhancement of learning and memory and alleviation of agerelated cognitive decline in humans have been widely acknowledged. However, the mechanistic relationship between exercise and cognitive improvement remains largely unknown. In this study, we found that exercise-elicited cognitive benefits were accompanied by adaptive hippocampal proteasome activation. Voluntary wheel running increased hippocampal proteasome activity in adult and middle-aged mice, contributing to an acceleration of neurogenesis that could be reversed by intrahippocampal injection of the proteasome inhibitor MG132. We further found that increased levels of insulin-like growth factor-1 (IGF-1) in both serum and hippocampus may be essential for exerciseinduced proteasome activation. Our in vitro study demonstrated that IGF-1 stimulated proteasome activity in cultured adult neural progenitor cells (NPCs) by promoting nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), followed by elevated expressions of proteasome subunits such as PSMB5. In contrast, pretreating adult mice with the selective IGF-1R inhibitor picropodophyllin diminished exercise-induced neurogenesis, concurrent with reduced Nrf2 nuclear translocation and proteasome activity.Likewise, lowering Nrf2 expression by RNA interference with bilateral intrahippocampal injections of recombinant adeno-associated viral particles significantly suppressed exercise-induced proteasome activation and attenuated cognitive function. Collectively, our work demonstrates that proteasome activation in hippocampus through IGF-1/Nrf2 signaling is a key adaptive mechanism underlying exercise-related neurogenesis, which may serve as a potential targetable pathway in neurodegeneration. K E Y W O R D Sadult neurogenesis, exercise, insulin-like growth factor-1, nuclear factor erythroid 2-related factor 2, neural progenitor cell, proteasome

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Section: Nrf2 Acts Downstream Of Igf-1 To Regulate Proteasome Activitysupporting

confidence: 83%

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 83%

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Proteasome activation by insulin-like growth factor-1/nuclear factor erythroid 2-related factor 2 signaling promotes exercise-induced neurogenesis

et al. 2019

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“…Gui, Liu, Shao, and Xu () found that another plant protein hydrolysate (cottonseed meal protein hydrolysate) replacing the cottonseed meal also improved the serum IGF‐1 in crucian carp ( Carassius auratus gibelio ). Study indicated that the IGF‐1 could promote antioxidant gene expressions through Nrf2 signalling pathway in human neuroblastoma SH‐SY5Y cells (Wang et al, ). This information suggested that ETSP might regulate antioxidant gene expressions through Nrf2 signalling pathway in fish intestine.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro biochemical studies have shown that ETSP can strongly remove radicals and inhibit lipid peroxidation (Moure, Domınguez, & Parajo, ; Sarmadi & Ismail, ). Recent research also indicated that ETSP improved the insulin‐like growth factor 1 (IGF‐1) of plasma in mice (Jayachandran & Xu, ), while the IGF‐1 could promote antioxidant gene expressions through Nrf2 signalling pathway in human neuroblastoma SH‐SY5Y cells (Wang et al, ). These appear that ETSP may be related to the Nrf2 signalling pathway in fish intestine to influence activities of antioxidant enzymes and relative gene expressions, which is valuable for investigation.…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary enzyme‐treated soy protein on the immunity and antioxidant status in the intestine of juvenile Jian carp ( Cyprinus carpio var. Jian)

et al. 2019

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This study was conducted to evaluate the hypothesis that enzyme‐treated soy protein (ETSP) can save the fish dietary protein, and further investigated the mechanism for saving effect by studying the effects of ETSP on intestinal immune response and antioxidant status in juvenile Jian carp (Cyprinus carpio var. Jian). Compared with the high‐protein diet, results showed that decreasing 2% of dietary protein increased the protein carbonyl (PC) content, decreased the anti‐hydroxyl radical capacity (AHR) and superoxide dismutase (SOD) activity and increased the relative expressions of pro‐inflammatory cytokine (p < 0.05). The low‐protein diet might impair the intestinal health, so as to reduce fish growth performance. After adding 1.5% or 2% of ETSP to the low‐protein diet, results indicated that (1) ETSP decreased the relative expressions of pro‐inflammatory cytokine and increased the relative expressions of anti‐inflammatory cytokine (p < 0.05). (2) ETSP decreased malondialdehyde (MDA) and PC content (p < 0.05), improved activities of antioxidant enzymes, glutathione (GSH) content, relative expressions of antioxidant enzymes and Nrf2, which were even much higher than the high‐protein diet (p < 0.05). All the above data suggested that optimal level of ETSP can save 2% of fish dietary protein which might be partly attributed to improve intestinal health through regulating intestinal immunity response and antioxidant status.

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RETRACTED: NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway

Jiao

2019

FEBS Open Bio

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As one of the main neurotrophic factors, nerve growth factor (NGF) participates in various processes related to viability, plasticity, and neuronal growth. NGF is known to protect against cell death and toxicity triggered by β‐amyloid (Aβ), but the underlying mechanism remains unclear. Here, we investigated this process in SKNSH neuroblastoma, in which NGF reduced cell death induced by Aβ25–35. Furthermore, NGF suppressed the production of reactive oxygen species (ROS) and promoted antioxidant function via Aβ25–35. Additionally, we demonstrated that NGF impaired the activation of the JNK/c‐Jun signaling pathway and significantly increased Nrf2 nuclear translocation and HO‐1 expression. Nrf2 elimination abolished the protective effect of NGF‐1 on Aβ25–35‐induced ROS generation, apoptosis, and activation of the JNK/c‐Jun pathway. The results of our study indicate that NGF protects neuroblastoma against injury triggered by Aβ25–35 via suppression of ROS–JNK/c‐Jun pathway stimulation through the Nrf2/HO‐1 pathway.

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Insulin-like growth factor-1 protects SH-SY5Y cells against β-amyloid-induced apoptosis via the PI3K/Akt-Nrf2 pathway

Cited by 29 publications

References 41 publications

Proteasome activation by insulin-like growth factor-1/nuclear factor erythroid 2-related factor 2 signaling promotes exercise-induced neurogenesis

Proteasome activation by insulin-like growth factor-1/nuclear factor erythroid 2-related factor 2 signaling promotes exercise-induced neurogenesis

Effect of dietary enzyme‐treated soy protein on the immunity and antioxidant status in the intestine of juvenile Jian carp ( Cyprinus carpio var. Jian)

RETRACTED: NGF protects neuroblastoma cells against β‐amyloid‐induced apoptosis via the Nrf2/HO‐1 pathway

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