Insulin-like Growth Factor-I and Growth Differentiation Factor-5 Promote the Formation of Tissue-Engineered Human Nasal Septal Cartilage

Alexander, Thomas; Sage, August B.; Chen, Albert C.; Schumacher, Barbara L.; Shelton, Elliot; Masuda, Koichi; Sah, Robert L.; Watson, Deborah

doi:10.1089/ten.tec.2009.0396

Cited by 39 publications

(53 citation statements)

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“…GDF-5 is involved in maintenance, growth and repair of bones, cartilage and musculoskeletal soft tissues including tendon [135][136][137]. When GDF-5 was first investigated for its possible role in tendon biology, it was found to possess a unique ability to induce a tendon-like tissue rather than cartilage and bone, when implanted intra-muscularly in rats [138].…”

Section: Functionmentioning

confidence: 99%

Genetic Variation, Protein Composition and Potential Influences on Tendon Properties in Humans

Foster¹

2012

TOSMJ

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Sequence variations in genes that code for proteins involved in homeostatic processes within tendons may influence tendon mechanical properties. Since variants of the four genes COL5A1, TNC, MMP3 and GDF5 have been implicated in the aetiopathogenesis of tendinopathies, which is ultimately characterised by abnormal structural and regulatory processes, sequence variations in these four genes may also influence how the tendon functions mechanically, even in the absence of tendinopathy. For example, two reports of association between variation in the COL5A1 gene and measures of flexibility complement reported associations between genotype and incidence of tendinopathy. Non-genetic factors such as age, body mass and physical activity status influence risk of tendon injury and physical performance potential independently from genomics, and also in gene-environment interactions. However, these non-genetic factors are often not considered in genetic association studies, probably due to their retrospective nature. Further research examining COL5A1, TNC, MMP3 and GDF5, as well as other genes that may influence the maintenance of tendon homeostasis such as COL1A1 which regulates the production of collagen type 1, the most abundant structural component of tendon is encouraged. Establishing the genetic basis of tendon properties in asymptomatic populations may advance understanding of some aspects relevant to physical performance and of the aetiology of tendinopathies. To improve understanding, accurate and reproducible assessments of tendon properties are required. However, no valid and reliable assessments of tendon properties, such as those involving in vivo ultrasound imaging techniques, have yet been applied to genetic association studies in humans.

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Section: Functionmentioning

confidence: 99%

Genetic Variation, Protein Composition and Potential Influences on Tendon Properties in Humans

Foster¹

2012

TOSMJ

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“…The cartilage from these donors was dissected free of perichondrium and diced into pieces (1 mm 3 ). The fragments were digested as reported previously [5]. Suspensions of digested cartilage were filtered (70 mm), then washed and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, this can be further limited by trauma, congenital deformities, or iatrogenic septal defects. Previous studies have successfully produced tissue engineered autologous neocartilage that may eventually be used for reconstructive surgery [5-7]. …”

Section: Introductionmentioning

confidence: 99%

“…However, there are no existing studies demonstrating the production of clinically relevant septal neocartilage constructs of large size. The goal of this investigation is to apply previous culture methods to the production of 24mm constructs [5,6]. Structural, biochemical, and biomechanical properties of neocartilage constructs will be evaluated and compared to those of standard 12mm constructs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

10.4172/2324-8785.1000172

et al. 2014

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Importance Cartilaginous craniofacial defects range in size and autologous cartilaginous tissue is preferred for repair of these defects. Therefore, it is important to have the ability to produce large size cartilaginous constructs for repair of cartilaginous abnormalities. Objectives To produce autologous human septal neocartilage constructs substantially larger in size than previously produced constructs To demonstrate that volume expanded neocartilage constructs possess comparable histological and biochemical properties to standard size constructs To show that volume expanded neocartilage constructs retain similar biomechanical properties to standard size constructs Design Prospective, basic science Setting Laboratory Participants The study used remnant human septal specimens removed during routine surgery at the University of California, San Diego Medical Center or San Diego Veterans Affairs Medical Center. Cartilage from a total of 8 donors was collected. Main Outcomes Measured Human septal chondrocytes from 8 donors were used to create 12mm and 24mm neocartilage constructs. These were cultured for a total of 10 weeks. Photo documentation, histological, biochemical, and biomechanical properties were measured and compared. Results The 24mm diameter constructs were qualitatively similar to the 12mm constructs. They possessed adequate strength and durability to be manually manipulated. Histological analysis of the constructs demonstrated similar staining patterns in standard and volume expanded constructs. Proliferation, as measured by DNA content, was similar in 24mm and 12mm constructs. Additionally, glycosaminoglycan (GAG) and total collagen content did not significantly differ between the two construct sizes. Biomechanical analysis of the 24mm and 12mm constructs demonstrated comparable compressive and tensile properties. Conclusion and Relevance Volume expanded human septal neocartilage constructs are qualitatively and histologically similar to standard 12mm constructs. Biochemical and biomechanical analysis of the constructs demonstrated equivalent properties. This study shows that modification of existing protocols is not required to successfully produce neocartilage constructs in larger sizes for reconstruction of more substantial craniofacial defects. Level of Evidence NA.

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“…A pesar de ser un recurso limitado en cuanto a su extensión finita y configuración geométrica particular, el cartílago septal ha sido el más comúnmente utilizado, debido a sus características estructurales y accesibilidad 22,23 . Por ello, la ingeniería tisular puede resultar una alternativa, con la posibilidad de originar una mayor cantidad de cartílago con la forma deseada.…”

Section: Cirugía Plástica Y Reconstructivaunclassified