Insulin-like growth factor-I gene polymorphism associations with growth, body composition, skeleton integrity, and metabolic traits in chickens

Abstract

Molecular genetic selection on individual genes is a promising method to genetically improve economically important traits in chickens. A resource population was developed to study the genetics of growth, body composition, skeletal integrity, and metabolism traits. Broiler sires were crossed to dams of 2 diverse, highly inbred lines (Leghorn and Fayoumi), and the F1 birds were intermated by dam line to produce broiler-Leghorn and broiler-Fayoumi F2 offspring. Growth, body composition, skeletal integrity, and hormonal and metabolic factors were measured in 713 F2 individuals. Insulin-like growth factor-I (IGF1) was selected for study as a biological and positional candidate gene. A single nucleotide polymorphism (SNP) was identified between the founder lines in the IGF1 promoter region, and a PCR-RFLP assay was developed. A mixed model was used to statistically analyze associations of IGF1-SNP1 with phenotypic traits. The IGF1-SNP1 had significant associations with most recorded traits, except metabolic traits. Strong interactions between the IGF1 gene and genetic background on growth traits in the 2 F2 populations suggest that genetic interaction is an important aspect for consideration before using the IGF1-SNP1 in marker-assisted selection programs. Several beneficial effects (improved growth, increased breast muscle weight, decreased abdominal fat, and enhanced skeletal integrity) associated with 1 allele indicate the presence of 1 or more loci near IGF1-SNP1 controlling biologically diverse and economically important traits in chickens.

Association of Insulin-Like Growth Factor-1 Gene Polymorphism at 279 Position of the 5’UTR Region with Body Weight Traits in Broiler Chicken

“…Sci., 9 (4): 213-222, 2015 weights of males were higher than those of females. These results are disagree with the results of Zhou et al (2005) who indicated that IGF-I gene polymorphism was associated with growth, body composition, skeleton integrity and metabolic traits in chickens. Also, disagree with the results of Gouda and Essawy (2010) who analyzed the polymorphism of IGF-I gene among Egypt chicken breeds and indicated their effect on the growth traits of Table 3 chicken was significant.…”

“…Amills et al (2003) found suggestive associations (p#0.05) between IGF1-SNP and average daily gain at 107 days. Zhou et al (2005) found in the F2 generation of hybrids Leghorn×broiler Fayoum×broiler, a significant association (at 5% significance level) between IGF1-SNP and average daily gains. Some results of research on the IGF-1 gene polymorphism is associated with growth have been reported in chickens (Sco et al, 2001;Kita et al, 2005;Li et al, 2009), in sheep (Zhang et al, 2008) and in cattle (Curi et al, 2006;Siadkowska et al, 2006;Arman et al, 2012).…”

“…Amills et al (2003) found suggestive associations between IGF1-SNP and average daily gain at 107 days. Zhou et al (2005) found in the F2 generation of hybrids Leghorn × broiler Fayoum×broiler, a significant association between IGF1-SNP and average daily gains. Fang et al (2008) found a significant correlation between IGF1 polymorphism and egg production.…”

“…Recent researches on polypeptides growth factors have identified several growth factors such as IGFs, epidermal growth factor, transforming growth factor and platelet-derived growth factor as modulators of muscle (Florini et al, 1996;Duclos, 1998). The Insulin-like Growth Factor Gene (IGF1) is a candidate gene for growth, body composition and metabolism, skeletal characteristics and growth of adipose tissue and fat deposition in chickens (Zhou et al, 2005) and Siddiqui et al (1992) reported a positive correlation between IGF-1 concentration and body weight. Nagaraja et al (2000) studied the association between feed intake, body weight, laying rate and egg weight with IGF-1 polymorphism and reported that it was possible to improve the economic traits by IGF-1 genotypes in an unselected population.…”

Identification of quantitative trait loci associated with bone traits and body weight in an F2 resource population of chickens

“…These studies have focused on identifying QTL responsible for body weight [39,46,47,51,52,55], feed-efficiency [57], growth [7,20,27,55,57,62], carcass characteristics [12,23,24,56], and egg traits [27,39,44,54,59]. Other researchers have investigated specific candidate genes potentially associated with variation in traits relating to bone integrity [31,61]. However, this is the first report of a genome scan focused on the identification of QTL influencing bone traits in chickens.…”