Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture

Schams, D.; Koll, R.; Li, C. H.

doi:10.1677/joe.0.1160097

Cited by 75 publications

(31 citation statements)

References 17 publications

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“…The increase in CYP11A1 and HSD3B1 stimulated by IGF1 may increase progesterone production per se, as well as providing precursor for OE 2 production. In accord with this, both Schams et al (1988) and Ryan et al (2008) found that addition of IGF1 increased granulosa cells' progesterone production. A similar action of IGF1 may therefore be of particular importance in vivo after the LH surge when follicles are starting to luteinize.…”

Section: Discussionsupporting

confidence: 54%

IGF1 induces up-regulation of steroidogenic and apoptotic regulatory genes via activation of phosphatidylinositol-dependent kinase/AKT in bovine granulosa cells

Mani¹,

Fenwick²,

Cheng³

et al. 2010

Reproduction

144

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IGF1, a potent stimulator of cellular proliferation, differentiation and development, regulates granulosa cell steroidogenesis and apoptosis during follicular development. Depending upon species and stage of follicular growth, IGF1 acts on granulosa cell steroidogenesis either alone or together with FSH. We examined the mechanism of action of IGF1 in bovine granulosa cells in serum-free culture without insulin to determine its potential role in the regulation of steroidogenic and apoptotic regulatory gene expression and to investigate the interaction of FSH with IGF1 on this mechanism. Bovine granulosa cells treated with IGF1 demonstrated a significant increase in 17b-oestradiol (OE 2 ) production, cell number and in mRNA expression of CYP11A1, HSD3B1, CYP19A1, BAX, type 1 IGF receptor (IGF1R) and FSHR, while FSH alone had no significant effects. IGF1 or FSH alone or both together had no effect on BCL2 expression. IGF1 with FSH resulted in a synergistic increase in granulosa cell number and in mRNA expression of CYP19A1 and IGF1R without altering OE 2 production. IGF1 stimulated the phosphoinositide 3 0 -OH kinase (PI3K) but not the MAPK pathway in granulosa cells, as evidenced by increased phosphorylation of AKT but not extracellularregulated kinase 1/2. Addition of the PI3K pathway inhibitor LY294002 (but not the MAPK pathway inhibitor PD98059) abrogated the increased expression of genes induced by IGF1. IGF1 therefore up-regulates the steroidogenic and apoptotic regulatory genes via activation of PI3K/AKT in bovine granulosa cells. The synergistic action of IGF1 with FSH is of likely key importance for the development of small antral follicles before selection; subsequently, other factors such as LH may also become necessary for continued cell survival.

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Section: Discussionsupporting

confidence: 54%

IGF1 induces up-regulation of steroidogenic and apoptotic regulatory genes via activation of phosphatidylinositol-dependent kinase/AKT in bovine granulosa cells

Mani¹,

Fenwick²,

Cheng³

et al. 2010

Reproduction

144

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show abstract

“…This is similar to what has been observed in granulosa cells of sheep follicles of similar size (Monniaux & Pisselet 1992). However, granulosa cells from more developed follicles respond to IGF1 with increased progesterone production (Monniaux & Pisselet 1992), and granulosa cells from other species also responded with increased progesterone production (Schams et al 1988, Yong et al 1992, Shaw et al 1993, Armstrong et al 1996, Spicer & Aad 2007. Thus, IGFs could affect progesterone production in preovulatory follicles in the brushtail possum.…”

Section: Follicle Typesupporting

confidence: 85%

The role of IGFs in the regulation of ovarian follicular growth in the brushtail possum (Trichosurus vulpecula)

Juengel

Haydon²,

Mester³

et al. 2010

Reproduction

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IGFs are known to be key regulators of ovarian follicular growth in eutherian mammals, but little is known regarding their role in marsupials. To better understand the potential role of IGFs in the regulation of follicular growth in marsupials, expression of mRNAs encoding IGF1, IGF2, IGF1R, IGF-binding protein 2 (IGFBP2), IGFBP4 and IGFBP5 was localized by in situ hybridization in developing ovarian follicles of the brushtail possum. In addition, the effects of IGF1 and IGF2 on granulosa cell function were tested in vitro. Both granulosa and theca cells synthesize IGF mRNAs, with the theca expressing IGF1 mRNA and granulosa cell expressing IGF2 mRNA. Oocytes and granulosa cells express IGF1R. Granulosa and theca cells expressed IGFBP mRNAs, although the pattern of expression differed between the BPs. IGFBP5 mRNA was differentially expressed as the follicles developed with granulosa cells of antral follicles no longer expressing IGFBP5 mRNA, suggesting an increased IGF bioavailability in the antral follicle. The IGFBP protease, PAPPA mRNA, was also expressed in granulosa cells of growing follicles. Both IGF1 and IGF2 stimulated thymidine incorporation but had no effect on progesterone production. Thus, IGF may be an important regulator of ovarian follicular development in marsupials as has been shown in eutherian mammals.

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“…Einspanier et al (28) observed that the concentration of IGF-I in the tissue of the bovine corpus luteum increased during the development of the corpus luteum, peaked during the late luteal phase, and decreased at luteolysis. IGF-I can induce steroidogenesis in thecal cells (97,105), granulosa cells (93,98), and corpus luteum (71). Cows that are in positive energy balance have higher concentrations of IGF-I in the blood (62,100) and secrete more progesterone during the luteal phase than do cows that are in NEB (100).…”

Section: Relationship Between Negative Energy Ba-lance and Reproductionmentioning

confidence: 99%

Relationship between overfeeding and overconditioning in the dry period and the problems of high producing dairy cows during the postparturient period

Rukkwamsuk

Kruip²,

Wensing

1999

Veterinary Quarterly

147

106

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Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture

Cited by 75 publications

References 17 publications

IGF1 induces up-regulation of steroidogenic and apoptotic regulatory genes via activation of phosphatidylinositol-dependent kinase/AKT in bovine granulosa cells

IGF1 induces up-regulation of steroidogenic and apoptotic regulatory genes via activation of phosphatidylinositol-dependent kinase/AKT in bovine granulosa cells

The role of IGFs in the regulation of ovarian follicular growth in the brushtail possum (Trichosurus vulpecula)

Relationship between overfeeding and overconditioning in the dry period and the problems of high producing dairy cows during the postparturient period

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