Insulin receptor binding and tyrosine kinase activity in liver and skeletal muscle from fasted rats

Balage, Michèle; Sornet, Claire; Manin, M.; Grizard, Jean

doi:10.1051/rnd:19890607

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…Furthermore, quantification of the autoradiograms showed that the magnitude of the increase in the level of insulinreceptor mRNA in skeletal muscle was greater than 2-fold. This is comparable with the changes in the amount of insulin-receptor protein that has previously been observed in response to similar fasting conditions (Balage et al 1989). The results suggest, therefore, that receptor number may be controlled at a pretranslational level, i.e.…”

Section: Discussionsupporting

confidence: 89%

“…A change in the level of mRNA is a general indication of pretranslational regulation, but it does not distinguish between alterations in mRNA stability or alterations in gene transcription (Nielson & Shapiro, 1990). Since insulin sensitivity and receptor number are modified in fasting (Brady et al 1981, Balage et al 1989), it appears probable that the observed changes in insulinreceptor mRNA levels are reflected in the production of an active receptor.…”

Section: Discussionmentioning

confidence: 99%

“…fasting (Issad et al 1987), the number of insulin receptors present in muscle and the liver increases in fasted animals (Balage et al 1989), while the tyrosine kinase activity of the receptors is normal in muscle but decreased in liver (Balage et al 1989;Contreras et a/. 1990).…”

mentioning

confidence: 99%

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Changes in insulin-receptor mRNA levels in skeletal muscle and brown adipose tissue of weanling rats during fasting and refeeding

Knott¹,

Trayhurn²,

Hesketh³

1992

Br J Nutr

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Tissue-specific alterations in insulin sensitivity occur in response to fasting and refeeding, as part of the integrated adaptive mechanisms employed to adjust to major changes in nutritional status. In the present study the effects of fasting and refeeding on insulin-receptor, actin and myosin mRNA levels in skeletal muscle, and insulin-receptor and uncoupling-protein mRNA in brown adipose tissue of rats have been examined. Insulin-receptor mRNA levels increased markedly in both skeletal muscle and brown adipose tissue after a 40 h fast, the increase being greater in brown fat (8-fold) than in muscle (2-fold). On refeeding for 4 h, the insulin-receptor mRNA level in both tissues declined rapidly to control levels. An increase in insulin-receptor mRNA level was also observed in brown adipose tissue after a 16 h fast, although not in skeletal muscle. In contrast to the insulin-receptor mRNA, the level of the mRNA for the mitochondria1 uncoupling protein declined markedly in brown adipose tissue during a 40 h fast. These results indicate that insulin-receptor mRNA levels are modulated in response to the alterations in nutritional status that occur during fasting and refeeding; this may reflect a nutritional influence on transcription of the receptor-protein gene.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Changes in insulin-receptor mRNA levels in skeletal muscle and brown adipose tissue of weanling rats during fasting and refeeding

Knott¹,

Trayhurn²,

Hesketh³

1992

Br J Nutr

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“…Although changes in insulin receptor binding characteristics have been documented with fasting (Balage et al, 1989;, there is little information on GR binding characteristics. Preliminary results from our laboratory indicated a glucagon receptor binding capacity 2.6 times greater in purified liver Can.…”

Section: Glucagon Receptormentioning

confidence: 99%

Glucagon Receptors: Effect of Exercise and Fasting

Lavoie

2005

Can. J. Appl. Physiol.

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One paradox of hormonal regulation during exercise is the maintenance of glucose homeostasis after endurance training despite a lower increase in plasma glucagon. One explanation could be that liver sensitivity to glucagon is increased by endurance training. Glucagon exerts its effect through a 62 KDa glycoprotein receptor, member of the G protein-coupled receptor. To determine whether changes with exercise in glucagon sensitivity occurred at the level of the glucagon receptor (GR), binding characteristics of hepatic glucagon receptors were ascertained in rat purified plasma membranes. Saturation kinetics indicated no difference in the dissociation constant or affinity of glucagon receptor, but a significantly higher glucagon receptor binding density in liver in endurance trained compared to untrained animals. Along with endurance training, it appears that fasting also changes GR binding characteristics. In animals fasting 24 hrs, a significant increase in glucagon receptor density was also reported. Although the exact mechanism remains unknown, there is no doubt that the liver can adapt to physiological stress through modulation of GR binding characteristics to enhance the hepatic glucose production responsiveness to glucagon.

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Alterations in the level of insulin receptor and GLUT-4 mRNA in skeletal muscle from rats fed a kidney bean (Phaseolus vulgaris) diet

Knott¹,

Grant²,

Bardócz³

et al. 1992

International Journal of Biochemistry

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Insulin receptor binding and tyrosine kinase activity in liver and skeletal muscle from fasted rats

Cited by 3 publications

References 5 publications

Changes in insulin-receptor mRNA levels in skeletal muscle and brown adipose tissue of weanling rats during fasting and refeeding

Changes in insulin-receptor mRNA levels in skeletal muscle and brown adipose tissue of weanling rats during fasting and refeeding

Glucagon Receptors: Effect of Exercise and Fasting

Alterations in the level of insulin receptor and GLUT-4 mRNA in skeletal muscle from rats fed a kidney bean (Phaseolus vulgaris) diet

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