Insulin receptors: the binding capacity and localization in the digestive tract during the rabbits neonatal period

Nowak, J; Styczyńska, E; Ab, Slebodziński

doi:10.1051/rnd:19960507

Cited by 5 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The general decrease in B max of IGF-IIR possibly reflected a decreasing postnatal role of IGF-II as shown in Table 3. rats (Nissley et al 1993, Breier et al 2000. The overall rise with age of B max of IR in the small intestine, which was especially marked in the ileum from day 1 to day 8 of life, suggests involvement of IR in small intestine development (Nowak et al 1996, Odle et al 1996, Buts et al 1997 and, in calves, especially in the ileum .…”

Section: Changes Of Igf-ir Igf-iir and Ir At The Protein And Mrna Lementioning

confidence: 89%

“…Studies on site-specific and on postnatal variations of intestinal IGF-IR, IGF-IIR and IR have been reported in pigs, rabbits and rats (Schober et al 1990, Young et al 1990, Nowak et al 1996, Staley et al 1998. Differences in IGF and insulin receptor numbers at different intestinal sites as found in our calves have been reported in rats (Heinz-Erian et al 1991, Buts et al 1997, FernandezMoreno et al 1997 and are consistent with our previous studies in calves (Baumrucker et al 1994).…”

Section: Igf-ir Igf-iir and Ir Concentrations At The Mrna And Proteimentioning

confidence: 99%

“…Thus, these receptors exhibited ontogenetic differences. Because IGF-IR and IR numbers in piglets and rabbits were highest at birth and then declined for 3-6 days (Schober et al 1990, Nowak et al 1996, there are obviously species differences with respect to the ontogenetic behavior of B max of IGF-IR and IR. The general decrease in B max of IGF-IIR possibly reflected a decreasing postnatal role of IGF-II as shown in Table 3. rats (Nissley et al 1993, Breier et al 2000.…”

Section: Changes Of Igf-ir Igf-iir and Ir At The Protein And Mrna Lementioning

confidence: 99%

See 2 more Smart Citations

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

Georgiev¹,

Georgieva²,

Pfaffl³

et al. 2003

Journal of Endocrinology

View full text Add to dashboard Cite

Intestinal development is modified by age and nutrition, mediated in part by insulin-like growth factors (IGF-I, IGF-II) and insulin. We have investigated whether expression of IGF-I, IGF-II and insulin receptors (IGF-IR, IGF-IIR and IR; measured by real-time RT-PCR) and binding capacity (B max ) of IGF-IR, IGF-IIR and IR in the mucosa of the small and large intestine of neonatal calves are modified by age and different feeding regimes.In experiment 1, pre-term (GrP) and full-term (GrN) calves (after 277 and 290 days of pregnancy respectively) were killed immediately after birth before being fed; a further group of full-term calves were fed for 7 days and killed on day 8 of life (GrC 1-3 ). In experiment 2, full-term calves were killed on day 8 after being fed first-colostrum for 7 days (GrC max

show abstract

Section: Changes Of Igf-ir Igf-iir and Ir At The Protein And Mrna Lementioning

confidence: 89%

Section: Igf-ir Igf-iir and Ir Concentrations At The Mrna And Proteimentioning

confidence: 99%

Section: Changes Of Igf-ir Igf-iir and Ir At The Protein And Mrna Lementioning

confidence: 99%

See 1 more Smart Citation

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

Georgiev¹,

Georgieva²,

Pfaffl³

et al. 2003

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Receptors for insulin and IGF-I are present in the gastrointestinal tract of many species [4][5][6] , and in the intestinal mucosa of young animals, which have a higher affi nity for insulin and IGF-I than adult mucosa [4,18] . Although the affi nity of IGF-I receptors for insulin is much lower than their affi nity for IGF-I, the insulin level in the milk used in our study was many times higher than that in normal milk, which possibly led to considerable amounts of insulin binding to IGF-I receptors.…”

Section: Discussionmentioning

confidence: 99%

Dietary Insulin Affects Leucine Aminopeptidase, Growth Hormone, Insulin-Like Growth Factor I and Insulin Receptors in the Intestinal Mucosa of Neonatal Pigs

Huo

Macdonald

et al. 2006

Neonatology

View full text Add to dashboard Cite

Background: Recent studies suggest that milk-borne insulin may regulate the development of the gastrointestinal tract in neonatal mammals. Objectives: To explore the mechanism by which milk-borne insulin affects gastrointestinal tract development, we examined the effect of dietary insulin on the expression levels of leucine aminopeptidase (LAP) and insulin-like growth factor I (IGF-I), as well as its effect on growth hormone (GH), IGF-I and insulin receptors in the small intestinal mucosa of neonatal pigs. Methods: Five piglets were anesthetized and sampled within 2–4 h after birth. They were not allowed to suckle and were used as newborn controls (group N). Ten other piglets from 5 litters were randomly divided into group M (n = 5), which was fed cow’s milk, and group MI (n = 5), which was fed cow’s milk and insulin (2.5 mg/l). Piglets in groups M and MI were artificially fed for 3 days and then sampled. Total RNA in their intestinal mucosa was extracted with Tripure reagents (Roche, USA). Reverse transcription PCR (RT-PCR) was used to semi-quantify mRNA levels of target genes and 18S rRNA was used in an RT-PCR system as an internal control. PCR products were loaded onto a 9% nondenaturing polyacrylamide gel. The gel was stained by silver staining agents. Digital photos were taken and the strength of the band areas was quantified using software. Results: The results showed that the DNA contents and LAP activity in the small intestines of the piglets in group MI were higher (p < 0.05) than in the piglets in group N. Compared with group M, piglets in group MI exhibited significantly increased expression levels of both insulin and GH receptor in the ileum, and LAP in the jejunum (p < 0.05); IGF-I receptor expression levels in both the jejunum and ileum were significantly decreased (p < 0.01 and p < 0.05, respectively), while IGF-I expression was unchanged (p > 0.05). Conclusion: Collectively, dietary insulin increased mRNA levels of insulin and GH receptor, which could help explain the effect of dietary insulin on receptor-mediated postnatal development of the small intestine. Dietary insulin suppressed IGF-I receptor expression, which may be the result of negative feedback caused when insulin binds to IGF-I receptors.

show abstract

Chapter 7 Roles of milk-borne components in the development of pancreatic enzymes during the suckling period

Kinouchi¹,

Yajima²

2002

Biology of Growing Animals

View full text Add to dashboard Cite

Insulin receptors: the binding capacity and localization in the digestive tract during the rabbits neonatal period

Cited by 5 publications

References 13 publications

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

Dietary Insulin Affects Leucine Aminopeptidase, Growth Hormone, Insulin-Like Growth Factor I and Insulin Receptors in the Intestinal Mucosa of Neonatal Pigs

Chapter 7 Roles of milk-borne components in the development of pancreatic enzymes during the suckling period

Contact Info

Product

Resources

About