Insulin-Regulatory Mechanisms and Diabetes Mellitus

Diffusion of Glucose, Insulin, Inulin, and Evans Blue Protein into Thoracic Duct Lymph of Man*

“…Few in vitro investigations have attempted to elucidate the nature of plasma insulin; there is some indirect evidence that most of the circulating IRI exists as a relatively small molecule (1-3), whereas the fraction of plasma ILA immunologically inactive may be composed of one or more species of larger molecules, obtained either by polymerization of a smaller unit (4) or by binding to a carrier protein (5).…”

Failure to Demonstrate “Bound” Insulin in Human Serum

“…These investigators also assumed that the fat pad was able to dissociate the complex and therefore measured both the "free" and "bound" form. 10 " 12 Antoniades et. al.…”

Extrapancreatic and Intrapancreatic Action of Antidiabetic Sulphonylureas a Review

“…Details of these experiments have not been published. Lacy (65 ) reported that tolbutamide added in vitro at a concentration of 100 mg per cent increased the ILA (fat pad technique) of sera from Antoniades et al ( 6 ) have examined the content of "free" and "bound insulin" in the blood of non-diabetic and non-insulin dependent diabetics before and after intravenous injection of tolbutamide. In 7 out of 13 non-diabetic cases they observed a pronounced increase in the concentration of "free" and a fall in the concentration of "bound insulin" after the tolbutamide injection.…”