Insulinbindende Antikörper und Insulinbedarf bei Diabetikern

Kerp, L.; Kasemir, H.; Kieling, F.

doi:10.1007/bf01734130

Cited by 20 publications

(5 citation statements)

References 27 publications

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“…No relationship was found between daily insulin dose, the quality of diabetic control, serum free insulin concentrations and insulin antibody binding con¬ stants, these inter-relationships have previously been the subject of many con¬ flicting studies (Kerp et al 1968;Hurn et al 1969;Dixon et al 1975;Lev-Ran 8c Rat 1973;Heding 1972;Nakagawa et al 1973;Lewis et al 1976). However, it has to be emphasized that studies of this nature are inevitably complicated by difficulties of assessing the quality of diabetic control (Malone et al 1976;Alberti et al 1975), in equating insulin dose with insulin requirement (Yalow 8c Berson 1961;Watkins et al 1967) and with methodological differences in the quantitative assessment of insulin antibodies (Hurn et al 1969;Dunlop 8c Court 1974).…”

Section: Discussionmentioning

confidence: 87%

“…Their circulating insulin consists of a mixture of free and antibody-bound insulin, the concentrations of which are affected by the characteristics of their anti-insulin antibodies (Molnar et al 1972). The influence of these antibodies on daily insulin dosage (Kerp et al 1968;Hum et al 1969) and on the quality of diabetic control (Dixon et al 1975;Andersen 1975) is controversial. The relative bio-availabilities of free and bound insulin is in dispute (Dixon et al 1975;Lev-Ran 8c Ratt 1973) as is the state of the circulating free insulin (Schlichtkrull et al 1971).…”

mentioning

confidence: 97%

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The Relationship Between Circulating Free and Bound Insulin, Insulin Antibodies, Insulin Dosage and Diabetic Control in Insulin Treated Diabetics

Asplin

Hartog

Goldie

1978

Acta Endocrinologica

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Free and bound insulin concentrations, blood glucose and anti-insulin antibody binding characteristics have been determined in 100 insulin treated diabetics; median serum free insulin was 11 mU/l when fasting and rose to 30 mU/l after the mid-day meal. Significant correlation between blood glucose and serum free (but not bound) insulin was found. No relationship between insulin antibodies and daily insulin dose or diabetic control were found, nor a relationship between free insulin and antibody characteristics.Diabetics treated with conventional bovine insulin preparations develop anti¬ bodies to the injected insulin (Berson 8c Yalow 1964). Their circulating insulin consists of a mixture of free and antibody-bound insulin, the concentrations of which are affected by the characteristics of their anti-insulin antibodies (Molnar et al. 1972). The influence of these antibodies on daily insulin dosage (Kerp et al. 1968; Hum et al. 1969) and on the quality of diabetic control (Dixon et al. 1975;Andersen 1975) is controversial. The relative bio-availabilities of free and bound insulin is in dispute (Dixon et al. 1975;Lev-Ran 8c Ratt 1973) as is the state of the circulating free insulin (Schlichtkrull et al. 1971).In this paper we report measurements of serum free and bound insulin con¬ centrations and their relationships to insulin antibody binding characteristics and the prevailing blood glucose in one hundred long-term bovine insulin treated diabetics. We also attempt to relate these antibody characteristics to the quality of diabetic control and daily insulin dosage.

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Section: Discussionmentioning

confidence: 87%

mentioning

confidence: 97%

The Relationship Between Circulating Free and Bound Insulin, Insulin Antibodies, Insulin Dosage and Diabetic Control in Insulin Treated Diabetics

Asplin

Hartog

Goldie

1978

Acta Endocrinologica

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“…Tbe majority of observers found no correlation between insulin dose and antibody titer, but some found positive correlation with high affinity antibodies and negative one with antibodies of low affinity for insulin (Kerp, Kasemir and Kielding 1968). There are differences of opinion conceming the dinical significance of antibodies to various insulins in non-resistant diabetics.…”

Section: Discussionmentioning

confidence: 95%

Insulin Binding and Free Insulin in Serum of Insulin-Treated Diabetics

Lev-Ran¹,

L²

1973

Horm Metab Res

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Insulin_I l25 (0.1 ml, 0.1 microCi) was added to the equal volume of serum and fraetionated on 9 x 300 mm column of Sephadex G-I00 into protein-bound (B) and free (F) insulin immediately or after 24-hour incubation. When the fractionation was performed immediately, the B/F ratio was in 10 healthy persons 0.045 ± 0.006 (just like that of label-Ied insulin preparation itself), and in twenty-six insulintreated diabetics 0,605 ± 0,073, After incubation B/F ratio were correspondingly 0.056 ± 0.011 and 2.021 ± 0.103. There was no overlapping between normals and diabetics (P < .00l), and no protein binding of insulin by normal serum was evident. At pH 3 the bound insulin dissociated, and virtually all the radioactivity appeared with free insulin fraction. Free insulin level in the serum of diabetics as determined after serum fractionation on Sephadex G-IOO was 35.8 ± 4.0 micro V/mi only and did not conelate with B/F ratios; ,insulin dose or blood glucose. No (free)-hyperinsulinemia was found in severe insulin-treated diabetics.

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“…At present two possible effects of insulin antibodies on insulin action are discussed, namely, insulin neutralization and stabilization of carbohydrate metabolism by an insulin depot effect. Although a neutralization effect in vitro is clearly demonstrable {Ziegler, Lippmann, Fiedler and Knospe 1972), the demonstration of a relationship between daily insulin dosage and plasma insulin binding is only possible if a large number of subjects are being investigated (Kerp, Kasemir and Kieling 1968;Ortved Andersen 1972;Mustaffa, Daggett and Nabarro 1977). Dixon, Exon and Malins (1975) proposed an improved diabetic stability owing to insulin antibodies.…”

Section: Introductionmentioning

confidence: 99%

Insulin Antibodies in Juvenile Diabetes Mellitus

Keilacker¹,

Rjasanowski²,

Ziegler³

et al. 1982

Horm Metab Res

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We investigated the plasma insulin binding patterns of 15 insulin-treated juvenile diabetics with a very low residual B-cell function. Determination of the insulin binding was performed after removal of therapeutic insulin. 125I-monoiodoinsulin was used and insulin binding was measured over a large range of insulin concentration. Insulin binding parameters were evaluated by Scatchard analysis of the binding data. Significantly elevated insulin binding was detected when the diabetic control was stable. These patients had a lower insulin requirement and low equilibrium dissociation constants of the high affinity antibodies. Furthermore, a strong negative correlation between the duration of insulin treatment and insulin binding could be demonstrated resulting from both decreasing antibody affinities and maximum binding capacities. We discuss the stabilizing effect of insulin antibodies on the metabolic character assuming that dissociating insulin-antibody complexes mimic a "basal insulin secretion" similar to pancreatic B-cells with residual functional capacity.

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Insulinbindende Antikörper und Insulinbedarf bei Diabetikern

Cited by 20 publications

References 27 publications

The Relationship Between Circulating Free and Bound Insulin, Insulin Antibodies, Insulin Dosage and Diabetic Control in Insulin Treated Diabetics

The Relationship Between Circulating Free and Bound Insulin, Insulin Antibodies, Insulin Dosage and Diabetic Control in Insulin Treated Diabetics

Insulin Binding and Free Insulin in Serum of Insulin-Treated Diabetics

Insulin Antibodies in Juvenile Diabetes Mellitus

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