Intake of green-plant membrane with dietary oil suppresses postprandial hypertriglyceridemia in rats via promoting excretion of bile acids

Abstract

Green-plant membrane is a phytonutrient present in green leafy vegetables at high concentration. Postprandial increases in blood triglyceride levels result in insulin resistance and type 2 diabetes. Additionally, dietary life and eating order also affect postprandial hypertriglyceridemia. In this study, the effects of once-daily intake of green-plant membrane with dietary oil on postprandial hypertriglyceridemia were investigated in vitro and in vivo. In vitro, green-plant membrane bound hydrophobic bile acids but did not inhibit pancreatic lipase activity. Following the administration, green-plant membrane with dietary oil in rats, oral fat tolerance tests, increases in serum triglycerides levels were significantly reduced. Moreover, fecal total lipid and bile acid volumes were significantly increased in rats that administered 200 mg/mL green-plant membrane. These results suggest that green-plant membrane with dietary oil inhibits dietary fat absorption via promotion of bile acid excretion in feces and the effectiveness of eating green-plant membrane, such as green leafy vegetables, with meals.

Beneficial effects of the consumption of sun‐dried radishes ( Raphanus sativus cv. YR‐Hyuga‐Risou) on dyslipidemia in apolipoprotein E‐deficient mice

“…Oral fat tolerance tests (OFTTs) were performed according to the modified method reported by Matsuda et al (2018). After 1 week of acclimation, animals were fasted for 12 hr and randomly assigned to one of the following four treatment groups: orlistat at 60 mg/5 ml/kg body weight, RR or SDR suspension at 500 mg/5 ml/ kg body weight, or the same volume of vehicle (deionized water).…”

Effects of Thylakoid-Rich Spinach Extract on the Pharmacokinetics of Drugs in Rats

“…Clinical studies have demonstrated that thylakoid-rich spinach extract suppresses subjective hunger, promote body weight loss, and reduce low density lipoprotein cholesterol. 13,14) In rats, intake of thylakoid-rich spinach extract with dietary oil is suggested to inhibit dietary fat absorption via binding to bile acids, which promotes excretion of bile acids in feces. 15) It is also well documented that bile acids facilitate the gastrointestinal absorption of water-insoluble drugs by acting as a surfactant to enhance the drug's water solubility.…”