Integral production and concentration of surfactin from Bacillus sp. ITP-001 by semi-batch foam fractionation

Silva, Maíra Taynara Santos da; Soares, Cleide Mara Faria; Lima, Álvaro Silva; Santana, César Costapinto

doi:10.1016/j.bej.2015.04.010

Cited by 31 publications

(10 citation statements)

References 26 publications

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“…The technique consists of the insertion of gas through the liquid column, affording the gaseous phase bubbling inside the solution containing the surface-active material to be isolated [8,9]. The gas bubbles allow the emergence of a large surface area, favoring the selective adsorption of the solute in the gas-liquid interface.…”

Section: Introductionmentioning

confidence: 99%

Ascertainment of Surfactin Concentration in Bubbles and Foam Column Operated in Semi-Batch

Gonçalves

Santana

2019

Processes

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This paper describes a mathematical model for the convection, diffusion, and balance phenomena for predicting the depletion curves, i.e., variations in the timed surface-active molecule concentration for fractionation processes in bubbles and foam column, operated in semi-batch. The model was applied for the purification of the surfactin solution and the results were compared with experimental data. Gibbs adsorption curves were obtained for the biosurfactant at different temperatures, and then adjusted with estimated parameters, according to the Langmuir adsorption model. The gas bubble sizes were optically determined. The isotherm adsorption parameters and bubble average diameter are crucial for the attainment of the depletion curves, generated by the model described. The results demonstrate that the process is most effective when operating a column with reduced gas flow and low initial concentration. A top product with two or thirty times greater concentration than the initial one was achieved and the highest biosurfactant concentrations were attained for higher operating temperatures. It was also observed that bubble diameter increased with a higher gas flow. The adjustment obtained for the adsorption curves of Gibbs was satisfactory. Therefore, there was evidence that surfactin molecules adsorb in monolayers in the liquid–gas interface.

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Section: Introductionmentioning

confidence: 99%

Ascertainment of Surfactin Concentration in Bubbles and Foam Column Operated in Semi-Batch

Gonçalves

Santana

2019

Processes

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“…ITP-001 and the antimicrobial activity was determined by measuring zones of inhibition. While no antimicrobial effects were observed on S. aureus , a significant inhibition was detected against E. coli at 2300 mg

[43] . In our study, we have used a microtiter plate assay to measure the antimicrobial activity.…”

Section: Resultsmentioning

confidence: 84%

“…Santos da Silva et al [43] evaluated the antimicrobial activity of surfactin against S. aureus ATCC-6533 and E. coli CCT-0355. The biosurfactant was produced by Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies in the literature evaluate the antimicrobial activity through the agar diffusion tests, which does not allow indicating values of concentrations of antimicrobial agents and their respective inhibitory effect on microorganism growth [43] , [45] . In our study, we carried out a throughput assay using 96-well plates, allowing us to evaluate the effect of different biosurfactant concentrations on the test microorganisms.…”

Section: Resultsmentioning

confidence: 99%

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The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide

Nazareth

Zanutto

Tripathi

et al. 2020

Biotechnology Reports

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“…During the fermentation process with gas sparging aeration, surfactin was produced in the culture medium and efficiently separated and purified from culture medium by a simple foam fractionation unit. MaíraTaynara et al successfully applied the integration of a stirred and aerated tank bioreactor with a foam recovery column for the simultaneous production and concentration of surfactin. In our work, surfactin was never detected in the residual fermentation broth (or was below the limit of detection ∼10 mg L −1 ), and all of the surfactin was present in the foam.…”

Section: Discussionmentioning

confidence: 99%

Repeated batch fermentation for surfactin production with immobilizedBacillus subtilisBS-37: two-stage pH control and foam fractionation

Liu

Lin

et al. 2016

J. Chem. Technol. Biotechnol

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BACKGROUND Surfactin, a lipopeptide biosurfactant produced by Bacillus strains, is one of the most surface‐active biosurfactants, and has the potential to enhance oil recovery or environmental remediation. However, low yield and high production costs limit its use in high‐volume applications. In this work, Bacillus subtilis BS‐37 was immobilized on a piece of cotton towel in a 5 L stirred tank reactor; the fill‐and‐draw process and foam fractionation were used for repeated batch fermentations. A two‐stage pH control operation was developed for surfactin fermentation: for repeated batch fermentation, the pH value was 5.0 for the biomass accumulation in the previous 6 h; and then the pH value was raised to 7.5 for surfactin production over the following 18 h of fermentation. RESULTS The immobilized Bacillus subtilis BS‐37 cells were efficiently reused for 7 cycles, producing 7887.3 mg surfactin in total. CONCLUSIONS The repeated batch fermentation, low‐cost foam fractionation, reusable immobilized cells, and energy‐efficient open (nonsterile) fermentation could greatly promote the development of industrial production of surfactin. © 2016 Society of Chemical Industry

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Integral production and concentration of surfactin from Bacillus sp. ITP-001 by semi-batch foam fractionation

Cited by 31 publications

References 26 publications

Ascertainment of Surfactin Concentration in Bubbles and Foam Column Operated in Semi-Batch

Ascertainment of Surfactin Concentration in Bubbles and Foam Column Operated in Semi-Batch

The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide

Repeated batch fermentation for surfactin production with immobilizedBacillus subtilisBS-37: two-stage pH control and foam fractionation

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