Integrated analysis of chronic lipotoxicity on muscle metabolism and stress and its reversal by antioxidants

Verma, Mahesh Kumar; Yateesh, Aggunda Nagaraju; Smitha, Rachapalli; Neelima, Korrapati; Pallavi, Puttrevana M; Reddy, Madhusudhan; Poornima, Jayaram; Oommen, Anup Mammen; Jagannath, Madanahalli R; Somesh, Baggavalli P

doi:10.1186/2193-1801-3-251

Cited by 6 publications

(4 citation statements)

References 58 publications

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“…The mechanisms involved in this intracellular lipid accumulation and how these phenomena are involved in WB myopathy are relevant to understand WB etiology and merit further in depth investigations. It is possible that this fatty acid overload leads to oxidative stress and lipotoxicity ( Holland et al., 2007 ; Verma et al., 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Research Note: Dietary phytase reduces broiler woody breast severity via potential modulation of breast muscle fatty acid profiles

et al. 2020

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Woody breast ( WB ) myopathy is a major concern and economic burden to the poultry industry, and for which, there is no effective solution because of its unknown etiology. In a previous study, we have shown that phytase (Quantum Blue, QB ) reduces the WB severity by 5% via modulation of oxygen homeostasis-related pathways. As WB has been suggested to be associated with lipid dysmetabolism, we aimed to determine the effect of QB on WB and breast muscle fatty acid profile. Male broilers were subjected to 6 treatments (96 birds/treatment): a nutrient adequate control group (PC), the PC supplemented with 0.3% myo-inositol (PC + MI), a negative control ( NC ) deficient in available P and Ca by 0.15 and 0.16%, respectively, the NC fed with QB at 500 (NC+500 FTU), and 1,000 (NC+ 1,000 FTU) or 2,000 FTU/kg of feed (NC+2,000 FTU). Woody breast and white striping scores were recorded, and fatty acid profiles were determined using gas liquid chromatography. Woody breast-affected muscles exhibited a significant higher incidence of white striping as liquid chromatography analysis reveals an imbalance of fatty acid profile in the breast of WB-affected birds with a significant higher percent of saturated fatty acids (SFA, myristic [14:0], pentadecanoic [15:0], and margaric [17:0]) and monounsaturated fatty acids (myristoleic [14:1], palmitoleic [16:1 c ], 10-trans-heptadecenoic [17:1 t ], oleic [18:1 c 9], and vaccenic [18:1 c 11]), and lower content of polyunsaturated fatty acids (PUFA) and omega-3 ( P < 0.05). Quantum Blue at high doses (1,000 and 2,000 FTU) significantly reduces the percent of SFA and increases that of PUFA compared with the control group. In conclusion, WB myopathy seemed to be associated with an imbalance of fatty acid profile, and QB ameliorates the severity of WB potentially via modulation of SFA and PUFA contents.

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Section: Resultsmentioning

confidence: 99%

Research Note: Dietary phytase reduces broiler woody breast severity via potential modulation of breast muscle fatty acid profiles

et al. 2020

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“…Increased FFA load can cause multiple dysregulations, which are collectively known as lipotoxicity [ 23 ]. Lipotoxicity constitutes an important pathogenic link between obesity, insulin resistance, and type 2 diabetes [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Oxamate Improves Glycemic Control and Insulin Sensitivity via Inhibition of Tissue Lactate Production in db/db Mice

Zheng

Zhang

et al. 2016

PLoS ONE

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Oxamate (OXA) is a pyruvate analogue that directly inhibits the lactate dehydrogenase (LDH)-catalyzed conversion process of pyruvate into lactate. Earlier and recent studies have shown elevated blood lactate levels among insulin-resistant and type 2 diabetes subjects and that blood lactate levels independently predicted the development of incident diabetes. To explore the potential of OXA in the treatment of diabetes, db/db mice were treated with OXA in vivo. Treatment of OXA (350–750 mg/kg of body weight) for 12 weeks was shown to decrease body weight gain and blood glucose and HbA1c levels and improve insulin secretion, the morphology of pancreatic islets, and insulin sensitivity in db/db mice. Meanwhile, OXA reduced the lactate production of adipose tissue and skeletal muscle and serum lactate levels and decreased serum levels of TG, FFA, CRP, IL-6, and TNF-α in db/db mice. The PCR array showed that OXA downregulated the expression of Tnf, Il6, leptin, Cxcr3, Map2k1, and Ikbkb, and upregulated the expression of Irs2, Nfkbia, and Pde3b in the skeletal muscle of db/db mice. Interestingly, LDH-A expression increased in the islet cells of db/db mice, and both treatment of OXA and pioglitazone decreased LDH-A expression, which might be related to the improvement of insulin secretion. Taken together, increased lactate production of adipose tissue and skeletal muscle may be at least partially responsible for insulin resistance and diabetes in db/db mice. OXA improved glycemic control and insulin sensitivity in db/db mice primarily via inhibition of tissue lactate production. Oxamic acid derivatives may be a potential drug for the treatment of type 2 diabetes.

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“…Cell lysates were centrifuged at 1,000 rpm for 10 min and the clear supernatants were incubated with acetoacetyl CoA for 1 hr. The reduction of NADH levels was monitored by measuring the absorbance at 340 nm, and enzyme activity was calculated based on this (Lynen, ; Verma et al, ). The enzyme activity was normalized to corresponding protein content in the lysate.…”

Section: Methodsmentioning

confidence: 99%

The nutritional supplement FFD-002 attenuates streptozotocin-induced diabetes and decelerates diabetes-related tissue injury

Kumar¹,

Azeemuddin²,

Rao³

et al. 2017

J Food Biochem

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A nutritional supplement FFD-002 composed of micronutrients, macronutrients, and herbal actives was formulated with ingredients known for therapeutic effect in diabetes management. The study evaluated the effect of supplementing FFD-002 on diabetes and related complications. Diabetes was induced in Wistar rats by administering a single dose of streptozotocin (STZ, 45 mg/Kg bodyweight, intraperitoneal) and rats were supplemented with either 2.5 g/kg or 5 g/kg of FFD-002 (p. o [per oral] once daily) for 90 days. Supplementation of FFD-002 to diabetic rats resulted in improved fasting glucose levels and marked decrease in diabetes-induced nephropathy and retinopathy. Cell-based experimental results indicated that FFD-002 treatment increased fat oxidation in muscle cells and stimulated the secretion of GLP-1 (Glucagon-Like Peptide-1) in intestinal cells.These studies showed that FFD-002, formulated by combining modern nutritional concepts with phytomedicine, could be beneficial in the management of diabetes.

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Integrated analysis of chronic lipotoxicity on muscle metabolism and stress and its reversal by antioxidants

Cited by 6 publications

References 58 publications

Research Note: Dietary phytase reduces broiler woody breast severity via potential modulation of breast muscle fatty acid profiles

Research Note: Dietary phytase reduces broiler woody breast severity via potential modulation of breast muscle fatty acid profiles

Oxamate Improves Glycemic Control and Insulin Sensitivity via Inhibition of Tissue Lactate Production in db/db Mice

The nutritional supplement FFD-002 attenuates streptozotocin-induced diabetes and decelerates diabetes-related tissue injury

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