Integrated analysis of miRNA-mRNA regulatory network and functional verification of miR-338-3p in coronary heart disease

Qi, Jie; Han, Wenqi; Zhong, Nier; Gou, Qiling; Sun, Chaofeng

doi:10.1007/s10142-022-00941-w

Cited by 9 publications

(1 citation statement)

References 35 publications

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“…Coronary heart disease (CHD) also called ischemic heart disease (IHD), one of the most serious cardiovascular disease 1 , contributing significantly to global mortality and morbidity rates 2 . Stable ischemic heart disease (SIHD) is a syndrome marked by stable angina, which refers to pain or pressure brought on reliably by exertion or emotion and relieved by rest or nitroglycerin 3 .…”

Section: Introductionmentioning

confidence: 99%

Short-term exposure to various ambient air pollutants and emergency department visits for cause-stable ischemic heart disease: a time-series study in Shanghai, China

Zhou,

Jin,

Zhang

2023

Sci Rep

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Studying the impact of local meteorological conditions and air pollution on cardiovascular disease is crucial for reducing the burden of cardiovascular disease. However, there have been few studies on the acute effects of various air pollutants on stable ischemic heart disease (SIHD), and the effects of these factors are not well defined and require further investigation. We performed a time-series study aimed at exploring the association between short-term exposure to various air pollutants and emergency department (ED) visits for SIHD during 2013–2020 in Baoshan District Renhe Hospital of Shanghai, China. The associations between air pollution (NO2, PM2.5, PM10, SO2 O3-8 h and CO) and ED visits were analyzed using quasi-Poisson regression. Subgroup and sensitivity analyses were conducted. From 2013 to 2020, a total of 18,241 ED visits for SIHD were recorded. Elevated PM2.5, PM10, NO2, SO2 and CO were significantly associated with increased ED visits for SIHD at lag (0, 5), lag 0, lag (0-4, 01-03), lag (0-3, 5, 01-03) and lag (3-5). When the concentration of O3-8 h was lower than the threshold recommended by the WHO, exposure to O3-8 h was associated with a slightly decreased risk of SIHD. Moreover, the relationship between different types of air pollution and the frequency of ED visits exhibited variations based on gender, age, and seasonality. This study suggests that short-term exposure to PM2.5, PM10, NO2, SO2 and CO might induce SIHD, especially in old females. Air pollution control measures should be encouraged to prevent the occurrence and development of SIHD.

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Section: Introductionmentioning

confidence: 99%

Short-term exposure to various ambient air pollutants and emergency department visits for cause-stable ischemic heart disease: a time-series study in Shanghai, China

Zhou,

Jin,

Zhang

2023

Sci Rep

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RNA-seq and microRNA association analysis to explore the pathogenic mechanism of DHAV-1 infection with DEHs

Wang

Zhang

et al. 2023

Funct Integr Genomics

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Analysis of the regulatory role of miR-34a-5p/PLCD3 in the progression of osteoarthritis

Ying

Jiang

et al. 2023

Funct Integr Genomics

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Osteoarthritis is a heterogeneous disease with complex etiology. However, there is no effective treatment strategy at present. The purpose of this study is to explore the miRNA-mRNA regulatory network and molecular mechanism that regulate the progression of osteoarthritis. In this article, we downloaded dataset (GSE55457, GSE82107, GSE143514 and GSE55235) from Gene Expression Omnibus (GEO) to screen differentially expressed mRNAs in osteoarthritis. Then, through weighted gene co-expression network analysis (WGCNA), functional enrichment, protein-protein interaction networks (PPI), miRNA-mRNA co-expression network, ROC curve, immune in ltration analysis and qPCR, the mRNA PLCD3, which was highly expressed in osteoarthritis and had clinical predictive value, was screened. Whereafter, we found that PLCD3 directly targets miR-34a-5p through DIANA and dual luciferase experiment. And the expression of PLCD3 and miR-34a-5p were negatively correlated. In addition, CCK-8 and wound healing showed that miR-34a-5p mimic inhibited hFLS-OA cell proliferation and promoted hFLS-OA cell migration.PLCD3 overexpression showed an opposite trend. Western further found that overexpression of miR-34a-5p reduced the protein expression levels of p-PI3K and p-AKT, while overexpression of PLCD3 also showed the opposite trend. Besides, combined with the effect of PI3K/AKT pathway inhibitor BIO(IC50 = 5.95 µM), the results showed that overexpression of miR-34a-5p increased the inhibitory effect of BIO on p-PI3K and p-AKT protein expression, while overexpression of PLCD3 signi cantly reversed the inhibitory effect. In all, the miR-34a-5p/PLCD3 axis may mediate the PI3K/AKT pathway in regulating cartilage homeostasis in synovial osteoarthritis. These data indicate that miR-34a-5p/PLCD3 may be a new prognostic factor in the pathology of synovial osteoarthritis.

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Integrated analysis of miRNA-mRNA regulatory network and functional verification of miR-338-3p in coronary heart disease

Cited by 9 publications

References 35 publications

Short-term exposure to various ambient air pollutants and emergency department visits for cause-stable ischemic heart disease: a time-series study in Shanghai, China

Short-term exposure to various ambient air pollutants and emergency department visits for cause-stable ischemic heart disease: a time-series study in Shanghai, China

RNA-seq and microRNA association analysis to explore the pathogenic mechanism of DHAV-1 infection with DEHs

Analysis of the regulatory role of miR-34a-5p/PLCD3 in the progression of osteoarthritis

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