Integrated analysis of transcriptomic and proteomic data from tree peony (P. ostii) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism

Wang, Xiaojing; Liang, Haiying; Guo, Da‐Long; Guo, Lili; Duan, Xiangguang; Jia, Qishi; Hou, Xiaogai

doi:10.1038/s41438-019-0194-7

Cited by 86 publications

(68 citation statements)

References 67 publications

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“…4 and 5). In a previous study, genes related to fatty acid biosynthesis were grouped into two categories and detected in the early or later stages of seed development in tree peony [49]. Similarly, the coordinated regulation of multiple genes has been shown to promote the high accumulation of longchain unsaturated fatty acids in the seeds of tea oil camellia [50], and consistent results have been obtained in Brassica napus [51,52], Glycine max [53], and Linum usitatissimum [54].…”

Section: Discussionmentioning

confidence: 61%

De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism

et al. 2020

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De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism Abstract Background: Zanthoxylum armatum (Z. armatum) is a highly economically important tree that presents a special numbing taste. However, the underlying regulatory mechanism of the numbing taste remains poorly understood. Thus, the elucidation of the key genes associated with numbing taste biosynthesis pathways is critical for providing genetic information on Z. armatumand the breeding of high-quality germplasms of this species.Results: Here, de novo transcriptome assembly was performed for the five major organs of Z. armatum, including the roots, stems, leaf buds, mature leaves and fruits. A total of 111,318 unigenes were generated with an average length of 1014 bp. Additionally, a large number of SSRs were obtained to improve our understanding of the phylogeny and genetics of Z. armatum. The organ-specific unigenes of the five major samples were screened and annotated via GO and KEGG enrichment analysis. A total of 53 and 34 unigenes that were exclusively upregulated in fruit samples were identified as candidate unigenes for terpenoid biosynthesis or fatty acid biosynthesis, elongation and degradation pathways, respectively. Moreover, 40 days after fertilization (Fr4 stage) could be an important period for the accumulation of terpenoid compounds during the fruit development and maturation of Z. armatum. The Fr4 stage could be a key point at which the first few steps of the fatty acid biosynthesis process are promoted, and the catalysis of subsequent reactions could be significantly induced at 62 days after fertilization (Fr6 stage). Conclusions: The present study realized de novo transcriptome assembly for the five major organs of Z. armatum. To the best of our knowledge, this study provides the first comprehensive analysis revealing the genes underlying the special numbing taste of Z. armatum. The assembled transcriptome profiles expand the available genetic information on this species and will contribute to gene functional studies, which will aid in the engineering of high-quality cultivars of Z. armatum.

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Section: Discussionmentioning

confidence: 61%

De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism

et al. 2020

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“…At present, the research has focused on the analysis of fatty acid in tree peony seed oils [2][3][4]. Another category of important nonpolar compounds, phytosterols, has not been reported in tree peony seed oil to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

Integrated Profiling of Fatty Acids, Sterols and Phenolic Compounds in Tree and Herbaceous Peony Seed Oils: Marker Screening for New Resources of Vegetable Oil

Wang

Contreras

et al. 2020

Foods

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Tree peonies (Paeonia ostii and Paeonia rockii) are popular ornamental plants. Moreover, these plants have become oil crops in recent years. However, there are limited compositional studies focused on fatty acids. Therefore, this work aims to reveal compositional characteristics, regarding fatty acids, sterols, γ-tocopherol and phenolic compounds, of tree peony seed oils from all major cultivation areas in China, and to compare with herbaceous peony seed oil. For that, an integrative analysis was performed by GC-FID, GC-MS and UHPLC-Q-TOF-MS technologies. The main fatty acid was α-linolenic acid (39.0–48.3%), while β-sitosterol (1802.5–2793.7 mg/kg) and fucosterol (682.2–1225.1 mg/kg) were the dominant phytosterols. Importantly, 34 phenolic compounds, including paeonol and “Paeonia glycosides” (36.62–103.17 μg/g), were characterized in vegetable oils for the first time. Conclusively, this work gives new insights into the phytochemical composition of peony seed oil and reveals the presence of bioactive compounds, including “Paeonia glycosides”.

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“…There are nine wild species in section Moutan DC, which differ with respect to preferred habitat, but all rich in unsaturated FAs (>90%) and ALA (26.7-50%) [6,9]. To date, transcriptomic, proteomic, and microRNA sequencing have been used to investigate the mechanisms underlying ALA synthesis in tree peony seeds [10][11][12][13][14]. Previous studies have focused on enzymes that are key to ALA biosynthesis in various tree peony species, including FAD3 in P. rockii, P. potaninii and P. lutea seed [15]; FAD2 and FAD8 in P. ostii [10]; and ACCase, FATA, LPCAT, FADs, and DGAT in the developing endosperm of P. ostii var.…”

Section: Introductionmentioning

confidence: 99%

“…lishizhenii [11]. Wang et al [14] identified 115 genes and 24 proteins associated with the ALA metabolism in P. ostii seeds. However, despite these studies of FA metabolism in the tree peony, additional functional genes require further exploration.…”

Section: Introductionmentioning

confidence: 99%

“…Zhang et al [13,15] compared FAD transcription (i.e., SAD, FAD2, and FAD3) among three species of tree peonies, and found that FAD transcription was greater in P. rockii seeds than in P. potaninii and P. lutea seeds. In addition, various transcriptomes of P. ostii seeds or endosperms are also available [10,11,14]. However, to date, no integrated analysis of the developing pericarp, seed coat, and endosperm in P. ostii has been performed.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of a-linolenic acid content and biosynthesis in Paeonia ostii fruits and seeds

Zhang

et al. 2020

Preprint

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Background Paeonia ostii is a potentially important oilseed crop because its seed yield is high and the seeds are rich in α-linolenic acid (ALA). However, the molecular mechanisms underlying ALA biosynthesis during seed kernel, seed testa, and fruit pericarp development in this plant are unclear. We used transcriptome data to address this knowledge gap. Results Gas chromatograph-mass spectrometry indicated that ALA content was highest in the kernel, moderate in the testa, and lowest in the pericarp. Therefore, we used RNA-sequencing to compare ALA synthesis among these three tissues. We identified 227,837 unigenes, with an average length of 755 bp. Of these, 1371 unigenes were associated with lipid metabolism. The fatty acid (FA) biosynthesis and metabolism pathways were significantly enriched during the early stages of oil accumulation in the kernel. ALA biosynthesis was significantly enriched in parallel with increasing ALA content in the testa, but these metabolic pathways were not significantly enriched during pericarp development. By comparing unigene transcription profiles with patterns of ALA accumulation, specific unigenes encoding crucial enzymes and transcription factors (TFs) involved in de novo FA biosynthesis and oil accumulation were identified. Specifically, the bell-shaped expression patterns of genes encoding SAD, FAD2, FAD3, PDCT, PDAT, OLE, CLE, and SLE in the kernel were similar to the patterns of ALA accumulation in this tissue. Genes encoding BCCP, BC, KAS I– III, and FATA were also upregulated during the early stages of oil accumulation in the kernel. In the testa, the upregulation of the genes encoding SAD, FAD2, and FAD3 was followed by a sharp increase in the concentrations of ALA. In contrast, these genes were minimally expressed (and ALA content was low) throughout pericarp development. Conclusions In this study, we used three tissues with high, moderate, and low ALA concentrations as an exemplar system in which to compare tissue-specific ALA accumulation mechanism in P. ostii. The genes and TFs identified herein might be useful targets for future studies of ALA accumulation in the tree peony. This study also provides a framework for future studies of FA biosynthesis in other oilseed plants.

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Integrated analysis of transcriptomic and proteomic data from tree peony (P. ostii) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism

Cited by 86 publications

References 67 publications

De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism

De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism

Integrated Profiling of Fatty Acids, Sterols and Phenolic Compounds in Tree and Herbaceous Peony Seed Oils: Marker Screening for New Resources of Vegetable Oil

Transcriptomic analysis of a-linolenic acid content and biosynthesis in Paeonia ostii fruits and seeds

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