Integrated assessment of differentially expressed plasma microRNAs in subtypes of nonsyndromic orofacial clefts

Abstract

Supplemental Digital Content is available in the text

miRNAs as biomarkers of orofacial clefts: A systematic review

“…Specific biological functions attributed to these miRNAs include palate and/ or anatomical development 15,17 and regulation of Wnt, Notch, and Hedgehog signaling pathways. 16 Considering epigenetics is still a recent approach in palatogenesis research, our results provide a better understanding of the roles of miRNAs in this scenario and aim to identify the potential clinical applications that this group of molecules may allow.…”

“…MiRNAs participate in growth and development through gene expression regulation and may have an impact on the process of palatogenesis in mammals by controlling gene networks . Several miRNAs have already been identified as being involved in congenital diseases.…”

“…MiRNAs participate in growth and development through gene expression regulation and may have an impact on the process of palatogenesis in mammals by controlling gene networks. 15,16,18 Several miRNAs have already been identified as being involved in congenital diseases. Recent evidence from research on zebrafish 19 and mice 20 indicate that these molecules are critical factors in both normal palatogenesis and cleft palate formation.…”

“…Three articles were classified as having unclear methodology since they did not explicit time between blood collection and the first centrifugation step. [15][16][17] This is one of the pre-analytical variables that may compromise reproducibility of the results, as it affects miRNA stability and allows high false-positive or negative rates due to the hemolysis effect. Additionally, one article did not indicate participant age range, an aspect that could bias results.…”

Extracellular Matrix Composition and Remodeling: Current Perspectives on Secondary Palate Formation, Cleft Lip/Palate, and Palatal Reconstruction

“…Many different microRNAs have been identified to temporally and spatially regulate morphogens and transcription factors during palatogenesis (Eberhart et al, 2008;Seelan et al, 2014;Ding et al, 2016;Reiss and Bhakdi, 2017;Schoen et al, 2017). Not surprisingly, microRNAs have been suggested as be new targets for investigating in CL/P studies (Li et al, 2010;Wang et al, 2013Wang et al, , 2017Ma et al, 2014;Gao et al, 2015;Li D. et al, 2016;Li J. et al, 2016;Schoen et al, 2017Schoen et al, , 2018Chen et al, 2018;Grassia et al, 2018;Pan et al, 2018;Suzuki et al, 2018;Wu N. et al, 2018;Xu M. et al, 2018).…”