Integrated assessment of toxic effects of maghemite (γ-Fe2O3) nanoparticles in zebrafish

Villacis, Rolando A R; Filho, José Sousa; Piña, Benjamı́n; Azevedo, Ricardo Bentes; Pic‐Taylor, Aline; Mazzeu, Juliana Forte; Grisólia, César Koppe

doi:10.1016/j.aquatox.2017.08.004

Cited by 61 publications

(18 citation statements)

References 52 publications

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“…According to several studies, zebrafish and their embryos have great potential as models to evaluate nanotoxicity, serving as alternatives in the approach for testing nanomaterials [73,76]. Moreover, the zebrafish model provides various means to measure nanotoxicity, such as the quick assessment of productive toxicity, teratogenicity, and developmental toxicity, as well as for evaluating immunotoxicity, genotoxicity, and neurotoxicity [7,[89][90][91][92]. For example, zebrafish were employed to evaluate nanoparticle-induced adverse effects.…”

Section: Zebrafish Modelmentioning

confidence: 99%

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

Chen

Liao

et al. 2020

IJMS

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Nanotechnology has rapidly promoted the development of a new generation of industrial and commercial products; however, it has also raised some concerns about human health and safety. To evaluate the toxicity of the great diversity of nanomaterials (NMs) in the traditional manner, a tremendous number of safety assessments and a very large number of animals would be required. For this reason, it is necessary to consider the use of alternative testing strategies or methods that reduce, refine, or replace (3Rs) the use of animals for assessing the toxicity of NMs. Autophagy is considered an early indicator of NM interactions with cells and has been recently recognized as an important form of cell death in nanoparticle-induced toxicity. Impairment of autophagy is related to the accelerated pathogenesis of diseases. By using mechanism-based high-throughput screening in vitro, we can predict the NMs that may lead to the generation of disease outcomes in vivo. Thus, a tiered testing strategy is suggested that includes a set of standardized assays in relevant human cell lines followed by critical validation studies carried out in animals or whole organism models such as C. elegans (Caenorhabditis elegans), zebrafish (Danio rerio), and Drosophila (Drosophila melanogaster)for improved screening of NM safety. A thorough understanding of the mechanisms by which NMs perturb biological systems, including autophagy induction, is critical for a more comprehensive elucidation of nanotoxicity. A more profound understanding of toxicity mechanisms will also facilitate the development of prevention and intervention policies against adverse outcomes induced by NMs. The development of a tiered testing strategy for NM hazard assessment not only promotes a more widespread adoption of non-rodent or 3R principles but also makes nanotoxicology testing more ethical, relevant, and cost- and time-efficient.

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Section: Zebrafish Modelmentioning

confidence: 99%

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

Chen

Liao

et al. 2020

IJMS

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“…DNA is susceptible to damage following exposure to many chemical entities, which results in gene mutations and larger chromosomal alterations, collectively termed genotoxicity [ 34 ]. This can be assessed in embryos, larvae or adult tissues in a number of approaches, including quantitative RAPD-PCR methodology that has been used to demonstrate dose-dependent genotoxicity of TiO 2 NPs [ 35 ], and comet assays for examining the impact of ferric oxide (Fe 2 O 3 ) NPs [ 36 ].…”

Section: Approaches For Measuring Nanoparticle Toxicity In Zebrafimentioning

confidence: 99%

Zebrafish as a Model to Evaluate Nanoparticle Toxicity

2018

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Nanoparticles are increasingly being developed for in vivo use, from targeted drug delivery to diagnostics, where they have enormous potential, while they are also being used for a variety of applications that can result in environmental exposure for humans. Understanding how specific nanoparticles interact with cells and cell systems is essential to gauge their safety with respect to either clinical or environmental exposure. Zebrafish is being increasingly employed as a model to evaluate nanoparticle biocompatibility. This review describes this model and how it can be used to assess nanoparticle toxicity at multiple levels, including mortality, teratogenicity, immunotoxicity, genotoxicity, as well as alterations in reproduction, behavior and a range of other physiological readouts. This review also provides an overview of studies using this model to assess the toxicity of metal, metal oxide and carbon-based nanoparticles. It is anticipated that this information will inform research aimed at developing biocompatible nanoparticles for a range of uses.

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“…In addition, the increasing release of NPs in the environment in the future is predicted [4]. NPs may enter both aquatic and ground/soil environments through the direct use due to planned release for toxicity elimination, wastewater treatment, spillages, and deposition from the air [5,6]. For instance, magnetite displays unique electric and magnetic properties through both Fe…”

Section: Introductionmentioning

confidence: 99%

Plant Explants Grown on Medium Supplemented with Fe₃O₄ Nanoparticles Have a Significant Increase in Embryogenesis

Kokina

Mickeviča

Jahundoviča

et al. 2017

Journal of Nanomaterials

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Development of nanotechnology leads to the increasing release of nanoparticles in the environment that results in accumulation of different NPs in living organisms including plants. This can lead to serious changes in plant cultures which leads to genotoxicity. The aims of the present study were to detect if iron oxide NPs pass through the flax cell wall, to compare callus morphology, and to estimate the genotoxicity in Linum usitatissimum L. callus cultures induced by different concentrations of Fe 3 O 4 nanoparticles. Two parallel experiments were performed: experiment A, where flax explants were grown on medium supplemented with 0.5 mg/l, 1 mg/l, and 1.5 mg/l Fe 3 O 4 NPs for callus culture obtaining, and experiment B, where calluses obtained from basal MS medium were transported into medium supplemented with concentrations of NPs identical to experiment A. Obtained results demonstrate similarly in both experiments that 25 nm Fe 3 O 4 NPs pass into callus cells and induce low toxicity level in the callus cultures. Nevertheless, calluses from experiment A showed 100% embryogenesis in comparison with experiment B where 100% rhizogenesis was noticed. It could be associated with different stress levels and adaptation time for explants and calluses that were transported into medium with Fe 3 O 4 NPs supplementation.

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Integrated assessment of toxic effects of maghemite (γ-Fe2O3) nanoparticles in zebrafish

Cited by 61 publications

References 52 publications

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

Zebrafish as a Model to Evaluate Nanoparticle Toxicity

Plant Explants Grown on Medium Supplemented with Fe₃O₄ Nanoparticles Have a Significant Increase in Embryogenesis

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Integrated assessment of toxic effects of maghemite (γ-Fe2O3) nanoparticles in zebrafish

Cited by 61 publications

References 52 publications

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

Zebrafish as a Model to Evaluate Nanoparticle Toxicity

Plant Explants Grown on Medium Supplemented with Fe3O4 Nanoparticles Have a Significant Increase in Embryogenesis

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Product

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Plant Explants Grown on Medium Supplemented with Fe₃O₄ Nanoparticles Have a Significant Increase in Embryogenesis