Integrated Biomonitoring of Dioxin-like Compounds for Waste Management and Environment.

Fast-track DRESSA: a bioassay for fast, sensitive, and selective detection of halogenated and polycyclic aromatic hydrocarbons

“…1B). This contrasts with other bioassays that usually require more than 6 £ 10 4 cells per sample [8,10,11]. Using lower concentrations of TCDD (0.1 and 0.5 pM), we also conWrmed that the detection sensitivity of the fasttrack DRESSA was not improved even if cell numbers higher than 5000 per well (10,000 or 20,000 cells/well) were used (data not shown).…”

“…Within the range between 0.5 and 100 pM, both fold induction and absolute value of SEAP activity increased in a dose-dependent manner. In previously reported bioassays, the detection limits for TCDD are pM orders [8,10]. The fast-track DRESSA achieves both higher detection sensitivity and shorter assay time.…”

“…Furthermore, we also suggested a possibility that the whole assay time may be signiWcantly reduced by modiWcation of the assay process. In currently available bioassays and the prototype DRESSA, more than 2 days are required for the whole assay including preincubation of cells, exposure to samples, and enzyme reaction [7,8]. However, we found that, in the DRESSA assay, preincubation of cells may be unnecessary and that test samples could be added immediately after cell seeding [7].…”

Identifying Toxicologically Significant Compounds in Urban Wildfire Ash Using In Vitro Bioassays and High-Resolution Mass Spectrometry

“…Dioxin-like activity of chemicals is mediated by the aryl hydrocarbon (dioxin) receptor (AhR), a nuclear receptor which binds dioxins and related chemicals with high affinity and stimulates the expressions of a battery of genes, including cytochrome P4501A1 (CYP1A1) . Recombinant cell lines containing an AhR-responsive firefly luciferase reporter gene have been extensively utilized as a simple and sensitive bioassay for the detection and relative quantitation of dioxin-like chemicals, PAHs, and related AhR activators in diverse environmental extracts, including ash. − Similarly, the ability of environmental chemicals to modulate the activity of estrogen and androgen receptors (ER and AR, respectively), nuclear receptors that play a key role in regulating the expression of genes important in reproductive and other biological effects, have been linked to a variety of adverse endocrine disruption effects. ,, Recombinant cell lines containing ER- or AR-responsive luciferase reporter genes have been developed and utilized for the detection of activators and inhibitors of these receptor signaling pathways in the extracts of environmental matrices, including atmospheric particulate materials, and environmental contaminants such as PAHs. ,− AhR-, ER-, and AR-responsive cell lines were used to assess the ability of wildfire extracts to produce estrogenic, androgenic, and dioxin-like activities. A bioassay consisting of human U937-derived macrophages was used to test the potential activity of wildfire samples to induce inflammatory biomarkers which are relevant factors in mediating human health effects including lung injuries and cardiovascular diseases.…”

Nachweis von Dioxinen und dioxinähnlichen Substanzen mit dem Micro-EROD-Bioassay: Untersuchungen von Milch und Rinderfett

“…Die Halbwertzeit der Elimination aus dem Körper beträgt 3 bis 27 Jahre (GESTIS, 2005). Es wurden endokrine Effekte, Immuntoxizität und Karzinogenität beobachtet (Sakai und Takigami, 2003). In der Regel ist die Entgiftung von Fremdstoffen durch körpereigene Enzymsysteme erfolgreich.…”